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An efficient method for medium throughput screening of cuticular wax composition in different plant species

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An efficient method for medium throughput screening of cuticular wax composition in different plant species

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dc.contributor.author Fernández Moreno, Josefina Patricia es_ES
dc.contributor.author Malitsky, S es_ES
dc.contributor.author Lashbrooke, J es_ES
dc.contributor.author Biswal, Ajaya Kumar es_ES
dc.contributor.author Racovita, Radu C. es_ES
dc.contributor.author Mellerowicz, Ewa J. es_ES
dc.contributor.author Jetter, Reinhard es_ES
dc.contributor.author Orzáez Calatayud, Diego Vicente es_ES
dc.contributor.author Aharoni, A es_ES
dc.contributor.author Granell Richart, Antonio es_ES
dc.date.accessioned 2017-09-18T08:50:11Z
dc.date.available 2017-09-18T08:50:11Z
dc.date.issued 2016-04
dc.identifier.issn 1573-3882
dc.identifier.uri http://hdl.handle.net/10251/87408
dc.description.abstract [EN] Introduction Most aerial plant organs are covered by a cuticle, which largely consists of cutin and wax. Cuticular waxes are mixtures of dozens of compounds, mostly very-long-chain aliphatics that are easily extracted by solvents. Over the last four decades, diverse cuticular wax analysis protocols have been developed, most of which are complex and time-consuming, and need to be adapted for each plant species or organ. Plant genomics and breeding programs often require mid-throughput metabolic phenotyping approaches to screen large numbers of individuals and obtain relevant biological information. Objectives To generate a fast, simple and user-friendly methodology able to capture most wax complexity independently of the plant, cultivar and organ. Methods Here we present a simple GC-MS method for screening relatively small wax amounts, sampled by short extraction with a versatile, uniform solvent. The method will be tested and validated in leaves and fruits from three different crop species: tomato (Solanum lycopersicum), apple (Malus domestica) and hybrid aspen (Populus tremula x tremuloides). Results Consistent results were obtained in tomato cultivar M82 across three consecutive years (2010-2012), two organs (leaf and fruit), and also in two different tomato (M82 and MicroTom) and apple (Golden Delicious and Granny Smith) cultivars. Our results on tomato wax composition match those reported previously, while our apple and hybrid aspen analyses provide the first comprehensive cuticular wax profile of these species. Conclusion This protocol allows standardized identification and quantification of most cuticular wax components in a range of species. es_ES
dc.description.sponsorship Research at the IBMCP was supported by MINECO Grant BIO2013-42193-R and from EC H2020 TRADITOM SFS7a-2014- (contract 634561) to Antonio Granell and by FPU-MECD personal Grant to Josefina Patricia Fernandez Moreno (AP-2007-01905). Research at the Weizmann Institute of Sciences was supported by the Israel Science Foundation (ISF) personal Grant to Asaph Aharoni (ISF Grant No. 646/11). We also thank COST FA1106 Quality Fruit for funding networking activities.
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation.ispartof Metabolomics es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Metabolic profiling es_ES
dc.subject Cuticular waxes es_ES
dc.subject Fruit surface es_ES
dc.subject Fleshy fruit es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title An efficient method for medium throughput screening of cuticular wax composition in different plant species es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s11306-016-0982-0
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BIO2013-42193-R/ES/GREEN SWITCHES: DISEÑO DE CIRCUITOS GENETICOS ARTIFICIALES PARA LA PRODUCCION DE PROTEINAS RECOMBINANTES Y EL ENRIQUECIMIENTO NUTRICIONAL DE PLANTAS SOLANACEAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/634561/EU/Traditional tomato varieties and cultural practices: a case for agricultural diversification with impact on food security and health of European population/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ISF//646%2F11/
dc.relation.projectID info:eu-repo/grantAgreement/MEC//AP2007-01905/ES/AP2007-01905/
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.description.bibliographicCitation Fernández Moreno, JP.; Malitsky, S.; Lashbrooke, J.; Biswal, AK.; Racovita, RC.; Mellerowicz, EJ.; Jetter, R.... (2016). An efficient method for medium throughput screening of cuticular wax composition in different plant species. Metabolomics. 12(4). https://doi.org/10.1007/s11306-016-0982-0 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1007/s11306-016-0982-0 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 12 es_ES
dc.description.issue 4 es_ES
dc.relation.senia 331822 es_ES
dc.identifier.eissn 1573-3890
dc.contributor.funder European Commission
dc.contributor.funder Ministerio de Economía y Competitividad
dc.contributor.funder Israel Science Foundation
dc.contributor.funder Ministerio de Educación y Ciencia es_ES
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