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dc.contributor.author | Rabissi, Agnese | es_ES |
dc.contributor.author | Vilela, Belmiro | es_ES |
dc.contributor.author | Lumbreras, Victoria | es_ES |
dc.contributor.author | Ludevid, Dolors | es_ES |
dc.contributor.author | Culiañez Macia, Francisco Antonio | es_ES |
dc.contributor.author | Pagés, Montserrat | es_ES |
dc.date.accessioned | 2017-09-20T09:47:24Z | |
dc.date.available | 2017-09-20T09:47:24Z | |
dc.date.issued | 2016-12 | |
dc.identifier.issn | 0168-9452 | |
dc.identifier.uri | http://hdl.handle.net/10251/87610 | |
dc.description.abstract | [EN] In order to identify potential substrates of the maize kinase in the ABA signalling network, ZmOST1 was used as bait against a library of cDNAs from dehydrated young leaves. A ZmOST1-interactive polypeptide ZmKS (gene locus tag: GRMZM2G114873), showing homology with the Arabidopsis thaliana basic helix-loop-helix (bHLH) DNA-binding transcription factor was identified. Using a comparative genomic approach, the ZmKS corresponding protein was identified as conceptual translated bHLH transcription factor ABA-responsive kinase substrate. ZmKS is localized in the nucleus, shows a potential binding specificity preferentially detectable on cis-acting E-box like heptameric motifs CCACTTG and CAAGTTG, and is phosphorylated by maize protein kinase ZmOST1. ZmKS is expressed in embryo, leaf and root, expression being affected by ABA and osmotic stress. Transgenic Arabidopsis plants, with gain of ZmKS function, show a delay in germination and a transcriptional stomata] opening-facilitator activity, switchover upon ZmKS phosphorylation, suggesting that ZmKS is an ABA-repressed trans-acting activator. (C) 2016 Elsevier Ireland Ltd. All rights reserved. | es_ES |
dc.description.sponsorship | We greatly appreciate the comments and language help provided by Drs at Curtin University (WA, Australia): Jose A. Caparros-Martin, Jorge Martinez and Corey Giles. Research in M.P laboratory was supported grant from the Spanish Ministry of Science and Education BIO2012-3186 Centre de Recerca en Agri-genomica 20 (CRAG), Consortium CSIC-IRTA-UAB-UB. Campus UAB - Edifici CRAG, 21 Cerdanyola del Valles 08193 Barcelona (Spain), Tel: +34 935636600, Fax: 22 +34935636601 | en_EN |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.ispartof | Plant Science | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Zea mays | es_ES |
dc.subject | bHLH transcription factor | es_ES |
dc.subject | ABA-repressed | es_ES |
dc.subject | Stomatal activity | es_ES |
dc.subject | Abiotic stress | es_ES |
dc.title | Molecular characterization of maize bHLH transcription factor (ZmKS), a new ZmOST1 kinase substrate | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.plantsci.2016.09.003 | |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//BIO2012-31860/ES/GENES REGULADOS POR ABA Y ESTRES ABIOTICO EN PLANTAS/ | es_ES |
dc.rights.accessRights | Cerrado | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Rabissi, A.; Vilela, B.; Lumbreras, V.; Ludevid, D.; Culiañez Macia, FA.; Pagés, M. (2016). Molecular characterization of maize bHLH transcription factor (ZmKS), a new ZmOST1 kinase substrate. Plant Science. 253:1-12. https://doi.org/10.1016/j.plantsci.2016.09.003 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://doi.org/10.1016/j.plantsci.2016.09.003 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 12 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 253 | es_ES |
dc.relation.senia | 331839 | es_ES |
dc.identifier.pmid | 27968978 | |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |