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Small RNAs containing the pathogenic determinant of a chloroplast-replicating viroid guide the degradation of a host mRNA as predicted by RNA silencing

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Small RNAs containing the pathogenic determinant of a chloroplast-replicating viroid guide the degradation of a host mRNA as predicted by RNA silencing

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dc.contributor.author Navarro, B es_ES
dc.contributor.author Gisel, A es_ES
dc.contributor.author Rodio, Maria Elena es_ES
dc.contributor.author Delgado Villar, Sonia Guadalupe es_ES
dc.contributor.author Flores Pedauye, Ricardo es_ES
dc.contributor.author Di Serio, Francesco es_ES
dc.date.accessioned 2017-09-20T10:43:49Z
dc.date.available 2017-09-20T10:43:49Z
dc.date.issued 2012-06
dc.identifier.issn 0960-7412
dc.identifier.uri http://hdl.handle.net/10251/87625
dc.description.abstract [EN] How viroids, tiny non-protein-coding RNAs (250–400 nt), incite disease is unclear. One hypothesis is that viroid-derived small RNAs (vd-sRNAs; 21–24 nt) resulting from the host defensive response, via RNA silencing, may target for cleavage cell mRNAs and trigger a signal cascade, eventually leading to symptoms. Peach latent mosaic viroid (PLMVd), a chloroplast-replicating viroid, is particularly appropriate to tackle this question because it induces an albinism (peach calico, PC) strictly associated with variants containing a specific 12–14-nt hairpin insertion. By dissecting albino and green leaf sectors of Prunus persica (peach) seedlings inoculated with PLMVd natural and artificial variants, and cloning their progeny, we have established that the hairpin insertion sequence is involved in PC. Furthermore, using deep sequencing, semi-quantitative RT-PCR and RNA ligase-mediated rapid amplification of cDNA ends (RACE), we have determined that two PLMVd-sRNAs containing the PC-associated insertion (PC-sRNA8a and PC-sRNA8b) target for cleavage the mRNA encoding the chloroplastic heat-shock protein 90 (cHSP90), thus implicating RNA silencing in the modulation of host gene expression by a viroid. Chloroplast malformations previously reported in PC-expressing tissues are consistent with the downregulation of cHSP90, which participates in chloroplast biogenesis and plastid-tonucleus signal transduction in Arabidopsis. Besides PC-sRNA8a and PC-sRNA8b, both deriving from the lessabundant PLMVd ()) strand, we have identified other PLMVd-sRNAs potentially targeting peach mRNAs. These results also suggest that sRNAs derived from other PLMVd regions may downregulate additional peach genes, ultimately resulting in other symptoms or in a more favorable host environment for viroid infection. es_ES
dc.description.sponsorship This work was supported by a dedicated grant from the Italian Ministry of Economy and Finance to the CNR (Legge n. 191/2009), the Dipartimento Agroalimentare of the CNR of Italy (A. Leone and D. Mariotti 2008 award for advanced research in agriculture to FDS) and by the Ministerio de Ciencia e Innovacion of Spain (grants BFU2008-03154 and BFU2011-28443 to RF).
dc.language Inglés es_ES
dc.publisher Wiley es_ES
dc.relation MEF/191/2009 es_ES
dc.relation MICINN/BFU2008-03154 es_ES
dc.relation MICINN/BFU2011-28443
dc.relation.ispartof Plant Journal es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Chloroplast development es_ES
dc.subject Heat-shock protein 90 es_ES
dc.subject Non-coding RNAs es_ES
dc.subject PLMVd es_ES
dc.subject RNA silencing es_ES
dc.subject Viroid pathogenesis es_ES
dc.title Small RNAs containing the pathogenic determinant of a chloroplast-replicating viroid guide the degradation of a host mRNA as predicted by RNA silencing es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/j.1365-313X.2012.04940.x
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Navarro, B.; Gisel, A.; Rodio, ME.; Delgado Villar, SG.; Flores Pedauye, R.; Di Serio, F. (2012). Small RNAs containing the pathogenic determinant of a chloroplast-replicating viroid guide the degradation of a host mRNA as predicted by RNA silencing. Plant Journal. 70(6):991-1003. doi:10.1111/j.1365-313X.2012.04940.x es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://doi.org/10.1111/j.1365-313X.2012.04940.x es_ES
dc.description.upvformatpinicio 991 es_ES
dc.description.upvformatpfin 1003 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 70 es_ES
dc.description.issue 6 es_ES
dc.relation.senia 233152 es_ES
dc.identifier.pmid 22332758
dc.contributor.funder Ministerio de Ciencia e Innovación
dc.contributor.funder Ministero dell'Economia e Finanze, Italia


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