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dc.contributor.author | Shi, Jian Xin![]() |
es_ES |
dc.contributor.author | Adato, Avital![]() |
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dc.contributor.author | Alkan, Noam![]() |
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dc.contributor.author | He, Yonghua![]() |
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dc.contributor.author | Lashbrooke, Justin![]() |
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dc.contributor.author | Matas, Antonio J.![]() |
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dc.contributor.author | Meir, Sagit![]() |
es_ES |
dc.contributor.author | Malitsky, Sergey![]() |
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dc.contributor.author | Isaacson, Tal![]() |
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dc.contributor.author | Prusky, Dov![]() |
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dc.contributor.author | Leshkowitz, Dena![]() |
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dc.contributor.author | Schreiber, Lukas![]() |
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dc.contributor.author | Granell Richart, Antonio![]() |
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dc.contributor.author | Widemann, Emilie![]() |
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dc.contributor.author | Grausem, Bernard![]() |
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dc.date.accessioned | 2017-09-21T07:05:27Z | |
dc.date.available | 2017-09-21T07:05:27Z | |
dc.date.issued | 2013-01 | |
dc.identifier.issn | 0028-646X | |
dc.identifier.uri | http://hdl.handle.net/10251/87698 | |
dc.description.abstract | [EN] Fleshy tomato fruit typically lacks stomata; therefore, a proper cuticle is particularly vital for fruit development and interaction with the surroundings. Here, we characterized the tomato SlSHINE3 (SlSHN3) transcription factor to extend our limited knowledge regarding the regulation of cuticle formation in fleshy fruits. We created SlSHN3 overexpressing and silenced plants, and used them for detailed analysis of cuticular lipid compositions, phenotypic characterization, and the study on the mode of SlSHN3 action. Heterologous expression of SlSHN3 in Arabidopsis phenocopied overexpression of the Arabidopsis SHNs. Silencing of SlSHN3 results in profound morphological alterations of the fruit epidermis and significant reduction in cuticular lipids. We demonstrated that SlSHN3 activity is mediated by control of genes associated with cutin metabolism and epidermal cell patterning. As with SlSHN3 RNAi lines, mutation in the SlSHN3 target gene, SlCYP86A69, resulted in severe cutin deficiency and altered fruit surface architecture. In vitro activity assays demonstrated that SlCYP86A69 possesses NADPH-dependent x-hydroxylation activity, particularly of C18:1 fatty acid to the 18-hydroxyoleic acid cutin monomer. This study provided insights into transcriptional mechanisms mediating fleshy fruit cuticle formation and highlighted the link between cutin metabolism and the process of fruit epidermal cell patterning. | es_ES |
dc.description.sponsorship | We thank Eugenia Klein and Eyal Shimoni for help with the electron microscopy assays, Dana Elyahu and Dorit Levy for technical assistance, and Ester Feldmesser for bioinformatics analyses. We thank David Nelson for naming of the CYP450s analyzed in this study and Laetitia Martin for careful reading of this manuscript. A. Aharoni is the incumbent of the Adolpho and Evelyn Blum Career Development Chair of Cancer Research. The work was supported by the Israel Science Foundation and the European Research Council (ERC) project SAMIT (FP7 program) to A.A., Agence Nationale de la Recherche (SUIT project: ANR-09-KBBE-006-001) to B.G., NSF Plant Genome Research Program (DBI-0606595), the United States-Israel Binational Agricultural Research and Development Fund (IS-4234-09), and CUAES-Hatch grant (NYC-18442) to J.K.C.R. All authors declare that there is no conflict of interest. | en_EN |
dc.language | Inglés | es_ES |
dc.publisher | Wiley | es_ES |
dc.relation.ispartof | New Phytologist | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Cutin | es_ES |
dc.subject | CYP86A69 | es_ES |
dc.subject | Epidermal cell patterning | es_ES |
dc.subject | Solanum lycopersicum | es_ES |
dc.subject | Surface architecture | es_ES |
dc.subject | Transcriptional regulation | es_ES |
dc.subject | Wax | es_ES |
dc.title | The tomato SISHINE3 transcription factor regulates fruit cuticle formation and epidermal patterning | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1111/nph.12032 | |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/204575/EU/Systems Analysis of Plant Metabolism through the Integration of Heterogeneous Data from Genetics, Informatics and Metabolomics/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ANR//ANR-09-KBBE-0006/FR/Engineer SUberin biopolyester production in potato to Improve stress Tolerance/SUIT/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/NSF//0606595/US/Characterization of the Tomato Secretome Using Integrated Functional and Computational Strategies/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/BARD//NYC-18442/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/CUAES//NYC-18442/ | |
dc.rights.accessRights | Cerrado | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Shi, JX.; Adato, A.; Alkan, N.; He, Y.; Lashbrooke, J.; Matas, AJ.; Meir, S.... (2013). The tomato SISHINE3 transcription factor regulates fruit cuticle formation and epidermal patterning. New Phytologist. 197(2):468-480. https://doi.org/10.1111/nph.12032 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://doi.org/10.1111/nph.12032 | es_ES |
dc.description.upvformatpinicio | 468 | es_ES |
dc.description.upvformatpfin | 480 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 197 | es_ES |
dc.description.issue | 2 | es_ES |
dc.relation.senia | 261398 | es_ES |
dc.identifier.pmid | 23205954 | |
dc.contributor.funder | European Commission | |
dc.contributor.funder | Agence Nationale de la Recherche, Francia | |
dc.contributor.funder | National Science Foundation, EEUU | |
dc.contributor.funder | U.S. - Israel Binational Agricultural Research and Development Fund | |
dc.contributor.funder | Israel Science Foundation | |
dc.contributor.funder | European Research Council | |
dc.contributor.funder | Cornell University Agricultural Experiment Station, EEUU | es_ES |