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Gelatin-Hyaluronic Acid Hydrogels with Tuned Stiffness to Counterbalance Cellular Forces and Promote Cell Differentiation

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Gelatin-Hyaluronic Acid Hydrogels with Tuned Stiffness to Counterbalance Cellular Forces and Promote Cell Differentiation

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dc.contributor.author Poveda Reyes, Sara es_ES
dc.contributor.author Moulisova, Vladimira es_ES
dc.contributor.author Sanmartín Masiá, Esther del Rosario es_ES
dc.contributor.author Quintanilla-Sierra, Luis es_ES
dc.contributor.author Salmerón Sánchez, Manuel es_ES
dc.contributor.author Gallego-Ferrer, Gloria es_ES
dc.date.accessioned 2017-09-29T11:59:49Z
dc.date.available 2017-09-29T11:59:49Z
dc.date.issued 2016-09
dc.identifier.issn 1616-5187
dc.identifier.uri http://hdl.handle.net/10251/88293
dc.description.abstract [EN] Cells interact mechanically with their environment, exerting mechanical forces that probe the extracellular matrix (ECM). The mechanical properties of the ECM determine cell behavior and control cell differentiation both in 2D and 3D environments. Gelatin (Gel) is a soft hydrogel into which cells can be embedded. This study shows significant 3D Gel shrinking due to the high traction cellular forces exerted by the cells on the matrix, which prevents cell differentiation. To modulate this process, Gel with hyaluronic acid (HA) has been combined in an injectable crosslinked hydrogel with controlled Gel-HA ratio. HA increases matrix stiffness. The addition of small amounts of HA leads to a significant reduction in hydrogel shrinking after cell encapsulation (C2C12 myoblasts). We show that hydrogel stiffness counterbalanced traction forces of cells and this was decisive in promoting cell differentiation and myotube formation of C2C12 encapsulated in the hybrid hydrogels. es_ES
dc.description.sponsorship The authors are grateful for the financial support received from the Spanish Ministry through the MAT2013-46467-C4-1-R project (including the FEDER financial support), the BES-2011-046144, and the EEBB-I-14-08725 grants. CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program. CIBER actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. M.S.S. acknowledges ERC through HealInSynergy 306990.
dc.language Inglés es_ES
dc.publisher Wiley es_ES
dc.relation.ispartof Macromolecular Bioscience es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Gelatin es_ES
dc.subject Hyaluronic acid es_ES
dc.subject Injectable hydrogels es_ES
dc.subject Matrix stiffness es_ES
dc.subject Myoblast differentiation es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.subject.classification TERMODINAMICA APLICADA (UPV) es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title Gelatin-Hyaluronic Acid Hydrogels with Tuned Stiffness to Counterbalance Cellular Forces and Promote Cell Differentiation es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1002/mabi.201500469
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//MAT2013-46467-C4-1-R/ES/ESTIMULACION MECANICA LOCAL DE CELULAS MESENQUIMALES DE CARA A SU DIFERENCIACION OSTEOGENICA Y CONDROGENICA EN MEDICINA REGENERATIVA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//EEBB-I-14-08725/ES/EEBB-I-14-08725/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BES-2011-046144/ES/BES-2011-046144/
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/306990/EU/Material-driven Fibronectin Fibrillogenesis to Engineer Synergistic Growth Factor Microenvironments/
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials es_ES
dc.description.bibliographicCitation Poveda Reyes, S.; Moulisova, V.; Sanmartín Masiá, EDR.; Quintanilla-Sierra, L.; Salmerón Sánchez, M.; Gallego-Ferrer, G. (2016). Gelatin-Hyaluronic Acid Hydrogels with Tuned Stiffness to Counterbalance Cellular Forces and Promote Cell Differentiation. Macromolecular Bioscience. 16(9):1311-1324. https://doi.org/10.1002/mabi.201500469 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi. org/10.1002/mabi.201500469 es_ES
dc.description.upvformatpinicio 1311 es_ES
dc.description.upvformatpfin 1324 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 16 es_ES
dc.description.issue 9 es_ES
dc.relation.senia 325688 es_ES
dc.identifier.eissn 1616-5195
dc.identifier.pmid 27213762
dc.contributor.funder Ministerio de Economía y Competitividad
dc.contributor.funder Instituto de Salud Carlos III
dc.contributor.funder European Regional Development Fund
dc.contributor.funder European Research Council
dc.contributor.funder Ministerio de Ciencia e Innovación


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