- -

Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity

RiuNet: Repositorio Institucional de la Universidad Politécnica de Valencia

Compartir/Enviar a

Citas

Estadísticas

  • Estadisticas de Uso

Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity

Mostrar el registro sencillo del ítem

Ficheros en el ítem

dc.contributor.author Vilariño Feltrer, Guillermo es_ES
dc.contributor.author Martínez Ramos, Cristina es_ES
dc.contributor.author Monleon De la Fuente, Adrian es_ES
dc.contributor.author Vallés Lluch, Ana es_ES
dc.contributor.author Moratal Pérez, David es_ES
dc.contributor.author Barcia Albacar, Juan Antonio es_ES
dc.contributor.author Monleón Pradas, Manuel es_ES
dc.date.accessioned 2017-10-18T11:07:57Z
dc.date.available 2017-10-18T11:07:57Z
dc.date.issued 2016 es_ES
dc.identifier.issn 1742-7061 es_ES
dc.identifier.uri http://hdl.handle.net/10251/89409
dc.description.abstract [EN] Cell transplantation therapies in the nervous system are frequently hampered by glial scarring and cell drain from the damaged site, among others. To improve this situation, new biomaterials may be of help. Here, novel single-channel tubular conduits based on hyaluronic acid (HA) with and without poly-l-lactide acid fibers in their lumen were fabricated. Rat Schwann cells were seeded within the conduits and cultured for 10days. The conduits possessed a three-layered porous structure that impeded the leakage of the cells seeded in their interior and made them impervious to cell invasion from the exterior, while allowing free transport of nutrients and other molecules needed for cell survival. The channel's surface acted as a template for the formation of a cylindrical sheath-like tapestry of Schwann cells continuously spanning the whole length of the lumen. Schwann-cell tubes having a diameter of around 0.5mm and variable lengths can thus be generated. This structure is not found in nature and represents a truly engineered tissue, the outcome of the specific cell-material interactions. The conduits might be useful to sustain and protect cells for transplantation, and the biohybrids here described, together with neuronal precursors, might be of help in building bridges across significant distances in the central and peripheral nervous system. es_ES
dc.description.sponsorship The authors acknowledge financing through projects MAT2011-28791-C03-02 and 03, and ERA-NET NEURON project PRI-PIMNEU-2011-1372. We thank the Cytomics Core Facility at Principe Felipe Research Center (CIPF, Valencia, Spain) for their support and advice in flow cytometry experiments, and the Electron Microscopy Service at the UPV, where the SEM images were obtained. The authors thankfully acknowledge the reviewers' comments, which have helped to improve the clarity of the paper's presentation. en_EN
dc.language Inglés es_ES
dc.relation.ispartof Acta Biomaterialia es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Nervous system regeneration es_ES
dc.subject Hyaluronic acid es_ES
dc.subject Schwann cell es_ES
dc.subject Protective barrier es_ES
dc.subject Cell sheet es_ES
dc.subject Electron Microscopy Service of the UPV
dc.subject.classification TECNOLOGIA ELECTRONICA es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.title Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.actbio.2015.10.040 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//MAT2011-28791-C03-02/ES/MATERIALES DE SOPORTE Y LIBERACION CONTROLADA PARA LA REGENERACION DE ESTRUCTURAS NEURALES AFECTADAS POR ICTUS/
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//PRI-PIMNEU-2011-1372/ES/MATERIALES BIFUNCIONALES PARA LA REGENERACION NEURAL DE AREAS AFECTADAS POR ICTUS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//MAT2011-28791-C03-03/ES/CONSTRUCTOS PARA LA REGENERACION GUIADA DE ESTRUCTURAS DEL SISTEMA NERVIOSO CENTRAL/
dc.rights.accessRights Abierto es_ES
dc.date.embargoEndDate 2018-01-15 es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Electrónica - Departament d'Enginyeria Electrònica es_ES
dc.description.bibliographicCitation Vilariño Feltrer, G.; Martínez Ramos, C.; Monleon De La Fuente, A.; Vallés Lluch, A.; Moratal Pérez, D.; Barcia Albacar, JA.; Monleón Pradas, M. (2016). Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity. Acta Biomaterialia. 30:199-211. https://doi.org/10.1016/j.actbio.2015.10.040 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.actbio.2015.10.040 es_ES
dc.description.upvformatpinicio 199 es_ES
dc.description.upvformatpfin 211 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 30 es_ES
dc.relation.pasarela S\299775 es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación


Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem