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Evaluation of PCR and non-radioactive molecular hybridization techniques for the routine diagnosis of Tomato leaf curl new delhi virus, Tomato yellow leaf curl virus and tomato yellow leaf curl Sardinia virus

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Evaluation of PCR and non-radioactive molecular hybridization techniques for the routine diagnosis of Tomato leaf curl new delhi virus, Tomato yellow leaf curl virus and tomato yellow leaf curl Sardinia virus

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Alfaro Fernández, AO.; Sanchez Navarro, JA.; Landeira, M.; Font San Ambrosio, MI.; Hernández Llopis, D.; Pallás Benet, V. (2016). Evaluation of PCR and non-radioactive molecular hybridization techniques for the routine diagnosis of Tomato leaf curl new delhi virus, Tomato yellow leaf curl virus and tomato yellow leaf curl Sardinia virus. JOURNAL OF PLANT PATHOLOGY. 98(2):245-254. doi:10.4454/JPP.V98I2.003

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Title: Evaluation of PCR and non-radioactive molecular hybridization techniques for the routine diagnosis of Tomato leaf curl new delhi virus, Tomato yellow leaf curl virus and tomato yellow leaf curl Sardinia virus
Author:
UPV Unit: Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani
Universitat Politècnica de València. Departamento de Ecosistemas Agroforestales - Departament d'Ecosistemes Agroforestals
Issued date:
Abstract:
[EN] The begomovirus Tomato leaf curl New Delhi virus (ToLCNDV) has been reported as a causal agent of leaf curl disease in tomato and other solanaceous crops and, more recently, affecting different cucurbitaceous crops. ...[+]
Subjects: Molecular detection methods , Dig-RNA probe , Begomovirus , Routine diagnosis
Copyrigths: Cerrado
Source:
JOURNAL OF PLANT PATHOLOGY. (issn: 1125-4653 )
DOI: 10.4454/JPP.V98I2.003
Publisher:
EDIZIONE ETS, PISA, ITALY
Publisher version: http://dx.doi.org/10.4454/JPP.V98I2.003
Thanks:
We thank L. Corachan for her excellent technical assistance. This work was supported by grants BIO2014-54862-R from the Spanish granting agency DGICYT and SP20140770 funded by Spanish Ministry of Education, Culture and ...[+]
Type: Artículo

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