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Non-invasive and simple methods for sampling rabbit DNA for PCR analysis of melanocortin 1 receptor (MC1R) gene mutations: a technical note

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Non-invasive and simple methods for sampling rabbit DNA for PCR analysis of melanocortin 1 receptor (MC1R) gene mutations: a technical note

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dc.contributor.author Fontanesi, L.
dc.contributor.author Tazzoli, M.
dc.contributor.author Russo, V.
dc.date.accessioned 2011-02-08T10:55:50Z
dc.date.available 2011-02-08T10:55:50Z
dc.date.issued 2007
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/9480
dc.description.abstract [EN]Simple and non-invasive methods of collecting and preserving biological specimens for genetic analysis can be useful in reducing the discomfort of the involved animals and can be applicable for highthroughput studies that require a large number of biosamples. In order to have the possibility to analyse a large number of samples and making easier the collection of rabbit biological materials as well as to reduce the cost and time of the genotyping procedures, here we tested different protocols for the genotyping of mutations identified in the rabbit melanocortin receptor 1 (MC1R) gene that are associated to coat colours in different rabbit breeds. These protocols make use of rabbit hair roots or cell preparations from buccal swabs with a direct PCR amplification without previous DNA extraction. Hair samples were collected from 60 rabbits of different breeds and preserved for up to 12 months at 4°C. For each hair sample 5-10 hair roots were clipped a few mm from the root directly within a 0.2 ml PCR tube. Buccal cells were collected from 10 crossbred and purebred rabbits and prepared after a few steps for PCR. The PCR fragments were analysed by means of polyacrylamide gel electrophoresis and stained with ethidium bromide or using fluorescent labelled products resolved in a capillary automatic sequencer. The success genotyping rate of the hair and buccal cell samples was 98% and 80%, respectively. These values are comparable to the success rates of other protocols described in human or other animals involving previous DNA extraction. The obtained results show that noninvasive methods of biological specimen collection that make it possible to analyse the DNA without previous extraction can be easily and routinely applied in rabbit molecular genetic studies. However, due to the higher success rate and the easier method of collection, plucked hairs seem the best source for high-throughput biosampling in this species. es_ES
dc.description.sponsorship This work was supported by RFO funds from the University of Bologna. We thank several rabbit breeders and Associazione Nazionale Coniglicoltori Italiani (ANCI) for their collaboration in the sampling of biological materials
dc.language Inglés es_ES
dc.publisher World Rabbit Science. ICTA. UPV es_ES
dc.relation.ispartof World Rabbit Science
dc.rights Reconocimiento - No comercial (by-nc) es_ES
dc.subject Rabbit es_ES
dc.subject Hair dna es_ES
dc.subject Buccal swabs es_ES
dc.subject Biological specimens es_ES
dc.subject Direct pcr es_ES
dc.subject Mc1r mutations es_ES
dc.title Non-invasive and simple methods for sampling rabbit DNA for PCR analysis of melanocortin 1 receptor (MC1R) gene mutations: a technical note es_ES
dc.type Artículo es_ES
dc.date.updated 2011-02-08T10:10:53Z
dc.identifier.doi 10.4995/wrs.2007.598
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Fontanesi, L.; Tazzoli, M.; Russo, V. (2007). Non-invasive and simple methods for sampling rabbit DNA for PCR analysis of melanocortin 1 receptor (MC1R) gene mutations: a technical note. World Rabbit Science. 15(2). doi:10.4995/wrs.2007.598 es_ES
dc.relation.publisherversion https://doi.org/10.4995/wrs.2007.598
dc.description.volume 15
dc.description.issue 2
dc.identifier.eissn 1989-8886 es_ES
dc.contributor.funder Universitat de Bologna


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