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Effective generation of genetically modified rabbits by sperm mediated gene transfer

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Effective generation of genetically modified rabbits by sperm mediated gene transfer

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dc.contributor.author Vasicek, D.
dc.contributor.author Vasickova, K.
dc.contributor.author Parkanyi, V.
dc.contributor.author Rafay, J.
dc.contributor.author Ondruska, L.
dc.date.accessioned 2011-02-08T12:07:15Z
dc.date.available 2011-02-08T12:07:15Z
dc.date.issued 2007
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/9486
dc.description.abstract [EN] Many reports in the past decade showing successful sperm mediated gene transfer (SMGT) offoreign DNA into both non-mammalian and mammalian animals. Fresh collected, extender washed rabbit spermcells were co-cultured with linear 14.3 kb gene construct and used for AI. Transgene integration in rabbitgenome was verified by PCR analysis. The PCR results show that transgenic founder rabbits transmittedtransgene to F1 generation. Our preliminary results suggest that SMGT could be an effective method forproduction of genetically modified rabbits. es_ES
dc.description.sponsorship We would like to thank Mr. Jan Pecho and Dipl. Ing. Ivan Chlebec for taking care of the animals and for providing technical assistance. This work was financially supported by Ministry of Agriculture Slovak Republic and Slovak Research and Development Agency under the contract No. APVV-27-005505.
dc.language Inglés es_ES
dc.publisher World Rabbit Science. ICTA. UPV es_ES
dc.relation.ispartof World Rabbit Science
dc.rights Reserva de todos los derechos es_ES
dc.subject Transgenic rabbit es_ES
dc.subject Sperm mediated gene transfer (smgt) es_ES
dc.subject Human factor viii es_ES
dc.title Effective generation of genetically modified rabbits by sperm mediated gene transfer es_ES
dc.type Artículo es_ES
dc.date.updated 2011-02-08T11:13:25Z
dc.identifier.doi 10.4995/wrs.2007.593
dc.relation.projectID info:eu-repo/grantAgreement/SRDA//APVV-27-005505/
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Vasicek, D.; Vasickova, K.; Parkanyi, V.; Rafay, J.; Ondruska, L. (2007). Effective generation of genetically modified rabbits by sperm mediated gene transfer. World Rabbit Science. 15(3). https://doi.org/10.4995/wrs.2007.593 es_ES
dc.description.accrualMethod SWORD es_ES
dc.relation.publisherversion https://doi.org/10.4995/wrs.2007.593
dc.description.volume 15
dc.description.issue 3
dc.identifier.eissn 1989-8886 es_ES
dc.contributor.funder Slovak Research and Development Agency


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