- -

Does the inclusion of protease inhibitors in the insemination extender affect rabbit reproductive performance?

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

  • Estadisticas de Uso

Does the inclusion of protease inhibitors in the insemination extender affect rabbit reproductive performance?

Show simple item record

Files in this item

dc.contributor.author Casares-Crespo, Lucía es_ES
dc.contributor.author Vicente Antón, José Salvador es_ES
dc.contributor.author Talavan, A.M. es_ES
dc.contributor.author Viudes De Castro, María Pilar es_ES
dc.date.accessioned 2018-02-01T07:59:37Z
dc.date.available 2018-02-01T07:59:37Z
dc.date.issued 2016 es_ES
dc.identifier.issn 0093-691X es_ES
dc.identifier.uri http://hdl.handle.net/10251/96366
dc.description.abstract [EN] The bioavailability of buserelin acetate when added to the seminal dose appears to be determined by the activity of the existing aminopeptidases. Thus, the addition of amino peptidase inhibitors to rabbit semen extenders could be a solution to decrease the hormone degradation. This study was conducted to evaluate the effect of the protease activity inhibition on rabbit semen quality parameters and reproductive performance after artificial insemination. Seminal quality was not affected by the incubation with protease inhibitors, being the values of motility, viability, and acrosome integrity not significantly different between the protease inhibitors and the control group. In addition, seminal plasma aminopeptidase activity was inhibited in a 55.1% by the protease inhibitors. On the other hand, regarding the effect of protease inhibitors on reproductive performance, our results showed that the presence of protease inhibitors affected the prolificacy rate (9.2 +/- 0.26 and 9.3 +/- 0.23 vs. 8.2 +/- 0.22 total born per litter for negative control, positive control, and aminopeptidase inhibitors group, respectively; P < 0.05), having this group one kit less per delivery. We conclude that the addition of a wide variety of protease inhibitors in the rabbit semen extender negatively affects prolificacy rate. Therefore, the development of new extenders with specific aminopeptidase inhibitors would be one of the strategies to increase the bioavailability of GnRH analogues without affecting the litter size. (C) 2016 Elsevier Inc. All rights reserved. es_ES
dc.description.sponsorship This research was supported in part by the RTA2013-00058-00-00 from INIA, the European Social Fund and the European FEDER Funds. L Casares-Crespo is supported by a scholarship from Institute Valenciano de Investigaciones Agrarias (IVIA) and the European Social Fund. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Theriogenology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Rabbit es_ES
dc.subject Aminopeptidase activity es_ES
dc.subject Protease inhibitor es_ES
dc.subject Seminal quality es_ES
dc.subject Reproductive performance es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Does the inclusion of protease inhibitors in the insemination extender affect rabbit reproductive performance? es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.theriogenology.2015.10.044 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//RTA2013-00058-00-00/ES/Estudio de la inducción de la ovulación en conejas. Desarrollo de diluyentes para inseminacion artificial en cunicultura./
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Casares-Crespo, L.; Vicente Antón, JS.; Talavan, A.; Viudes De Castro, MP. (2016). Does the inclusion of protease inhibitors in the insemination extender affect rabbit reproductive performance?. Theriogenology. 85(5):928-932. doi:10.1016/j.theriogenology.2015.10.044 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.theriogenology.2015.10.044 es_ES
dc.description.upvformatpinicio 928 es_ES
dc.description.upvformatpfin 932 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 85 es_ES
dc.description.issue 5 es_ES
dc.relation.pasarela S\300873 es_ES
dc.contributor.funder Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
dc.contributor.funder Ministerio de Economía y Competitividad
dc.contributor.funder European Regional Development Fund
dc.contributor.funder European Social Fund


This item appears in the following Collection(s)

Show simple item record