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Use of embryos extracted from individual Cannabis sativa seeds for genetic studies and forensic applications

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Use of embryos extracted from individual Cannabis sativa seeds for genetic studies and forensic applications

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dc.contributor.author Soler Aleixandre, Salvador es_ES
dc.contributor.author Borràs Palomares, Dionís es_ES
dc.contributor.author Vilanova Navarro, Santiago es_ES
dc.contributor.author Sifres Cuerda, Alicia Gemma es_ES
dc.contributor.author Andújar, Isabel es_ES
dc.contributor.author Figás-Moreno, María Del Rosario es_ES
dc.contributor.author Llosa, Ernesto es_ES
dc.contributor.author Prohens Tomás, Jaime es_ES
dc.date.accessioned 2018-02-06T08:16:41Z
dc.date.available 2018-02-06T08:16:41Z
dc.date.issued 2016 es_ES
dc.identifier.issn 0022-1198 es_ES
dc.identifier.uri http://hdl.handle.net/10251/96965
dc.description.abstract [EN] Legal limits on the psychoactive tetrahydrocannabinol (THC) content in Cannabis sativa plants have complicated genetic and forensic studies in this species. However, Cannabis seeds present very low THC levels. We developed a method for embryo extraction from seeds and an improved protocol for DNA extraction and tested this method in four hemp and six marijuana varieties. This embryo extraction method enabled the recovery of diploid embryos from individual seeds. An improved DNA extraction protocol (CTAB3) was used to obtain DNA from individual embryos at a concentration and quality similar to DNA extracted from leaves. DNA extracted from embryos was used for SSR molecular characterization in individuals from the 10 varieties. A unique molecular profile for each individual was obtained, and a clear differentiation between hemp and marijuana varieties was observed. The combined embryo extraction-DNA extraction methodology and the new highly polymorphic SSR markers facilitate genetic and forensic studies in Cannabis. es_ES
dc.language Inglés es_ES
dc.publisher Blackwell Publishing es_ES
dc.relation.ispartof Journal of Forensic Sciences es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Forensic science es_ES
dc.subject Cannabis sativa es_ES
dc.subject Marijuana es_ES
dc.subject Seed embryo es_ES
dc.subject DNA extraction es_ES
dc.subject Simple sequence repeats es_ES
dc.subject.classification GENETICA es_ES
dc.title Use of embryos extracted from individual Cannabis sativa seeds for genetic studies and forensic applications es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/1556-4029.12995 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.description.bibliographicCitation Soler Aleixandre, S.; Borràs Palomares, D.; Vilanova Navarro, S.; Sifres Cuerda, AG.; Andújar, I.; Figás-Moreno, MDR.; Llosa, E.... (2016). Use of embryos extracted from individual Cannabis sativa seeds for genetic studies and forensic applications. Journal of Forensic Sciences. 61(2):494-500. doi:10.1111/1556-4029.12995 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1111/1556-4029.12995 es_ES
dc.description.upvformatpinicio 494 es_ES
dc.description.upvformatpfin 500 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 61 es_ES
dc.description.issue 2 es_ES
dc.identifier.pmid 27404624
dc.relation.pasarela S\329569 es_ES


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