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A comparison of techniques for studying oogenesis in the European eel Anguilla anguilla

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A comparison of techniques for studying oogenesis in the European eel Anguilla anguilla

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dc.contributor.author Mazzeo ., Ilaria es_ES
dc.contributor.author Giorgini, E. es_ES
dc.contributor.author Gioacchini, G. es_ES
dc.contributor.author Maradonna, F. es_ES
dc.contributor.author Vilchez Olivencia, Maria Carmen es_ES
dc.contributor.author Baloche, S. es_ES
dc.contributor.author Dufour, S. es_ES
dc.contributor.author Pérez Igualada, Luz María es_ES
dc.contributor.author Carnevali, O. es_ES
dc.contributor.author Asturiano Nemesio, Juan Francisco es_ES
dc.date.accessioned 2018-02-23T05:21:31Z
dc.date.available 2018-02-23T05:21:31Z
dc.date.issued 2016 es_ES
dc.identifier.issn 0022-1112 es_ES
dc.identifier.uri http://hdl.handle.net/10251/98351
dc.description.abstract [EN] A multi-technique approach was used to study the changes occurring in European eel Anguilla anguilla ovaries during hormonally-induced vitellogenesis. Aside from classic techniques used to monitor the vitellogenic process, such as ovary histology, fat content analysis, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and vitellogenin enzyme linked immunosorbent assay (ELISA), a new technique, Fourier-transform infrared (FT-IR) microspectroscopy, was used to analyse A. anguilla ovaries. The results from the different techniques provided different ways of approaching the same process. Although it is considered a time consuming approach, of all the employed techniques, histology provided the most direct evidences about vitellogenesis. SDS-PAGE and ELISA were also useful for studying vitellogenesis, whereas fat analysis cannot be used for this purpose. The FT-IR analysis provided a representative IR spectrum for each ovarian stage (previtellogenic stage, early vitellogenic stage, mid-vitellogenic stage and late vitellogenic stage), demonstrating that it is a valid method able to illustrate the distribution of the oocytes within the ovary slices. The chemical maps obtained confirmed changes in lipid concentrations and revealed their distribution within the oocytes at different maturational stages. When the results and the accuracy of the FT-IR analysis were compared with those of the traditional techniques commonly used to establish the vitellogenic stage, it became evident that FT-IR is a useful and reliable tool, with many advantages, including the fact that it requires little biological material, the costs involved are low, analysis times are short and last but not least, the fact that it offers the possibility of simultaneously analysing various biocomponents of the same oocyte. (C) 2016 The Fisheries Society of the British Isles es_ES
dc.description.sponsorship This study was funded by the European Community's 7th Framework Programme under the Theme 2, Food, Agriculture and Fisheries and Biotechnology, grant agreement number 245257 (PRO-EEL) and the COST Office (COST Action FA1205: AQUAGAMETE). I.M. had a pre-doctoral grant from Generalitat Valenciana
dc.language Inglés es_ES
dc.publisher Blackwell Publishing es_ES
dc.relation CEE/CGA-FA1205-1 es_ES
dc.relation COMISION DE LAS COMUNIDADES EUROPEA/245257 es_ES
dc.relation.ispartof Journal of Fish Biology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject ELISA es_ES
dc.subject Folch es_ES
dc.subject FT-IR es_ES
dc.subject Ovary es_ES
dc.subject SDS-PAGE es_ES
dc.subject Vitellogenesis es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title A comparison of techniques for studying oogenesis in the European eel Anguilla anguilla es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/jfb.13103 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Mazzeo ., I.; Giorgini, E.; Gioacchini, G.; Maradonna, F.; Vilchez Olivencia, MC.; Baloche, S.; Dufour, S.... (2016). A comparison of techniques for studying oogenesis in the European eel Anguilla anguilla. Journal of Fish Biology. 89(4):2055-2069. doi:10.1111/jfb.13103 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1111/jfb.13103 es_ES
dc.description.upvformatpinicio 2055 es_ES
dc.description.upvformatpfin 2069 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 89 es_ES
dc.description.issue 4 es_ES
dc.identifier.pmid 27500665
dc.relation.pasarela S\315804 es_ES
dc.contributor.funder European Commission es_ES
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