- -

Optimization of a label-free biosensor vertically characterized based on a periodic lattice of high aspect ratio SU-8 nano-pillars with a simplified 2D theoretical model

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

Optimization of a label-free biosensor vertically characterized based on a periodic lattice of high aspect ratio SU-8 nano-pillars with a simplified 2D theoretical model

Show simple item record

Files in this item

dc.contributor.author Casquel Del Campo, Rafael es_ES
dc.contributor.author Holgado, Miguel es_ES
dc.contributor.author Sanza, F.J. es_ES
dc.contributor.author LAGUNA, MARI FE es_ES
dc.contributor.author BARRIOS, C.A. es_ES
dc.contributor.author López-Romero, D. es_ES
dc.contributor.author Ortega Higueruelo, Francisco José es_ES
dc.contributor.author Bañuls Polo, María-José es_ES
dc.contributor.author Puchades, Rosa es_ES
dc.contributor.author Maquieira Catala, Ángel es_ES
dc.date.accessioned 2018-03-01T05:27:14Z
dc.date.available 2018-03-01T05:27:14Z
dc.date.issued 2011 es_ES
dc.identifier.issn 1610-1634 es_ES
dc.identifier.uri http://hdl.handle.net/10251/98653
dc.description.abstract [EN] We have recently demonstrated a biosensor based on a lattice of SU8 pillars on a 1 ¿m SiO 2/Si wafer by measuring vertically reflectivity as a function of wavelength. The biodetection has been proven with the combination of Bovine Serum Albumin (BSA) protein and its antibody (antiBSA). A BSA layer is attached to the pillars; the biorecognition of antiBSA involves a shift in the reflectivity curve, related with the concentration of antiBSA. A detection limit in the order of 2 ng/ml is achieved for a rhombic lattice of pillars with a lattice parameter (a) of 800 nm, a height (h) of 420 nm and a diameter(d) of 200 nm. These results correlate with calculations using 3D-finite difference time domain method. A 2D simplified model is proposed, consisting of a multilayer model where the pillars are turned into a 420 nm layer with an effective refractive index obtained by using Beam Propagation Method (BPM) algorithm. Results provided by this model are in good correlation with experimental data, reaching a reduction in time from one day to 15 minutes, giving a fast but accurate tool to optimize the design and maximizing sensitivity, and allows analyzing the influence of different variables (diameter, height and lattice parameter). Sensitivity is obtained for a variety of configurations, reaching a limit of detection under 1 ng/ml. Optimum design is not only chosen because of its sensitivity but also its feasibility, both from fabrication (limited by aspect ratio and proximity of the pillars) and fluidic point of view. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. es_ES
dc.language Inglés es_ES
dc.publisher John Wiley & Sons es_ES
dc.relation.ispartof physica status solidi (c) es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Label free biosensor es_ES
dc.subject Optimization es_ES
dc.subject Simplified calculation es_ES
dc.subject Biodetection es_ES
dc.subject Biorecognition es_ES
dc.subject Bovine serum albumins es_ES
dc.subject Detection limits es_ES
dc.subject Effective refractive index es_ES
dc.subject Experimental data es_ES
dc.subject Good correlations es_ES
dc.subject High aspect ratio es_ES
dc.subject Label-free biosensor es_ES
dc.subject Lattice parameters es_ES
dc.subject Limit of detection es_ES
dc.subject Multilayer models es_ES
dc.subject Optimum designs es_ES
dc.subject Periodic lattices es_ES
dc.subject Reflectivity curve es_ES
dc.subject Rhombic lattices es_ES
dc.subject Si wafer es_ES
dc.subject Simplified calculations es_ES
dc.subject Simplified models es_ES
dc.subject Theoretical models es_ES
dc.subject Aspect ratio es_ES
dc.subject Beam propagation method es_ES
dc.subject Body fluids es_ES
dc.subject Finite difference time domain method es_ES
dc.subject Lattice constants es_ES
dc.subject Reflection es_ES
dc.subject Refractive index es_ES
dc.subject Semiconducting silicon compounds es_ES
dc.subject Silicon wafers es_ES
dc.subject Biosensors es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Optimization of a label-free biosensor vertically characterized based on a periodic lattice of high aspect ratio SU-8 nano-pillars with a simplified 2D theoretical model es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1002/pssc.201000412 es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Tecnología Nanofotónica - Institut Universitari de Tecnologia Nanofotònica es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic es_ES
dc.description.bibliographicCitation Casquel Del Campo, R.; Holgado, M.; Sanza, F.; Laguna, MF.; Barrios, C.; López-Romero, D.; Ortega Higueruelo, FJ.... (2011). Optimization of a label-free biosensor vertically characterized based on a periodic lattice of high aspect ratio SU-8 nano-pillars with a simplified 2D theoretical model. physica status solidi (c). 8(3):1-6. doi:10.1002/pssc.201000412 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1002/pssc.201000412 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 6 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 8 es_ES
dc.description.issue 3 es_ES
dc.relation.pasarela 212785 es_ES


This item appears in the following Collection(s)

Show simple item record