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Regulation of the Yeast Hxt6 Hexose Transporter by the Rod1 α-Arrestin, the Snf1 Protein Kinase, and the Bmh2 14-3-3 Protein

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Regulation of the Yeast Hxt6 Hexose Transporter by the Rod1 α-Arrestin, the Snf1 Protein Kinase, and the Bmh2 14-3-3 Protein

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dc.contributor.author Llopis Torregrosa, Vicent es_ES
dc.contributor.author Ferri-Blazquez, Alba es_ES
dc.contributor.author Adam-Artigues, Anna es_ES
dc.contributor.author Deffontaines, Emilie es_ES
dc.contributor.author van Heusden, G. Paul H. es_ES
dc.contributor.author Yenush, Lynne es_ES
dc.date.accessioned 2018-03-11T05:07:37Z
dc.date.available 2018-03-11T05:07:37Z
dc.date.issued 2016 es_ES
dc.identifier.issn 0021-9258 es_ES
dc.identifier.uri http://hdl.handle.net/10251/99146
dc.description.abstract [EN] Cell viability requires adaptation to changing environmental conditions. Ubiquitin-mediated endocytosis plays a crucial role in this process, because it provides a mechanism to remove transport proteins from the membrane. Arrestin-related trafficking proteins are important regulators of the endocytic pathway in yeast, facilitating selective ubiquitylation of target proteins by the E3 ubiquitin ligase, Rsp5. Specifically, Rod1 (Art4) has been reported to regulate the endocytosis of both the Hxt1, Hxt3, and Hxt6 glucose transporters and the Jen1 lactate transporter. Also, the AMP kinase homologue, Snf1, and 14-3-3 proteins have been shown to regulate Jen1 via Rod1. Here, we further characterized the role of Rod1, Snf1, and 14-3-3 in the signal transduction route involved in the endocytic regulation of the Hxt6 high affinity glucose transporter by showing that Snf1 interacts specifically with Rod1 and Rog3 (Art7), that the interaction between the Bmh2 and several arrestin-related trafficking proteins may be modulated by carbon source, and that both the 14-3-3 protein Bmh2 and the Snf1 regulatory domain interact with the arrestin-like domain containing the N-terminal half of Rod1 (amino acids 1-395). Finally, using both co-immunoprecipitation and bimolecular fluorescence complementation, we demonstrated the interaction of Rod1 with Hxt6 and showed that the localization of the Rod1-Hxt6 complex at the plasma membrane is affected by carbon source and is reduced upon overexpression of SNF1 and BMH2. es_ES
dc.description.sponsorship Supported by a predoctoral fellowship from the Polytechnic University of Valencia. en_EN
dc.language Inglés es_ES
dc.publisher American Society for Biochemistry and Molecular Biology es_ES
dc.relation.ispartof Journal of Biological Chemistry es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject 14-3-3 protein es_ES
dc.subject AMP-activated kinase (AMPK) es_ES
dc.subject Arrestin es_ES
dc.subject Membrane transport es_ES
dc.subject Trafficking
dc.title Regulation of the Yeast Hxt6 Hexose Transporter by the Rod1 α-Arrestin, the Snf1 Protein Kinase, and the Bmh2 14-3-3 Protein
dc.type Artículo
dc.identifier.doi 10.1074/jbc.M116.733923 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2011-30197-C03-03/ES/PAPEL DEL TRAFICO DE PROTEINAS EN LA HOMEOSTASIS DE IONES Y NUTRIENTES EN LEVADURA Y PLANTAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/J4NWO//2300160955/NL/
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Llopis Torregrosa, V.; Ferri-Blazquez, A.; Adam-Artigues, A.; Deffontaines, E.; Van Heusden, GPH.; Yenush, L. (2016). Regulation of the Yeast Hxt6 Hexose Transporter by the Rod1 α-Arrestin, the Snf1 Protein Kinase, and the Bmh2 14-3-3 Protein. Journal of Biological Chemistry. 291(29):14973-14985. https://doi.org/10.1074/jbc.M116.733923 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1074/jbc.M116.733923 es_ES
dc.description.upvformatpinicio 14973 es_ES
dc.description.upvformatpfin 14985 es_ES
dc.description.volume 291 es_ES
dc.description.issue 29 es_ES
dc.relation.pasarela S\320610 es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
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