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A Mycoplasma Genomic DNA Probe using Gated Nanoporous Anodic Alumina

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A Mycoplasma Genomic DNA Probe using Gated Nanoporous Anodic Alumina

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dc.contributor.author Pla, Luis es_ES
dc.contributor.author Xifre Perez, Elisabet es_ES
dc.contributor.author Ribes, Àngela es_ES
dc.contributor.author Aznar, Elena es_ES
dc.contributor.author Marcos Martínez, María Dolores es_ES
dc.contributor.author Marsal, L.F. es_ES
dc.contributor.author Martínez-Máñez, Ramón es_ES
dc.contributor.author Sancenón Galarza, Félix es_ES
dc.date.accessioned 2018-03-23T13:13:45Z
dc.date.available 2018-03-23T13:13:45Z
dc.date.issued 2017 es_ES
dc.identifier.issn 2192-6506 es_ES
dc.identifier.uri http://hdl.handle.net/10251/99654
dc.description.abstract [EN] A nanoporous anodic alumina (NAA)-based sensor system for the detection of Mycoplasma was developed through the implementation of "molecular gates" selective to the presence of this bacterium. The capped support showed a negligible cargo release, while presence of Mycoplasma genomic DNA resulted in the release of rhodamine B fluorescent dye. This sensor system presents a limit of detection of 20 genomic DNA copies mu L-1 and was applied to the detection of Mycoplasma bacteria in competitive environments, such as culture cell media. es_ES
dc.description.sponsorship We thank the Spanish Government (projects MAT2015-64139-C41-R, AGL2015-70235-C2-2-R, and TEC2015-71324-R (MINECO/FEDER, UE)), the Generalitat Valenciana (project PROMETEOII/2014/047), the Catalan authority (project AGAUR 2014SGR1344), and ICREA under the 2014 ICREA Academia Award for support. L.P. thanks to PROMETEOII/2014/047 for his contract. We thank the Electron Microscopy Service at the UPV for support. en_EN
dc.language Inglés es_ES
dc.publisher John Wiley & Sons es_ES
dc.relation MINECO/AGL2015-70235-C2-2-R es_ES
dc.relation MINECO/MAT2015-64139-C4-1-R es_ES
dc.relation GV/PROMETEOII/2014/047 es_ES
dc.relation MINECO/TEC2015-71324-R
dc.relation GC/AGAUR 2014SGR1344
dc.relation.ispartof ChemPlusChem es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject DNA es_ES
dc.subject Gated materials es_ES
dc.subject Mycoplasma detection es_ES
dc.subject Nanoporous anodic alumina es_ES
dc.subject Electron Microscopy Service of the UPV
dc.subject.classification INGENIERIA DE LA CONSTRUCCION es_ES
dc.subject.classification QUIMICA INORGANICA es_ES
dc.subject.classification QUIMICA ORGANICA es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title A Mycoplasma Genomic DNA Probe using Gated Nanoporous Anodic Alumina es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1002/cplu.201600651 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro de Tecnologías Físicas: Acústica, Materiales y Astrofísica - Centre de Tecnologies Físiques: Acústica, Materials i Astrofísica es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Pla, L.; Xifre Perez, E.; Ribes, À.; Aznar, E.; Marcos Martínez, MD.; Marsal, L.; Martínez-Máñez, R.... (2017). A Mycoplasma Genomic DNA Probe using Gated Nanoporous Anodic Alumina. ChemPlusChem. 82(3):337-341. doi:10.1002/cplu.201600651 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1002/cplu.201600651 es_ES
dc.description.upvformatpinicio 337 es_ES
dc.description.upvformatpfin 341 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 82 es_ES
dc.description.issue 3 es_ES
dc.relation.pasarela S\336662 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Ministerio de Economía, Industria y Competitividad es_ES
dc.contributor.funder Generalitat de Catalunya


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