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Repression of ergosterol biosynthesis is essential for stress resistance and is mediated by the Hog1 MAP kinase and the Mot3 and Rox1 transcription factors

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Repression of ergosterol biosynthesis is essential for stress resistance and is mediated by the Hog1 MAP kinase and the Mot3 and Rox1 transcription factors

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dc.contributor.author Martínez Montañés, Fernando Vicente es_ES
dc.contributor.author Pascual-Ahuir Giner, María Desamparados es_ES
dc.contributor.author Proft ., Markus Hans es_ES
dc.date.accessioned 2018-03-23T13:52:46Z
dc.date.available 2018-03-23T13:52:46Z
dc.date.issued 2010 es_ES
dc.identifier.issn 0950-382X es_ES
dc.identifier.uri http://hdl.handle.net/10251/99673
dc.description.abstract [EN] Hyperosmotic stress triggers a complex adaptive response that is dominantly regulated by the Hog1 MAP kinase in yeast. Here we characterize a novel physiological determinant of osmostress tolerance, which involves the Hog1-dependent transcriptional downregulation of ergosterol biosynthesis genes (ERG). Yeast cells considerably lower their sterol content in response to high osmolarity. The transcriptional repressors Mot3 and Rox1 are essential for this response. Both factors together with Hog1 are required to rapidly and transiently shut down transcription of ERG2 and ERG11 upon osmoshock. Mot3 abundance and its binding to the ERG2 promoter is stimulated by osmostress in a Hog1-dependent manner. As an additional layer of control, the expression of the main transcriptional activator of ERG gene expression, Ecm22, is negatively regulated by Hog1 and Mot3/Rox1 upon salt shock. Oxidative stress also triggers repression of ERG2, 11 transcription and a profound decrease in total sterol levels. However, this response was only partially dependent on Mot3/Rox1 and Hog1. Finally, we show that the upc2-1 mutation confers stress insensitive hyperaccumulation of ergosterol, overexpression of ERG2, 11 and severe sensitivity to salt and oxidative stress. Our results indicate that transcriptional control of ergosterol biosynthesis is an important physiological target of stress signalling. es_ES
dc.description.sponsorship We thank J.M. Mulet for his help with the quantification of intracellular ion concentrations, W.A. Prinz (NIH, Bethesda, MD) and A.K. Menon (Weill Cornell Medical College, New York) for the kind gift of the upc2-1 strain, F. Winston (Harvard Medical School, Boston) for the kind gift of the MOT3-18myc strain, and Avelino Corma (Instituto de Tecnologia Quimica, Valencia, Spain) for making available an ICP optical emission spectrometer for ion content determination. This work was supported by grants from Ministerio de Educacion y Ciencia (BFU2005-01714), from Ministerio de Ciencia e Innovacion (BFU2008-00271) and from Consejo Superior de Investigaciones Cientificas (200820I019). F.M. is recipient of an FPI predoctoral fellowship from Ministerio de Educacion y Ciencia. en_EN
dc.language Inglés es_ES
dc.publisher WILEY-BLACKWELL PUBLISHING, INC es_ES
dc.relation.ispartof Molecular Microbiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Activated protein-kinase es_ES
dc.subject Leucine zipper protein es_ES
dc.subject Saccharomyces-cerevisiae es_ES
dc.subject Osmotic-stress es_ES
dc.subject High-osmolarity es_ES
dc.subject Budding yeast es_ES
dc.subject Sterol homeostasis es_ES
dc.subject Ion homeostasis es_ES
dc.subject Gene-expression es_ES
dc.subject Salt stress es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Repression of ergosterol biosynthesis is essential for stress resistance and is mediated by the Hog1 MAP kinase and the Mot3 and Rox1 transcription factors es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/j.1365-2958.2010.07502.x es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MEC//BFU2005-01714/ES/ANALISIS GENÓMICO Y BIOQUÍMICO DE LOS BLANCOS DE LA MAP QUINASA HOG1 DURANTE LA ADAPTACION TRANSCRIPCIONAL A ESTRÉS OSMÓTICO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2008-00271/ES/RESPUESTA A ESTRES OSMOTICO EN SACCHAROMYCES Y ARABIDOPSIS: REGULACION DE LA CROMATINA Y DE LA ACTIVIDAD MITOCONDRIAL/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/CSIC//200820I019/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Martínez Montañés, FV.; Pascual-Ahuir Giner, MD.; Proft ., MH. (2010). Repression of ergosterol biosynthesis is essential for stress resistance and is mediated by the Hog1 MAP kinase and the Mot3 and Rox1 transcription factors. Molecular Microbiology. 79(4):1008-1023. https://doi.org/10.1111/j.1365-2958.2010.07502.x es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1111/j.1365-2958.2010.07502.x es_ES
dc.description.upvformatpinicio 1008 es_ES
dc.description.upvformatpfin 1023 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 79 es_ES
dc.description.issue 4 es_ES
dc.relation.pasarela S\39212 es_ES
dc.contributor.funder Ministerio de Educación y Ciencia es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Consejo Superior de Investigaciones Científicas


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