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Preparation of organic monolithic columns in polytetrafluoroethylene tubes for reversed-phase liquid chromatography

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Preparation of organic monolithic columns in polytetrafluoroethylene tubes for reversed-phase liquid chromatography

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dc.contributor.author Catalá-Icardo, Mónica es_ES
dc.contributor.author Torres-Cartas, Sagrario es_ES
dc.contributor.author Meseguer-Lloret, S. es_ES
dc.contributor.author Gómez Benito, Carmen es_ES
dc.contributor.author Carrasco-Correa, Enrique es_ES
dc.contributor.author Simó-Alfonso, Ernesto F. es_ES
dc.contributor.author Ramis-Ramos, Guillermo es_ES
dc.contributor.author Herrero-Martínez, José Manuel es_ES
dc.date.accessioned 2018-03-25T04:13:18Z
dc.date.available 2018-03-25T04:13:18Z
dc.date.issued 2017 es_ES
dc.identifier.issn 0003-2670 es_ES
dc.identifier.uri http://hdl.handle.net/10251/99709
dc.description.abstract [EN] In this work, a method for the preparation and anchoring of polymeric monoliths in a polytetrafluoroethylene (PTFE) tubing as a column housing for microbore HPLC is described. In order to assure a covalent attachment of the monolith to the inner wall of the PTFE tube, a two-step procedure was developed. Two surface etching reagents, a commercial sodium naphthalene solution (Fluoroetch®), or mixtures of H2O2 and H2SO4, were tried and compared. Then, the obtained hydroxyl groups on the PTFE surface were modified by methacryloylation. Attenuated total reflectance Fourier-transform infrared (ATR-FTIR) spectroscopy and scanning electron microscopy (SEM) confirmed the successful modification of the tubing wall and the stable anchorage of monolith to the wall, respectively. Special emphasis was also put on the reduction of the unwanted effects of shrinking of monolith during polymerization, by using an external proper mold and by selecting the adequate monomers in order to increase the flexibility of the polymer. Poly(glycidyl methacrylate-co-divinylbenzene) monoliths were in situ synthesized by thermal polymerization within the confines of surface-vinylized PTFE tubes. The modified PTFE tubing tightly held the monolith, and the monolithic column exhibited good pressure resistance up to 20 MPa. The column performance was also evaluated via the isocratic separation of a series of alkylbenzenes in the reversed-phase mode. The optimized monolithic columns gave plate heights ranged between 70 and 80 um. The resulting monoliths were also satisfactorily applied to the separation of proteins. es_ES
dc.description.sponsorship This work was supported by projects CTQ2014-52765-R (MINECO of Spain and Fondo Europeo de Desarrollo Regional, FEDER) and PROMETEO/2016/145 (Conselleria d'Educacio, Investigacio, Cultura i Esport, Generalitat Valenciana, Spain). The authors also thank Dr. P. Amoros del Toro from Institute of Materials Science (University of Valencia) and Dr. S. Armenta from Department of Analytical Chemistry for their help in surface area and FT-IR measurements, respectively. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation MINECO/CTQ2014-52765-R es_ES
dc.relation GV/PROMETEO/2016/145 es_ES
dc.relation.ispartof Analytica Chimica Acta es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Polymer monolith es_ES
dc.subject Polytetrafluoroethylene es_ES
dc.subject Surface modification es_ES
dc.subject Monolith attachment es_ES
dc.subject Microbore column es_ES
dc.subject Reversed-phase liquid chromatography es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Preparation of organic monolithic columns in polytetrafluoroethylene tubes for reversed-phase liquid chromatography es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.aca.2017.01.012 es_ES
dc.rights.accessRights Abierto es_ES
dc.date.embargoEndDate 2019-04-01 es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Catalá-Icardo, M.; Torres-Cartas, S.; Meseguer-Lloret, S.; Gómez Benito, C.; Carrasco-Correa, E.; Simó-Alfonso, EF.; Ramis-Ramos, G.... (2017). Preparation of organic monolithic columns in polytetrafluoroethylene tubes for reversed-phase liquid chromatography. Analytica Chimica Acta. 960:160-167. doi:10.1016/j.aca.2017.01.012 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.aca.2017.01.012 es_ES
dc.description.upvformatpinicio 160 es_ES
dc.description.upvformatpfin 167 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 960 es_ES
dc.relation.pasarela S\327074 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


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