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C7(P32) and C6(P34) PR proteins induced in tomato leaves by citrus exocortis viroid infection are chitinases

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C7(P32) and C6(P34) PR proteins induced in tomato leaves by citrus exocortis viroid infection are chitinases

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dc.contributor.author García-Breijo, Francisco-José es_ES
dc.contributor.author Garro Galiana, Rafael es_ES
dc.contributor.author Conejero Tomás, Vicente es_ES
dc.date.accessioned 2018-03-25T04:25:22Z
dc.date.available 2018-03-25T04:25:22Z
dc.date.issued 1990 es_ES
dc.identifier.issn 0885-5765 es_ES
dc.identifier.uri http://hdl.handle.net/10251/99716
dc.description.abstract [EN] Two chitinases induced in tomato leaves (Lycopersicon esculentum Mill cv. Rutgers) by citrus exocortis viroid (CEV) infection were purified. Their molecular masses determined by sodium dodecyl sulfate-gel electrophoresis were 32 kDa and 34 kDa and by filtration through Sephadex G-100 were 23 kDa and 25 kDa, respectively. These chitinases (P32 and P34) have been shown to be identical to the tomato pathogenesis-related proteins C7 and C6. They were purified in three stages: ammonium sulphate fractionation, chitin affinity chromatography and CM-Sepharose chromatography. The characterization of P32, the major component of the CEV-induced chitinase activity, revealed a basic protein (pI, 8·5), with a low optimum pH of 5·2 for hydrolytic activity, a high thermal stability and resistance to proteolytic degradation. These tomato chitinases have been shown to be serologically related to the tobacco chitinases. Our results lend further support to the hypothesis that enhanced chitinase activity is a component of a general mechanism of response to pathogens and other stress agents. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Physiological and Molecular Plant Pathology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject.classification BOTANICA es_ES
dc.subject.classification BIOLOGIA VEGETAL es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title C7(P32) and C6(P34) PR proteins induced in tomato leaves by citrus exocortis viroid infection are chitinases es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/0885-5765(90)90029-W es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ecosistemas Agroforestales - Departament d'Ecosistemes Agroforestals es_ES
dc.description.bibliographicCitation García-Breijo, F.; Garro Galiana, R.; Conejero Tomás, V. (1990). C7(P32) and C6(P34) PR proteins induced in tomato leaves by citrus exocortis viroid infection are chitinases. Physiological and Molecular Plant Pathology. 36(3):249-260. doi:10.1016/0885-5765(90)90029-W es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/0885-5765(90)90029-W es_ES
dc.description.upvformatpinicio 249 es_ES
dc.description.upvformatpfin 260 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 36 es_ES
dc.description.issue 3 es_ES
dc.relation.pasarela 23248 es_ES


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