Carbonell, Alberto; Flores Pedauye, Ricardo; Gago Zachert, Selma Persida(Oxford University Press (OUP): Policy C - Option B, 2011)
[EN] Trans-cleaving hammerheads with discontinuous or extended stem I and with tertiary stabilizing motifs (TSMs) have been tested previously against short RNA substrates in vitro at low Mg2+ concentration. However, the ...
[EN] Artificial microRNAs (amiRNAs) are highly specific, 21-nucleotide (nt) small RNAs designed to silence target transcripts. In plants, their application as biotechnological tools for functional genomics or crop improvement ...
Cuperus, Josh T.; CARBONELL, ALBERTO; Fahlgren, Noah; Garcia-Ruiz, Hernan; Burke, Russell T.; Takeda, Atsushi; Sullivan, Christopher M.; Gilbert, Sunny D.; Montgomery, Taiowa A.; Carrington, James C.(Nature Publishing Group, 2010-08)
[EN] NA interference pathways can involve amplification of secondary siRNAs by RNA-dependent RNA polymerases. In plants, RDR6-dependent secondary siRNAs arise from transcripts targeted by some microRNAs (miRNAs). Here, ...
[EN] Viroids, due to their small size and lack of protein-coding capacity, must rely essentially on their hosts for replication. Intriguingly, viroids have evolved the ability to replicate in two cellular organella, the ...
[EN] Viroids are small (246¿401 nucleotides), non-coding,
circular RNAs able to replicate autonomously in certain plants.
Viroids are classified into the families Pospiviroidae and
Avsunviroidae, whose members replicate ...
Maliogka, Varvara I.; Salvador, Beatriz; CARBONELL, ALBERTO; Saenz, Pilar; San Leon, David; Oliveros, Juan Carlos; Delgadillo, Ma Otilia; García, Juan Antonio; Simon-Mateo, Carmen(Blackwell Publishing, 2012-10)
[EN] Subisolates segregated from an M-type Plum pox virus (PPV) isolate, PPV-PS, differ widely in pathogenicity despite their high degree of sequence similarity. A single amino acid substitution, K109E, in the helper ...