Teixeira Miranda, William
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- PublicationAn all-in-one point-of-care testing device for multiplexed detection of respiratory infections(Elsevier, 2022-10-01) Teixeira Miranda, William; Pallás Tamarit, Yeray; Juste Dolz, Augusto Miguel; Sena-Torralba, Amadeo; Gozalbo-Rovira, Roberto; Rodríguez-Díaz, Jesús; Navarro, David; Carrascosa Rubio, Javier; Giménez-Romero, David; Maquieira Catala, Ángel; Morais Ezquerro, Sergi Beñat; Escuela Técnica Superior de Ingeniería de Telecomunicación; Departamento de Comunicaciones; Escuela Técnica Superior de Ingeniería Aeroespacial y Diseño Industrial; Departamento de Química; Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico; Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural; Grupo de Medioambiente y Seguridad Industrial. MEDASEGI; Centro de Investigación e Innovación en Bioingeniería; GENERALITAT VALENCIANA; AGENCIA ESTATAL DE INVESTIGACION; European Regional Development Fund; Universitat Politècnica de València[EN] The impact of the COVID-19 pandemic has reinforced the need for rapid, cost-effective, and reliable point-of-care testing (POCT) devices for massive population screening. The co-circulation of SARS-CoV-2 with several seasonal respiratory viruses highlights the need for multiplexed biosensing approaches. Herein, we present a fast and robust all-in-one POCT device for parallel viral antigen and serological analysis. The biosensing approach consists of a functionalized polycarbonate disc-shaped surface with microfluidic structures, where specific bioreagents are immobilized in microarray format, and a portable optoelectronic analyzer. The biosensor quantifies the concentration of viral antigens and specific immunoglobulins G and M for SARS-CoV-2, influenza A/B, adenovirus, and respiratory syncytial virus, using 30¿¿L of a sample. The semi-automated analysis of 6 samples is performed in 30¿min. Validation studies performed with 135 serum samples and 147 nasopharyngeal specimens reveal high diagnostic sensitivity (98¿100%) and specificity (84¿98%), achieving an excellent agreement (¿¿=¿0.937) with commercial immunoassays, which complies with the World Health Organization criteria for POC COVID-19 diagnostic tests. The versatility of the POCT device paves the way for the detection of other pathogens and analytes in the incoming post-pandemic world, integrating specific bioreagents against different variants of concerns and interests.
- PublicationWater-assisted synthesis of stable and multicolored CsPbX3@SiO2 core shell nanoparticles as fluorescent probes for biosensing(The Royal Society of Chemistry, 2023-11-15) Collantes-Pablo, Cynthia; Teixeira Miranda, William; González Pedro, María Victoria; Bañuls Polo, María José; Quintero Campos, Pedro; Morais Ezquerro, Sergi Beñat; Maquieira Catala, Ángel; Escuela Técnica Superior de Ingeniería Aeroespacial y Diseño Industrial; Departamento de Química; Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico; Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural; GENERALITAT VALENCIANA; Agencia Estatal de Investigación; UNIVERSIDAD POLITECNICA DE VALENCIA[EN] Colloidal lead halide perovskite nanocrystals are highly luminescent materials with great promise as fluorescent probes in biosensing as long as their intrinsic instability in aqueous media is effectively addressed. In this study, we successfully prepared stable and multicolored CsPbX3@SiO2 (X = Cl/Br, Br and I) core¿ shell nanoparticles through a simple method based on the water-induced transformation of Cs4PbX6 into CsPbX3, combined with sol¿gel procedures. We observed that the concentration of the Cs4PbX6 precursor plays a crucial role in the formation of isolated nanospheres with uniform silica coating and in controlling the number of core-free particles. Furthermore, our research expands this approach to other halide compositions, resulting in multicolored core¿shell nanoparticles with emission wavelengths ranging from 490 to 700 nm, average sizes below 30 nm, and photoluminescence quantum yields close to 60%. Unlike in previous reports, the silica coating boosts the photoluminescence quantum yields compared to uncoated counterparts and provides increased structural stability for more than four days. Moreover, a controlled thermal treatment confers water stability to the as-synthesized nanoparticles. To establish the feasibility of the developed materials as fluorescent probes, we successfully demonstrated their specific recognition of a humanized antibody (omalizumab) used in treating patients with severe allergic asthma. This work paves the way to develop in vitro tests using CsPbX3@SiO2 core¿shell nanoparticles as fluorogenic probes.
- PublicationDesigning stable lead halide perovskite nanocrystals: From a single particle to nanocomposites(Elsevier BV, 2023-04) Collantes-Pablo, Cynthia; Teixeira Miranda, William; González Pedro, María Victoria; Bañuls Polo, María José; Maquieira Catala, Ángel; Escuela Técnica Superior de Ingeniería Aeroespacial y Diseño Industrial; Departamento de Química; Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico; Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural; GENERALITAT VALENCIANA; AGENCIA ESTATAL DE INVESTIGACION; European Regional Development Fund; UNIVERSIDAD POLITECNICA DE VALENCIA[EN] Metal halide perovskite nanocrystals have attracted substantial interest given their easy manufacturing, superior Photoluminiscence Quantum Yield and striking optical properties. Despite the huge potential of such materials, their practical implementation and future technological applications need to overcome stability drawbacks: spontaneous degradation, which is accelerated by external stressors (i.e., moisture, oxygen, heat, light, and their combinations), poor phase stability and loss of their colloidal stability due to ligand lability. Within this framework, the understanding of their surface chemistry features and ligand-binding patterns plays a key role in improving the robustness and stability of perovskite nanocrystals. This review presents a comprehensive study of state-of-the-art and current challenges in surface chemistry, interface engineering and encapsulation methodologies for stabilizing lead halide perovskite nanoparticles. We first introduced lead halide perovskite structural and optical properties and a brief discussion of synthesis methods. Next, we explored recent developments in encapsulation methods in different protective matrices comprising from core-shell to macroscale nanocomposites. We also analyzed the advantages and shortcomings of each approach according to their final applications. Finally, we concluded with a discussion of open research challenges and future directions in the aforementioned aspects.