Godoy Reyes, Tania Mariel

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Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico

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https://www.upv.es/ficha-personal/tago

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https://panorama.upv.es/es/ipublic/researcher/341271

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Now showing 1 - 6 of 6

A l-glutamate-responsive delivery system based on enzyme-controlled self-immolative arylboronate-gated nanoparticles
(Royal Society of Chemistry, 2019-04-07) Godoy Reyes, Tania Mariel; Llopis Lorente, Antoni; García Fernández, Alba; Gaviña, Pablo; Costero, Ana M.; Villalonga, Reynaldo; Sancenón Galarza, Félix; Martínez Mañez, Ramón; Departamento de Química; Escuela Técnica Superior de Ingeniería de Edificación; Escuela Técnica Superior de Ingeniería Industrial; Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico; GENERALITAT VALENCIANA; MINISTERIO DE ECONOMIA Y COMPETITIVIDAD; Ministerio de Economía y Competitividad; Fundació Bancària Caixa d'Estalvis i Pensions de Barcelona
[EN] We report herein a l-glutamate (l-Glu)-responsive delivery system. It consists of Janus Au-mesoporous silica (MS) nanoparticles functionalized with l-glutamate oxidase on the Au face and with self-immolative arylboronate derivatives as caps on the MS face. The MS face is additionally loaded with a cargo. The delivery paradigm is based on the recognition of l-Glu by the enzyme and the subsequent formation of H2O2, which induces the cleavage of the self-immolative gate and the uncapping of the pores. Given the importance of l-Glu as a key neurotransmitter, we hope that these findings will help in designing new therapeutic strategies for nervous system diseases.
Desarrollo de un sensor químico basado en nanopartículas de oro para la detección colorimétrica de serotonina
(Universitat Politècnica de València, 2017-04-25) Godoy Reyes, Tania Mariel; Costero Nieto, Ana María; Martínez Mañez, Ramón; Departamento de Química; Escuela Técnica Superior de Ingeniería Industrial; Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico
El presente trabajo final de máster se basa en el diseño, síntesis y evaluación de un sensor colorimétrico, basado en nanopartículas de oro, capaz de reconocer la presencia de serotonina. La serotonina es un neurotransmisor responsable de diversas funciones en el cuerpo como puede ser la regulación del estado de ánimo, la digestión o el sueño entre otras. Además, a diferentes concentraciones se encuentra vinculado con el desarrollo de patologías psiquiátricas y tumores carcinoides. Se han estudiado los diferentes sistemas de detección de serotonina desarrollados hasta ahora que se citan en la introducción; además en ésta se describen de forma amplia las propiedades y aplicaciones de las nanopartículas de oro como sensores colorimétricos. El sensor colorimétrico diseñado consta de nanopartículas de oro cuya superficie se encuentra difuncionalizada por dos ligandos tiolados que reconocen selectivamente los grupos hidroxilo y amino de la serotonina, dichos ligandos son propionato de ditiobisuccinimidilo (DSP) y N-acetil-L-cisteína (NAC). Se encontró mediante empleo de UV-Vis que en presencia de serotonina, la banda de absorción de plasmón de las nanopartículas se desplaza hacia longitudes de onda más altas como consecuencia de la agregación de las mismas. Este hecho fue comprobado por imágenes de TEM y espectros de DLS y potencial Z. Los estudios de selectividad demostraron que el sensor es capaz de reconocer la serotonina en presencia de otros neurotransmisores. En cuanto a la sensibilidad, se determinó que el sensor colorimétrico es capaz de detectar el analito en el rango de concentraciones nanomolares de manera fiable.
Colorimetric detection of normetanephrine, a pheochromocytoma biomarker, using bifunctionalised gold nanoparticles
(Elsevier, 2019-05-16) Godoy Reyes, Tania Mariel; Costero, Ana M.; Gaviña, Pablo; Martínez Mañez, Ramón; Sancenón Galarza, Félix; Departamento de Química; Escuela Técnica Superior de Ingeniería Industrial; Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico; Generalitat Valenciana; Ministerio de Economía y Competitividad
[EN] A simple and effective colorimetric method for the detection of normetanephrine (NMN), an O-methylated metabolite of norepinephrine, using functionalised gold nanoparticles is described. This metabolite is an important biomarker in the diagnosis of adrenal tumours such as pheocromocytoma or paraganglioma. The colorimetric probe consists of spherical gold nanoparticles (AuNPs) functionalised with two different ligands, which specifically recognize different functional groups in normetanephrine. Thus, a benzaldehyde-terminated ligand was used for the recognition of the amino alcohol moiety in NMN, by forming the corresponding oxazolidine. On the other hand, N-acetyl-cysteine was chosen for the recognition of the phenolic hydroxyl group through the formation of hydrogen bonds. The selective double molecular recognition between the probe and the hydroxyl and the amino-alcohol moieties of normetanephrine led to interparticle-crosslinking aggregation resulting in a change in the color of the solution, from red to blue, which could be observed by naked eye. The probe was highly selective towards normetanephrine and no color changes were observed in the presence of other neurotransmitter metabolites such as homovanillic acid (HVA) (dopamine metabolite), 5-hydroxyindoleacetic acid (5-HIAA) (serotonin metabolite), or other biomolecules present in urine such as glucose (Glc), uric acid (U.A), and urea. Finally, the probe was evaluated in synthetic urine with constituents that mimic human urine, where a limit of detection of 0.5 mu M was achieved.
A Colorimetric Probe for the Selective Detection of Norepinephrine Based on a Double Molecular Recognition with Functionalized Gold Nanoparticles
(American Chemical Society, 2019-03-22) Godoy Reyes, Tania Mariel; Costero, Ana M.; Gaviña, Pablo; Martínez Mañez, Ramón; Sancenón Galarza, Félix; Departamento de Química; Escuela Técnica Superior de Ingeniería Industrial; Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico; Generalitat Valenciana; Ministerio de Economía y Competitividad
[EN] A simple colorimetric probe for the selective and sensitive detection of neurotransmitter norepinephrine (NE), an important biomarker in the detection of tumors such as pheochromocytoma and paraganglioma, is described. The sensing strategy is based on the use of spherical gold nanoparticles functionalized with benzaldehyde and boronic acid-terminated moieties. A double molecular recognition involving on one hand the aromatic aldehyde and the aminoalcohol group of NE, and on the other hand the boronic acid and the catechol moiety of the neurotransmitter, results in analyte triggered aggregation of the gold nanoparticles, leading to a bathochromic shift of the SPR band in the UV-vis spectrum of the probe and a clear change in the color of the solution from red to blue. Probe P1 shows a remarkable selectivity toward NE versus other catecholamine neurotransmitters (dopamine and epinephrine) and selected biomolecules (S-HIAA, L-Tyr, glucose, uric acid, Lys and glutamic acid). Moreover, a linear response to NE in the 0-1 mu M concentration range was observed and a limit of detection of 0.07 mu M in aqueous media was determined by UV-vis spectroscopy. The sensitivity of the probe toward NE in synthetic urine was also evaluated. In this medium, a limit of detection of 0.09 mu M was obtained which falls within the range of clinical interest
Selective and sensitive colorimetric detection of the neurotransmitter serotonin based on the aggregation of bifunctionalised gold nanoparticles
(Elsevier, 2018) Godoy Reyes, Tania Mariel; Llopis Lorente, Antoni; Costero, Ana M.; Sancenón Galarza, Félix; Gaviña, Pablo; Martínez Mañez, Ramón; Departamento de Química; Escuela Técnica Superior de Ingeniería de Edificación; Escuela Técnica Superior de Ingeniería Industrial; Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico; Generalitat Valenciana; Ministerio de Economía y Competitividad; Ministerio de Economía, Industria y Competitividad
[EN] We report a simple, sensitive and selective method for the colorimetric detection of serotonin (5-HT) in aqueous media using bifunctionalized gold nanoparticles (AuNPs). The probe (1) consisted of AuNPs functionalised with dithiobis(succinimidylpropionate) (DSP) and N-acetyl-l-cysteine (NALC). DSP was chosen to react with the amino group of 5-HT, whereas NALC was chosen to bind the hydroxyl group in 5-HT through hydrogen bonding and electrostatic interactions. A double interaction between nanoparticles and the hydroxyl and the amino group of serotonin led to interparticle-crosslinking aggregation. This, resulted in a colour change from red to blue that can be observed by the naked eye. The probe was selective to 5-HT and no colour modulation was observed in the presence of other neurotransmitters (i.e. dopamine, epinephrine, norepinephrine), selected biomolecules (i.e. L-tyrosine, gamma-aminobutyric acid, L-cysteine, uric acid, oxalic acid, aspartic acid and glutamic acid) and common inorganic species. A limit of detection as low as 0.1 mu M was determined in buffered water at pH 7 by UV-vis titrations. Similar response of the probe to 5-HT was observed in simulated blood serum, with a limit of detection of 0.12 mu M, and a linear response within the 0-3 mu M concentration range, which is within the range of the 5-HT concentrations of clinical interest. Finally, the performance of probe (1) in real human blood samples was evaluated, and showed a remarkable ability to distinguish between normal 5-HT levels and 5-HT levels indicative of disease. (c) 2017 Elsevier B.V. All rights reserved.
Acetylcholine-responsive cargo release using acetylcholinesterase-capped nanomaterials
(The Royal Society of Chemistry, 2019) Godoy Reyes, Tania Mariel; Llopis Lorente, Antoni; García Fernández, Alba; Gaviña, Pablo; Costero, Ana M.; Martínez Mañez, Ramón; Sancenón Galarza, Félix; Departamento de Química; Escuela Técnica Superior de Ingeniería de Edificación; Escuela Técnica Superior de Ingeniería Industrial; Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico; Generalitat Valenciana; Ministerio de Economía y Competitividad
[EN] Mesoporous silica nanoparticles capped with acetylcholinesterase, through boronic ester linkages, selectively release an entrapped cargo in the presence of acetylcholine.