Expression of xyloglucan endotransglucosylase/hydrolase (XTH) genes and XET activity in ethylene treated apple and tomato fruits

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dc.contributor.authorMuñoz Bertomeu, Jesúses_ES
dc.contributor.authorMiedes, Evaes_ES
dc.contributor.authorLorences, E. P.es_ES
dc.contributor.funderGeneralitat Valenciana
dc.date.accessioned2017-01-12T11:13:46Z
dc.date.available2017-01-12T11:13:46Z
dc.date.issued2013-09-01
dc.description.abstract[EN] Xyloglucan endotransglucosylase/hydrolase (XTHs: EC 2.4.1.207 and/or EC 3.2.1.151), a xyloglucan modifying enzyme, has been proposed to have a role during tomato and apple fruit ripening by loosening the cell wall. Since the ripening of climacteric fruits is controlled by endogenous ethylene biosynthesis, we wanted to study whether XET activity was ethylene-regulated, and if so, which specific genes encoding ripening-regulated XTH genes were indeed ethylene-regulated. XET specific activity in tomato and apple fruits was significantly increased by the ethylene treatment, as compared with the control fruits, suggesting an increase in the XTH gene expression induced by ethylene. The 25 SlXTH protein sequences of tomato and the 11 sequences MdXTH of apple were phylogenetically analyzed and grouped into three major clades. The SlXTHs genes with highest expression during ripening were SlXTHS and SlXTHS from Group III-B, and in apple MdXTH2, from Group II, and MdXTH10, and MdXTH11 from Group III-B. Ethylene was involved in the regulation of the expression of different SlXTH and MdXTH genes during ripening. In tomato fruit fifteen different SlXTH genes showed an increase in expression after ethylene treatment, and the SlXTHs that were ripening associated were also ethylene dependent, and belong to Group III-B (SlXTHS and SlXTHS). In apple fruit, three MdXTH showed an increase in expression after the ethylene treatment and the only MdXTH that was ripening associated and ethylene dependent was MdXTH10 from Group III-B. The results indicate that XTH may play an important role in fruit ripening and a possible relationship between XTHs from Group III-B and fruit ripening, and ethylene regulation is suggested. (C) 2013 Elsevier GmbH. All rights reserved.en_EN
dc.description.accrualMethodSes_ES
dc.description.bibliographicCitationMuñoz Bertomeu, J.; Miedes, E.; Lorences, EP. (2013). Expression of xyloglucan endotransglucosylase/hydrolase (XTH) genes and XET activity in ethylene treated apple and tomato fruits. Journal of Plant Physiology. 170(13):1194-1201. https://doi.org/10.1016/j.jplph.2013.03.015es_ES
dc.description.issue13es_ES
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dc.description.sponsorshipThis work was funded by GVA, PROMETEO/2009/075. We wish to thank Mr. D.A. Lindsay for correcting the English version of the manuscript.
dc.description.upvformatpfin1201es_ES
dc.description.upvformatpinicio1194es_ES
dc.description.volume170es_ES
dc.identifier.doi10.1016/j.jplph.2013.03.015
dc.identifier.issn0176-1617
dc.identifier.pmid23628624
dc.identifier.urihttps://riunet.upv.es/handle/10251/76679
dc.languageIngléses_ES
dc.publisherElsevieres_ES
dc.relation.ispartofJournal of Plant Physiologyes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/GVA//PROMETEO09%2F2009%2F075/ES/Mejora de plantas con interés agronómico y forestal- MEPIAF/es_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.jplph.2013.03.015es_ES
dc.relation.senia255035es_ES
dc.rightsReconocimiento - No comercial - Sin obra derivada (by-nc-nd)es_ES
dc.rights.accessRightsAbiertoes_ES
dc.subjectCell walles_ES
dc.subjectFruit ripeninges_ES
dc.subjectMalus domesticaes_ES
dc.subjectSolanum lycopersicumes_ES
dc.subjectTransglucosylation and xyloglucanes_ES
dc.subject.classificationBIOQUIMICA Y BIOLOGIA MOLECULARes_ES
dc.titleExpression of xyloglucan endotransglucosylase/hydrolase (XTH) genes and XET activity in ethylene treated apple and tomato fruitses_ES
dc.typeArtículoes_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES
dspace.entity.typePublication
upv.uuid009cccbe-6c4f-40e2-872d-2f8a462ceba8es_ES

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