A hybrid pathway for self-sustained luminescence

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dc.contributor.affiliationInstituto Universitario Mixto de Biología Molecular y Celular de Plantas
dc.contributor.authorPalkina, Kseniia A.es_ES
dc.contributor.authorKarataeva, Tatiana A.es_ES
dc.contributor.authorPerfilov, Maxim M.es_ES
dc.contributor.authorFakhranurova, Liliia I.es_ES
dc.contributor.authorMarkina, Nadezhda M.es_ES
dc.contributor.authorGonzalez Somermeyer, Louisaes_ES
dc.contributor.authorGarcia-Perez, Elenaes_ES
dc.contributor.authorVázquez-Vilar, Martaes_ES
dc.contributor.authorRodriguez-Rodriguez, Martaes_ES
dc.contributor.authorVictor
dc.contributor.authorShakhova, Ekaterina S.es_ES
dc.contributor.authorMitiouchkina, Tatianaes_ES
dc.contributor.authorBelozerova, Olga A.es_ES
dc.contributor.authorKovalchuk, Sergey I.es_ES
dc.contributor.authorAlekberova, Annaes_ES
dc.contributor.authorOrzáez Calatayud, Diego Vicente
dc.contributor.funderRussian Science Foundationes_ES
dc.contributor.funderUK Research and Innovationes_ES
dc.contributor.funderAgencia Estatal de Investigaciónes_ES
dc.contributor.funderEuropean Regional Development Fundes_ES
dc.contributor.funderRussian Foundation for Basic Researches_ES
dc.date.accessioned2024-09-09T18:10:07Z
dc.date.available2024-09-09T18:10:07Z
dc.date.issued2024-03-08es_ES
dc.description.abstract[EN] The fungal bioluminescence pathway can be reconstituted in other organisms allowing luminescence imaging without exogenously supplied substrate. The pathway starts from hispidin biosynthesis-a step catalyzed by a large fungal polyketide synthase that requires a posttranslational modification for activity. Here, we report identification of alternative compact hispidin synthases encoded by a phylogenetically diverse group of plants. A hybrid bioluminescence pathway that combines plant and fungal genes is more compact, not dependent on availability of machinery for posttranslational modifications, and confers autonomous bioluminescence in yeast, mammalian, and plant hosts. The compact size of plant hispidin synthases enables additional modes of delivery of autoluminescence, such as delivery with viral vectors.en_EN
dc.description.accrualMethodSes_ES
dc.description.bibliographicCitationPalkina, KA.; Karataeva, TA.; Perfilov, MM.; Fakhranurova, LI.; Markina, NM.; Gonzalez Somermeyer, L.; Garcia-Perez, E.... (2024). A hybrid pathway for self-sustained luminescence. Science Advances. 10(10). https://doi.org/10.1126/sciadv.adk1992es_ES
dc.description.issue10es_ES
dc.description.sponsorshipThis study was partially funded by light Bio and Planta. the Synthetic biology Group is funded by the MRC London Institute of Medical Sciences (UKRi Mc-A658- 5QeA0). Cloning and luminescent assays performed in BY-2 were partially supported by RSF, project number 22-14-00400, https://rscf.ru/project/22-14-00400/. Plant transformations were funded by RFBR and MOST, project number 21-54-52004. Plant imaging experiments were funded by RSF, project number 22-74-00124, https://rscf.ru/project/22-74-00124/. viral delivery experiments were funded by the grant PID2019-108203RB-I00 Plan nacional I+D from the Ministerio de ciencia e innovacion (Spain) through the Agencia estatal de investigacion (cofinanced by the European Regional Development Fund)es_ES
dc.description.volume10es_ES
dc.identifier.doi10.1126/sciadv.adk1992es_ES
dc.identifier.eissn2375-2548es_ES
dc.identifier.pmcidPMC10923510es_ES
dc.identifier.pmid38457503es_ES
dc.identifier.urihttps://riunet.upv.es/handle/10251/207831
dc.languageIngléses_ES
dc.publisherAmerican Association for the Advancement of Science (AAAS)es_ES
dc.relation.ispartofScience Advanceses_ES
dc.relation.pasarelaS\522504es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108203RB-I00/ES/EXPLOITING THE MODULAR ARCHITECTURE OF CRISPR%2FCAS TO DESIGN PROGRAMMABLE GENE CIRCUITS AND OTHER NEW BREEDING TOOLS IN PLANTS/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/RFBR//21-54-52004/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/RSF//22-14-00400/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/RSF//22-74-00124/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/UKRI//UKRI MC-A658-5QEA0/es_ES
dc.relation.publisherversionhttps://doi.org/10.1126/sciadv.adk1992es_ES
dc.rightsReconocimiento (by)es_ES
dc.rights.accessRightsAbiertoes_ES
dc.subjectCell-Suspension Cultureses_ES
dc.subjectIII Polyketide Synthaseses_ES
dc.subjectChalcone Synthasees_ES
dc.subjectExpression Systemes_ES
dc.subjectBiosynthesises_ES
dc.subjectProteines_ES
dc.titleA hybrid pathway for self-sustained luminescencees_ES
dc.typeArtículoes_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES
dspace.entity.typePublication
person.identifier716339
person.identifier289266
relation.isAuthorOfPublicationbead48e2-d26b-4c34-8464-60ba2573e2fc
relation.isAuthorOfPublication6a8224c3-9b53-4587-9fa7-154ac664910c
relation.isAuthorOfPublication.latestForDiscoverybead48e2-d26b-4c34-8464-60ba2573e2fc
relation.isOrgUnitOfPublicatione7a4640e-8a10-48bc-8661-bb4fb3481bd0
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upv.uuid09c52065-98bb-409b-ad44-2183ea44d2f2es_ES

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