PME10 Is a Pectin Methylesterase Driving PME Activity and Immunity Against Botrytis cinerea in Grapevine (Vitis vinifera L.)

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dc.contributor.authorLagrèze, Jorgees_ES
dc.contributor.authorSantiago Pajuelo, Antonioes_ES
dc.contributor.authorCoculo, Danielees_ES
dc.contributor.authorRojas, Bárbaraes_ES
dc.contributor.authorPizzio, Gaston A.es_ES
dc.contributor.authorZhang, Chenes_ES
dc.contributor.authorTian, Meng-Boes_ES
dc.contributor.authorMalnoy, Mickaeles_ES
dc.contributor.authorVannozzi, Alessandroes_ES
dc.contributor.authorDalla Costa, Lorenzaes_ES
dc.contributor.authorLionetti, Vincenzoes_ES
dc.contributor.authorMatus, José Tomáses_ES
dc.contributor.authorMalacarne, Giuliaes_ES
dc.contributor.funderGeneralitat Valencianaes_ES
dc.contributor.funderChina Scholarship Counciles_ES
dc.contributor.funderAgencia Estatal de Investigaciónes_ES
dc.contributor.funderEuropean Regional Development Fundes_ES
dc.contributor.funderMinistero dell'Istruzione, dell'Università e della Ricercaes_ES
dc.date.accessioned2026-06-04T06:10:33Z
dc.date.available2026-06-04T06:10:33Z
dc.date.issued2025-11es_ES
dc.description.abstract[EN] Botrytis cinerea (Bc) is a major pathogen of cultivated grapevine (Vitis vinifera L.), with cell wall (CW) remodelling playing a critical role in fungal colonisation. CW-modifying enzymes, particularly pectin methylesterases (PMEs), produced by both host and pathogen, influence CW integrity and the outcome of infection. To explore the role of CW composition and remodelling in grapevine's response to Bc, we inoculated three genotypes with varying susceptibility at full flowering. Biochemical analysis of flowers and ripe berry skins revealed that the tolerant genotype exhibited significantly higher PME activity postinfection compared with the susceptible ones. Unbiased transcriptome analysis of infected flower tissues showed a more intense transcriptional response in the susceptible genotype, suggesting an ultimately ineffective attempt to restrict fungus spread. Expression profiling of 62 PME genes in this data set and public Bc-infected berry transcriptomes identified PME10 as the most strongly induced gene upon infection. PME10 knockout mutants displayed reduced PME activity and heightened susceptibility, while overexpression lines showed enhanced PME activity and reduced disease symptoms. Gene co-expression network analysis highlighted WRKY03, a defence-related transcription factor, as a putative regulator of PME10. DAP-seq, DAP-qPCR and dual luciferase assays confirmed direct binding and activation of the PME10 promoter by WRKY03. Altogether, this study demonstrates that PME10 is a functional PME contributing to grapevine immunity against B. cinerea, establishing it as a key component of the grapevine defence machinery against fungal pathogens.es_ES
dc.description.accrualMethodSes_ES
dc.description.bibliographicCitationLagrèze, J.; Santiago Pajuelo, A.; Coculo, D.; Rojas, B.; Pizzio, GA.; Zhang, C.; Tian, M.... (2025). PME10 Is a Pectin Methylesterase Driving PME Activity and Immunity Against Botrytis cinerea in Grapevine (Vitis vinifera L.). Plant Biotechnology Journal. 23(11):4981-4997. https://doi.org/10.1111/pbi.70279es_ES
dc.description.issue11es_ES
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dc.description.sponsorshipJ.L. was co-funded by Fondazione Edmund Mach and CAVIRO wine cooperative in the framework of the PhD project New strategies for Botrytis bunch rot control for a sustainable viticulture . V.L. is funded by European Union, Project ECS 0000024 Rome Technopole: CUP B83C22002820006 and Project ECS 00000043 Consorzio iNEST: CUP B43C22000450006, by the Italian MUR, project REACH-XY : CUP B93C22001920001 and PRIN2022 2022F8BZMX. J.T.M. is funded by grant PID2021-128865NB-I00 from the Ministerio de Ciencia, Innovación y Universidades (MCIU, Spain), Agencia Estatal de Investigación (AEI, Spain) and Fondo Europeo de Desarrollo Regional (FEDER, European Union). A.S.P. is funded by the PROMETEO scholarship (PROMETEO/2021/056-01) granted by the Generalitat Valenciana (GVA). M.-B.T. is supported by the China Scholarship Council (CSC) no. 202406350122.es_ES
dc.description.upvformatpfin4997es_ES
dc.description.upvformatpinicio4981es_ES
dc.description.volume23es_ES
dc.identifier.doi10.1111/pbi.70279es_ES
dc.identifier.issn1467-7644es_ES
dc.identifier.pmcidPMC12576464es_ES
dc.identifier.pmid40729521es_ES
dc.identifier.urihttps://riunet.upv.es/handle/10251/235741
dc.languageIngléses_ES
dc.publisherBlackwell Publishinges_ES
dc.relation.ispartofPlant Biotechnology Journales_ES
dc.relation.pasarelaS\587127es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-128865NB-I00/ES/VALIDACION Y VISUALIZACION DE REDES REGULADORAS DEL METABOLISMO ESPECIALIZADO DE PLANTAS INTEGRANDO METODOS OMICOS/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/CSC//202406350122/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/GVA//PROMETEO%2F2021%2F056/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MIUR//CUP B83C22002820006/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MIUR//CUP B43C22000450006/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MIUR//CUP B93C22001920001/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MIUR//2022F8BZMX/es_ES
dc.relation.publisherversionhttps://doi.org/10.1111/pbi.70279es_ES
dc.rightsReconocimiento (by)es_ES
dc.rights.accessRightsAbiertoes_ES
dc.subjectCell wall integrityes_ES
dc.subjectCRISPR/Cas9es_ES
dc.subjectExpression atlases_ES
dc.subjectGene co-expression networkes_ES
dc.subjectGene overexpressiones_ES
dc.subjectPlant immunityes_ES
dc.subjectPMEes_ES
dc.subjectRNA-seqes_ES
dc.titlePME10 Is a Pectin Methylesterase Driving PME Activity and Immunity Against Botrytis cinerea in Grapevine (Vitis vinifera L.)es_ES
dc.typeArtículoes_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES
dspace.entity.typePublication
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