Freshwater-adapted polychaetes exhibit a complete enzymatic machinery for synthesizing long-chain polyunsaturated fatty acids

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dc.contributor.authorBainour, Khalidaes_ES
dc.contributor.authorZulkifli, Nabilahes_ES
dc.contributor.authorSam, Ka-Keies_ES
dc.contributor.authorNavarro, Juan C.es_ES
dc.contributor.authorCastro, Luis Filipe C.es_ES
dc.contributor.authorGlasby, Christopher J.es_ES
dc.contributor.authorShu-Chien, Alexander C.es_ES
dc.contributor.authorMonroig, Óscares_ES
dc.contributor.funderGeneralitat Valencianaes_ES
dc.contributor.funderUniversity of Science, Malaysiaes_ES
dc.contributor.funderAgencia Estatal de Investigaciónes_ES
dc.contributor.funderEuropean Regional Development Fundes_ES
dc.contributor.funderMinistry of Higher Education, Malaysiaes_ES
dc.date.accessioned2026-05-28T10:55:55Z
dc.date.available2026-05-28T10:55:55Z
dc.date.issued2025-09-03es_ES
dc.description.abstract[EN] The sustainability of aquaculture is challenged by limited fishmeal and fish oil supplies, key sources of long-chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic acid (EPA, 20:5 n-3), docosahexaenoic acid (DHA, 22:6 n-3) and arachidonic acid (ARA, 20:4 n-6), essential for fish health and product quality. Polychaetes represent a promising alternative. While marine polychaetes show complete LC-PUFA biosynthetic pathways involving elongases (Elovl), front-end desaturases (Fed), and methyl-end desaturases (¿ des), freshwater species remain poorly studied. We hypothesize that freshwater-adapted polychaetes exhibit enhanced LC-PUFA biosynthesis to compensate for limited dietary sources in freshwater environments. This study focuses on Namalycastis rhodochorde, a freshwater nereid polychaete found in Southeast Asia. We isolated and characterized elongase and desaturase genes from N. rhodochorde using a yeast-based heterologous expression system. Our results revealed three Elovl (Elovl2/5, Elovl4, Elovl1/7) that elongate PUFA substrates from C18 to C22, two Fed (Fed1 with ¿5 and Fed2 with dual ¿6/¿8 activities), and two ¿ des: a ¿12 desaturase enabling linoleic acid (18:2 n-6) synthesis, and an ¿3 desaturase converting n-6 into n-3 PUFA. These findings indicate that N. rhodochorde has the enzymatic capacity to synthesize LC-PUFA like ARA and EPA, supporting its potential for sustainable biomass production using low-nutrient substrates.es_ES
dc.description.accrualMethodSes_ES
dc.description.bibliographicCitationBainour, K.; Zulkifli, N.; Sam, K.; Navarro, JC.; Castro, LFC.; Glasby, CJ.; Shu-Chien, AC.... (2025). Freshwater-adapted polychaetes exhibit a complete enzymatic machinery for synthesizing long-chain polyunsaturated fatty acids. Open Biology. 15(9). https://doi.org/10.1098/rsob.250159es_ES
dc.description.issue9es_ES
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dc.description.referencesBainour K Zulkifli N Sam KK Navarro JC Castro LFC Glasby CJ et al. 2025 Supplementary material from: Freshwater-adapted polychaetes exhibit a complete enzymatic machinery for synthesising long-chain chain-PUFA. Figshare. (doi:10.6084/m9.figshare.c.7968756)es_ES
dc.description.sponsorshipThe POLYPUFA grant PID2022-136234OB-C21 was funded by MICIU/AEI/10.13039/501100011033 and by FEDER, UE. Additionally, this study is part of the ThinkInAzul programme funded by the European Union NextGenerationEU (PRTR-C17. I1) and the Generalitat Valenciana (THINKINAZUL/2021/026). K.B. is funded by a predoctoral contract, Santiago Grisolia (GRISOLIA/2021/120), from the Generalitat Valenciana. K.-K.S. acknowledges funding from a postdoctoral contract with Universiti Sains Malaysia (USM.9/25 Jld.XIV). We are also grateful to the Malaysian Ministry of Higher Education for research funding to A.C.S.-C. and graduate assistantship to N.Z. through the Fundamental Research Grant Scheme FRGS/1/2022/STG03/USM/01/2.es_ES
dc.description.volume15es_ES
dc.identifier.doi10.1098/rsob.250159es_ES
dc.identifier.eissn2046-2441es_ES
dc.identifier.pmcidPMC12405942es_ES
dc.identifier.pmid40898956es_ES
dc.identifier.urihttps://riunet.upv.es/handle/10251/235489
dc.languageIngléses_ES
dc.publisherThe Royal Society Publishinges_ES
dc.relation.ispartofOpen Biologyes_ES
dc.relation.pasarelaS\561109es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-136234OB-C21/ES/ESTUDIO DE LOS MECANISMOS DE BIOSINTESIS DE OMEGA-3 EN POLIQUETOS PARA SU USO COMO RECURSO EN ACUICULTURA/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/GVA//GRISOLIA%2F2021%2F120/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/GVA//THINKINAZUL%2F2021%2F026/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/USM//FRGS%2F1%2F2022%2FSTG03%2FUSM%2F01%2F2/es_ES
dc.relation.publisherversionhttps://doi.org/10.1098/rsob.250159es_ES
dc.rightsReconocimiento (by)es_ES
dc.rights.accessRightsAbiertoes_ES
dc.subjectElovles_ES
dc.subjectFatty acyl desaturaseses_ES
dc.subjectFreshwater polychaeteses_ES
dc.subjectLC-PUFA biosynthesises_ES
dc.subjectNamalycastis rhodochordees_ES
dc.titleFreshwater-adapted polychaetes exhibit a complete enzymatic machinery for synthesizing long-chain polyunsaturated fatty acidses_ES
dc.typeArtículoes_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES
dspace.entity.typePublication
upv.uuid26d200ea-bce6-4058-ae85-c31432f293efes_ES

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