Role of atrial tissue remodeling on rotor dynamics an in vitro study

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dc.contributor.affiliationDepartamento de Ingeniería Electrónica
dc.contributor.affiliationEscuela Técnica Superior de Ingeniería de Telecomunicación
dc.contributor.affiliationInstituto Universitario de Tecnologías de la Información y Comunicaciones
dc.contributor.authorCliment, A.M.es_ES
dc.contributor.authorGuillem Sánchez, María Salud
dc.contributor.authorFuentes, L.es_ES
dc.contributor.authorLee, P.es_ES
dc.contributor.authorBollensdorff, C.es_ES
dc.contributor.authorFernandez-Santos, M.E.es_ES
dc.contributor.authorSuarez-Sancho, S.es_ES
dc.contributor.authorSanz-Ruiz, R.es_ES
dc.contributor.authorSanchez, P.L.es_ES
dc.contributor.authorAtienza, F.es_ES
dc.contributor.authorFernandez-Aviles, F.es_ES
dc.date.accessioned2016-05-17T11:42:23Z
dc.date.available2016-05-17T11:42:23Z
dc.date.issued2015-12-01
dc.description.abstractThe objective of this article is to present an in vitro model of atrial cardiac tissue that could serve to study the mechanisms of remodeling related to atrial fibrillation (AF). We analyze the modification on gene expression and modifications on rotor dynamics following tissue remodeling. Atrial murine cells (HL-1 myocytes) were maintained in culture after the spontaneous initiation of AF and analyzed at two time points: 3.1 +/- 1.3 and 9.7 +/- 0.5 days after AF initiation. The degree of electrophysiological remodeling (i.e., relative gene expression of key ion channels) and structural inhomogeneity was compared between early and late cell culture times both in nonfibrillating and fibrillating cell cultures. In addition, the electrophysiological characteristics of in vitro fibrillation [e.g., density of phase singularities (PS/cm2), dominant frequency, and rotor meandering] analyzed by means of optical mapping were compared with the degree of electrophysiological remodeling. Fibrillating cell cultures showed a differential ion channel gene expression associated with atrial tissue remodeling (i.e., decreased SCN5A, CACN1C, KCND3, and GJA1 and increased KCNJ2) not present in nonfibrillating cell cultures. Also, fibrillatory complexity was increased in late- vs. early stage cultures (1.12 +/- 0.14 vs. 0.43 +/- 0.19 PS/cm(2), P < 0.01), which was associated with changes in the electrical reentrant patterns (i.e., decrease in rotor tip meandering and increase in wavefront curvature). HL-1 cells can reproduce AF features such as electrophysiological remodeling and an increased complexity of the electrophysiological behavior associated with the fibrillation time that resembles those occurring in patients with chronic AF.es_ES
dc.description.accrualMethodSes_ES
dc.description.bibliographicCitationCliment, A.; Guillem Sánchez, MS.; Fuentes, L.; Lee, P.; Bollensdorff, C.; Fernandez-Santos, M.; Suarez-Sancho, S.... (2015). Role of atrial tissue remodeling on rotor dynamics an in vitro study. AJP - Heart and Circulatory Physiology. 309(11):H1964-H1973. doi:10.1152/ajpheart.00055.2015es_ES
dc.description.issue11es_ES
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dc.description.sponsorshipThis work was supported in part by grants from the Spanish Ministry of Science and Innovation (PLE2009-0152), the Instituto de Salud Carlos III (Ministry of Economy and Competitiveness, Spain: PI13-01882, PI13-00903, and TEC2013-50391-EXP), and the Red de Investigacion Cardiovacular (RIC) from Instituto de Salud Carlos III (Ministry of Economy and Competitiveness, Spain).en_EN
dc.description.upvformatpfinH1973es_ES
dc.description.upvformatpinicioH1964es_ES
dc.description.volume309es_ES
dc.identifier.doi10.1152/ajpheart.00055.2015
dc.identifier.issn0363-6135
dc.identifier.urihttps://riunet.upv.es/handle/10251/64249
dc.languageIngléses_ES
dc.publisherAmerican Physiological Societyes_ES
dc.relation.ispartofAJP - Heart and Circulatory Physiologyes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN//PLE2009-0152/ES/INVESTIGACION TRASLACIONAL PARA EL DESARROLLO DE UN BANCO DE MATRICES DE ORGANOS Y DE ORGANOS Y TEJIDOS BIOARTIFICIALES AUTOLOGOS PARA TRASPLANTE/ /es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//TEC2013-50391-EXP/ES/DESARROLLO DE COMPUTADORES LOGICOS BIOLOGICOS BASADOS EN COMUNICACION IONICA ENTRE CELULAS CARDIACAS EXCITABLES Y NO EXCITABLES./es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//PI13/01882/ES/Estudio preclínico de la implantación de parches de tejido cardiaco bioartificial electromecánicamente entrenados en un modelo de infarto de miocardio porcino/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//PI13/00903/ES/EEstudio preclínico de la implantación de parches de tejido cardiaco bioartificial electromecánicamente entrenados en un modelo de infarto de miocardio porcino. Desarrollo de bioreactores con estimulación electromecánica/es_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1152/ajpheart.00055.2015es_ES
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dc.relation.senia305193es_ES
dc.rightsReserva de todos los derechoses_ES
dc.rights.accessRightsAbiertoes_ES
dc.subjectAtrial Fibrillationes_ES
dc.subjectOptical mappinges_ES
dc.subject.classificationTECNOLOGIA ELECTRONICAes_ES
dc.titleRole of atrial tissue remodeling on rotor dynamics an in vitro studyes_ES
dc.typeArtículoes_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES
dspace.entity.typePublication
person.identifier9657
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