Love Wave Immunosensor for the Detection of Carbaryl Pesticide

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dc.contributor.affiliationDepartamento de Ingeniería Electrónica
dc.contributor.affiliationEscuela Técnica Superior de Ingeniería de Telecomunicación
dc.contributor.affiliationCentro de Investigación e Innovación en Bioingeniería
dc.contributor.authorRocha-Gaso, María-Isabeles_ES
dc.contributor.authorGarcía Narbón, José Vicente
dc.contributor.authorGarcía, Pabloes_ES
dc.contributor.authorMarch Iborra, Mª Del Carmenes_ES
dc.contributor.authorJiménez Jiménez, Yolanda
dc.contributor.authorFrancis, Laurent-Alaines_ES
dc.contributor.authorMontoya Baides, Ángeles_ES
dc.contributor.authorArnau Vives, Antonio
dc.contributor.funderMinisterio de Educación y Cienciaes_ES
dc.contributor.funderAWSensors S.L.es_ES
dc.contributor.funderMinisterio de Economía y Competitividades_ES
dc.date.accessioned2015-07-06T11:12:24Z
dc.date.available2015-07-06T11:12:24Z
dc.date.issued2014-09-03
dc.description.abstractA Love Wave (LW) immunosensor was developed for the detection of carbaryl pesticide. The experimental setup consisted on: a compact electronic characterization circuit based on phase and amplitude detection at constant frequency; an automated flow injection system; a thermal control unit; a custom-made flow-through cell; and Quartz /SiO2 LW sensors with a 40 μm wavelength and 120 MHz center frequency. The carbaryl detection was based on a competitive immunoassay format using LIB-CNH45 monoclonal antibody (MAb). Bovine Serum Albumin-CNH (BSA-CNH) carbaryl hapten-conjugate was covalently immobilized, via mercaptohexadecanoic acid self-assembled monolayer (SAM), onto the gold sensing area of the LW sensors. This immobilization allowed the reusability of the sensor for at least 70 assays without significant signal losses. The LW immunosensor showed a limit of detection (LOD) of 0.09 μg/L, a sensitivity of 0.31 μg/L and a linear working range of 0.14–1.63 μg/L. In comparison to other carbaryl immunosensors, the LW immunosensor achieved a high sensitivity and a low LOD. These features turn the LW immunosensor into a promising tool for applications that demand a high resolution, such as for the detection of pesticides in drinking water at European regulatory levels.es_ES
dc.description.accrualMethodSes_ES
dc.description.bibliographicCitationRocha-Gaso, M.; García Narbón, JV.; García, P.; March Iborra, MDC.; Jiménez Jiménez, Y.; Francis, L.; Montoya Baides, Á.... (2014). Love Wave Immunosensor for the Detection of Carbaryl Pesticide. Sensors. 14(9):16434-16453. https://doi.org/10.3390/s140916434es_ES
dc.description.issue9es_ES
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dc.description.sponsorshipThe author also would like to acknowledge the Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund (ERDF) for their financing support through the grant of the INNPACTO 2012 project (DETECTA IPT-2012-0154-300000), J.V. Garcia's Fellowship, AP2007-03745, of FPU (Formacion de Profesorado Universitario) program, M. I. Rocha-Gaso's PhD CONACyT Fellowship and AWSensors Inc. for its collaboration.en_EN
dc.description.upvformatpfin16453es_ES
dc.description.upvformatpinicio16434es_ES
dc.description.volume14es_ES
dc.identifier.doi10.3390/s140916434
dc.identifier.issn1424-8220
dc.identifier.pmcidPMC4208181en_EN
dc.identifier.pmid25192313en_EN
dc.identifier.urihttps://riunet.upv.es/handle/10251/52737
dc.languageIngléses_ES
dc.publisherMDPIes_ES
dc.relation.ispartofSensorses_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//IPT-2012-0154-300000/ES/DEMOSTRADOR TECNOLÓGICO BASADO EN TRANSDUCTORES ACÚSTICOS PARA APLICACIONES DE PREVENCIÓN Y DIAGNÓSTICO EN SALUD (DETECTA)/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MEC//AP2007-03745/ES/AP2007-03745/es_ES
dc.relation.publisherversionhttp://dx.doi.org/10.3390/s140916434es_ES
dc.relation.references10.1016/j.talanta.2008.12.058es_ES
dc.relation.references10.1002/1521-3773(20001117)39:22<4004::AID-ANIE4004>3.0.CO;2-2es_ES
dc.relation.references10.3390/s90705740es_ES
dc.relation.references10.1016/j.aca.2007.09.056es_ES
dc.relation.references10.1007/s00216-008-1911-5es_ES
dc.relation.references10.1088/0957-0233/20/12/124004es_ES
dc.relation.references10.1063/1.3598340es_ES
dc.relation.references10.1021/jf9506904es_ES
dc.relation.references10.1021/jf035382hes_ES
dc.relation.references10.1021/ac051233hes_ES
dc.relation.references10.1016/j.bios.2005.10.013es_ES
dc.relation.references10.1021/jf950691wes_ES
dc.relation.references10.3390/s110504702es_ES
dc.relation.senia269153
dc.rightsReserva de todos los derechoses_ES
dc.rights.accessRightsAbiertoes_ES
dc.subjectBiosensores_ES
dc.subjectCarbaryl pesticide detectiones_ES
dc.subjectImmunosensores_ES
dc.subjectLove Wave sensores_ES
dc.subjectSurface Acoustic Wave (SAW)es_ES
dc.subject.classificationTECNOLOGIA ELECTRONICAes_ES
dc.titleLove Wave Immunosensor for the Detection of Carbaryl Pesticidees_ES
dc.typeArtículoes_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES
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