AGO1 CONTROLS INFLORESCENCE ARCHITECTURE POSSIBLY BY REGULATING TFL1 EXPRESSION

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dc.contributor.affiliationInstituto Universitario Mixto de Biología Molecular y Celular de Plantas
dc.contributor.authorFernández Nohales, Pedroes_ES
dc.contributor.authorDomenech Mir, Mª Josées_ES
dc.contributor.authorMartínez De Alba, Angel Emilioes_ES
dc.contributor.authorMicol, JLes_ES
dc.contributor.authorPonce, M.R.es_ES
dc.contributor.authorMADUEÑO, FRANCISCO
dc.contributor.funderMinisterio de Ciencia e Innovación
dc.contributor.funderMinisterio de Economía y Competitividad
dc.contributor.funderGeneralitat Valenciana
dc.contributor.funderConsejo Superior de Investigaciones Científicas
dc.contributor.funderMinisterio de Educación y Cultura
dc.date.accessioned2017-10-20T07:18:57Z
dc.date.available2017-10-20T07:18:57Z
dc.date.issued2014es_ES
dc.description.abstract[EN] The TERMINAL FLOWER 1 (TFL1) gene is pivotal in the control of inflorescence architecture in arabidopsis. Thus, tfl1 mutants flower early and have a very short inflorescence phase, while TFL1-overexpressing plants have extended vegetative and inflorescence phases, producing many coflorescences. TFL1 is expressed in the shoot meristems, never in the flowers. In the inflorescence apex, TFL1 keeps the floral genes LEAFY (LFY) and APETALA1 (AP1) restricted to the flower, while LFY and AP1 restrict TFL1 to the inflorescence meristem. In spite of the central role of TFL1 in inflorescence architecture, regulation of its expression is poorly understood. This study aims to expand the understanding of inflorescence development by identifying and studying novel TFL1 regulators. Mutagenesis of an Arabidopsis thaliana line carrying a TFL1::GUS (beta-glucuronidase) reporter construct was used to isolate a mutant with altered TFL1 expression. The mutated gene was identified by positional cloning. Expression of TFL1 and TFL1::GUS was analysed by real-time PCR and histochemical GUS detection. Double-mutant analysis was used to assess the contribution of TFL1 to the inflorescence mutant phenotype. A mutant with both an increased number of coflorescences and high and ectopic TFL1 expression was isolated. Cloning of the mutated gene showed that both phenotypes were caused by a mutation in the ARGONAUTE1 (AGO1) gene, which encodes a key component of the RNA silencing machinery. Analysis of another ago1 allele indicated that the proliferation of coflorescences and ectopic TFL1 expression phenotypes are not allele specific. The increased number of coflorescences is suppressed in ago1 tfl1 double mutants. The results identify AGO1 as a repressor of TFL1 expression. Moreover, they reveal a novel role for AGO1 in inflorescence development, controlling the production of coflorescences. AGO1 seems to play this role through regulating TFL1 expression.en_EN
dc.description.accrualMethodSes_ES
dc.description.bibliographicCitationFernández Nohales, P.; Domenech Mir, MJ.; Martínez De Alba, AE.; Micol, J.; Ponce, M.; Madueño Albi, F. (2014). AGO1 CONTROLS INFLORESCENCE ARCHITECTURE POSSIBLY BY REGULATING TFL1 EXPRESSION. Annals of Botany. 114(7):1471-1481. https://doi.org/10.1093/aob/mcu132es_ES
dc.description.issue7es_ES
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dc.description.sponsorshipWe thank Herve Vaucheret for the ago1-26 seeds, Antonio Serrano-Mislata for the pBTG6 construct, and Cristina Ferrandiz for critical reading of the manuscript. The collaboration of the IBMCP staff from the greenhouse, sequencing and microscopy facilities is also acknowledged. This work was supported by grants from the Spanish Ministerio de Ciencia e Innovacion (BIO2009-10876 and CSD2007-00057), the Spanish Ministerio de Economia y Competitividad (BFU2012-38929) and the Generalitat Valenciana (ACOMP2012-101). P.F.N. was supported by a fellowship from the I3P program of CSIC.en_EN
dc.description.upvformatpfin1481es_ES
dc.description.upvformatpinicio1471es_ES
dc.description.volume114es_ES
dc.identifier.doi10.1093/aob/mcu132es_ES
dc.identifier.issn0305-7364es_ES
dc.identifier.urihttps://riunet.upv.es/handle/10251/89668
dc.languageIngléses_ES
dc.relation.ispartofAnnals of Botanyes_ES
dc.relation.pasarelaS\284725es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN//BIO2009-10876/ES/Arquitectura De La Inflorescencia; Genes Que Controlan La Identidad De Los Meristemos Del Tallo/
dc.relation.projectIDinfo:eu-repo/grantAgreement/GVA//ACOM%2FP2012%2F101/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MEC//CSD2007-00057/ES/Función y potencial biotecnológico de los factores de transcripción de las plantas./
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//BFU2012-38929/ES/CONTROL DE LA FLORACION: LOS FACTORES DE TRANSCRIPCION VOZ COMO NUEVOS REGULADORES TRANSVERSALES DE LA RED GENETICA./
dc.relation.publisherversionhttp:dx.doi.org/10.1093/aob/mcu132es_ES
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dc.relation.references10.1101/gad.1201404es_ES
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dc.relation.references10.1016/j.devcel.2011.03.019es_ES
dc.relation.references10.1007/s11103-005-5992-7es_ES
dc.rightsReserva de todos los derechoses_ES
dc.rights.accessRightsAbiertoes_ES
dc.subjectFlower developmentes_ES
dc.subjectTERMINAL FLOWER 1es_ES
dc.subjectTFL1es_ES
dc.subjectARGONAUTE1es_ES
dc.subjectAGO1es_ES
dc.subjectplant architecturees_ES
dc.subjectinflorescence architecturees_ES
dc.subjectfloweringes_ES
dc.subjectArabidopsis thalianaes_ES
dc.subject.classificationBIOQUIMICA Y BIOLOGIA MOLECULARes_ES
dc.titleAGO1 CONTROLS INFLORESCENCE ARCHITECTURE POSSIBLY BY REGULATING TFL1 EXPRESSIONes_ES
dc.typeArtículoes_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES
dspace.entity.typePublication
person.identifier184580
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