Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy

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dc.contributor.affiliationInstituto Universitario Mixto de Biología Molecular y Celular de Plantas
dc.contributor.authorSarrion-Perdigones, Alejandroes_ES
dc.contributor.authorPalací, Jorgees_ES
dc.contributor.authorGRANELL RICHART, ANTONIO
dc.contributor.authorOrzáez Calatayud, Diego Vicente
dc.date.accessioned2016-09-05T08:22:53Z
dc.date.available2016-09-05T08:22:53Z
dc.date.issued2014
dc.description.abstractGoldenBraid (GB) is an iterative and standardized DNA assembling system specially designed for Multigene Engineering in Plant Synthetic Biology. GB is based on restriction–ligation reactions using type IIS restriction enzymes. GB comprises a collection of standard DNA pieces named “GB parts” and a set of destination plasmids (pDGBs) that incorporate the multipartite assembly of standardized DNA parts. GB reactions are extremely efficient: two transcriptional units (TUs) can be assembled from several basic GBparts in one T-DNA less than 24 h. Moreover, larger assemblies comprising 4–5 TUs are routinely built in less than 2 working weeks. Here we provide a detailed view of the GB methodology. As a practical example, a Bimolecular Fluorescence Complementation construct comprising four TUs in a 12 kb DNA fragment is presented.es_ES
dc.description.accrualMethodSes_ES
dc.description.bibliographicCitationSarrion-Perdigones, A.; Palací, J.; Granell Richart, A.; Orzáez Calatayud, DV. (2014). Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy. Methods in Molecular Biology. 1116:133-151. doi:10.1007/978-1-62703-764-8_10es_ES
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dc.description.upvformatpfin151es_ES
dc.description.upvformatpinicio133es_ES
dc.description.volume1116es_ES
dc.identifier.doi10.1007/978-1-62703-764-8_10
dc.identifier.issn1064-3745
dc.identifier.urihttps://riunet.upv.es/handle/10251/68688
dc.languageIngléses_ES
dc.publisherHumana Presses_ES
dc.relation.ispartofMethods in Molecular Biologyes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1007/978-1-62703-764-8_10es_ES
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dc.relation.senia256762es_ES
dc.rightsReserva de todos los derechoses_ES
dc.rights.accessRightsAbiertoes_ES
dc.subjectSynthetic biologyes_ES
dc.subjectDNA assemblyes_ES
dc.subjectType IIS enzymeses_ES
dc.subjectPlant biotechnologyes_ES
dc.subjectMultigene constructses_ES
dc.subjectMultigene engineeringes_ES
dc.subject.classificationMICROBIOLOGIAes_ES
dc.subject.classificationBIOQUIMICA Y BIOLOGIA MOLECULARes_ES
dc.titleDesign and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategyes_ES
dc.typeArtículoes_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES
dspace.entity.typePublication
person.identifier190518
person.identifier289266
person.identifier.orcid0000-0003-4266-9581
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upv.uuida52db561-076d-4a2e-b30f-e2c4f93eeb47es_ES

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