Highly specific gene silencing in a monocot species by artificial microRNAs derived from chimeric MIRNA precursors

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dc.contributor.affiliationInstituto Universitario Mixto de Biología Molecular y Celular de Plantas
dc.contributor.authorCARBONELL, ALBERTO
dc.contributor.authorFahlgren, Noahes_ES
dc.contributor.authorMitchell, Skyleres_ES
dc.contributor.authorCox, Kevin L., Jr.es_ES
dc.contributor.authorReilly, Kevin C.es_ES
dc.contributor.authorMockler, Todd C.es_ES
dc.contributor.authorCarrington, James C.es_ES
dc.contributor.funderU.S. Department of Energyes_ES
dc.contributor.funderNational Science Foundation, EEUUes_ES
dc.contributor.funderNational Institutes of Health, EEUUes_ES
dc.contributor.funderNational Institute of Food and Agriculture, EEUUes_ES
dc.date.accessioned2021-02-13T04:32:04Z
dc.date.available2021-02-13T04:32:04Z
dc.date.issued2015-06es_ES
dc.description.abstract[EN] Artificial microRNAs (amiRNAs) are used for selective gene silencing in plants. However, current methods to produce amiRNA constructs for silencing transcripts in monocot species are not suitable for simple, cost-effective and large-scale synthesis. Here, a series of expression vectors based on Oryza sativa MIR390 (OsMIR390) precursor was developed for high-throughput cloning and high expression of amiRNAs in monocots. Four different amiRNA sequences designed to target specifically endogenous genes and expressed from OsMIR390-based vectors were validated in transgenic Brachypodium distachyon plants. Surprisingly, amiRNAs accumulated to higher levels and were processed more accurately when expressed from chimeric OsMIR390-based precursors that include distal stem-loop sequences from Arabidopsis thaliana MIR390a (AtMIR390a). In all cases, transgenic plants displayed the predicted phenotypes induced by target gene repression, and accumulated high levels of amiRNAs and low levels of the corresponding target transcripts. Genome-wide transcriptome profiling combined with 5¿-RLM-RACE analysis in transgenic plants confirmed that amiRNAs were highly specific.en_EN
dc.description.accrualMethodSes_ES
dc.description.bibliographicCitationCarbonell, A.; Fahlgren, N.; Mitchell, S.; Cox, KLJ.; Reilly, KC.; Mockler, TC.; Carrington, JC. (2015). Highly specific gene silencing in a monocot species by artificial microRNAs derived from chimeric MIRNA precursors. The Plant Journal. 82(6):1061-1075. https://doi.org/10.1111/tpj.12835es_ES
dc.description.issue6es_ES
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dc.description.sponsorshipWe thank Goretti Nguyen, Robyn Stevens, Jacob Mreen, Fangfang Ma and Madison Schniers for invaluable technical assistance, and Zacchery R. Smith for his initial contribution to develop the pH7WG2B-OsMIR390-B/c vector. Noah Fahlgren was supported by a USDA AFRI NIFA Postdoctoral Fellowship (MOW-2012-01361). This work was supported by grants from the National Science Foundation (MCB-1231726, MCB-1330562) and National Institutes of Health (AI043288) to James C. Carrington, and from the Department of Energy (DOE DE-SC0006627) to Todd C. Mockler.es_ES
dc.description.upvformatpfin1075es_ES
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dc.description.volume82es_ES
dc.identifier.doi10.1111/tpj.12835es_ES
dc.identifier.issn0960-7412es_ES
dc.identifier.pmcidPMC4464980es_ES
dc.identifier.pmid25809382es_ES
dc.identifier.urihttps://riunet.upv.es/handle/10251/161209
dc.languageIngléses_ES
dc.publisherBlackwell Publishinges_ES
dc.relation.ispartofThe Plant Journales_ES
dc.relation.pasarelaS\378028es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/NSF//1231726/US/Function of Arabidopsis Small RNA-ARGONAUTE Complexes/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/NIH//AI043288/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/NSF//1330562/US/Integration of developmental signals by plant ARGONAUTES/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/NIFA//MOW-2012-01361/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/DOE//DE-SC0006627/es_ES
dc.relation.publisherversionhttps://doi.org/10.1111/tpj.12835es_ES
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dc.rightsReconocimiento (by)es_ES
dc.rights.accessRightsAbiertoes_ES
dc.subjectRNA silencinges_ES
dc.subjectArtificial microRNAes_ES
dc.subjectMIRNA precursores_ES
dc.subjectBrachypodium distachyones_ES
dc.subjectMonocotes_ES
dc.subjectArabidopsis thalianaes_ES
dc.subjectTechnical advancees_ES
dc.titleHighly specific gene silencing in a monocot species by artificial microRNAs derived from chimeric MIRNA precursorses_ES
dc.typeArtículoes_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES
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