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Covalent attachment of biotinylated molecular beacons via thiol-ene coupling. A study on conformational changes upon hybridization and streptavidin binding

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Covalent attachment of biotinylated molecular beacons via thiol-ene coupling. A study on conformational changes upon hybridization and streptavidin binding

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Gonzalez-Lucas, D.; Bañuls Polo, M.; García-Rupérez, J.; Maquieira Catala, A. (2017). Covalent attachment of biotinylated molecular beacons via thiol-ene coupling. A study on conformational changes upon hybridization and streptavidin binding. Microchimica Acta. 184(9):3231-3238. doi:10.1007/s00604-017-2310-4

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Title: Covalent attachment of biotinylated molecular beacons via thiol-ene coupling. A study on conformational changes upon hybridization and streptavidin binding
Author:
UPV Unit: Universitat Politècnica de València. Departamento de Comunicaciones - Departament de Comunicacions
Universitat Politècnica de València. Departamento de Química - Departament de Química
Issued date:
Abstract:
[EN] The authors describe a method for the detection of DNA by using immobilized molecular beacons (MBs) on the surface of silicon, with a view on possible application in biosensing. MB hybridization and protein recognition ...[+]
Subjects: Click chemistry , Dual polarization interferometry , Biosensing,Silicon on insulator , Hybridization
Copyrigths: Reserva de todos los derechos
Source:
Microchimica Acta. (issn: 0026-3672 )
DOI: 10.1007/s00604-017-2310-4
Publisher:
Springer-Verlag
Publisher version: http://doi.org/10.1007/s00604-017-2310-4
Project ID: info:eu-repo/grantAgreement/EC/H2020/644242/EU
Thanks:
This research was funded by the European Union Horizon 2020 program (ICT-644242 SAPHELY project), the MINECO projects CTQ/2013/45875-R, and CTQ/2016/75749-R, FEDER, and GVA PROMETEO II 2014/40.
Type: Artículo

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