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Covalent attachment of biotinylated molecular beacons via thiol-ene coupling. A study on conformational changes upon hybridization and streptavidin binding

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Covalent attachment of biotinylated molecular beacons via thiol-ene coupling. A study on conformational changes upon hybridization and streptavidin binding

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Gonzalez-Lucas, D.; Bañuls Polo, M.; García-Rupérez, J.; Maquieira Catala, Á. (2017). Covalent attachment of biotinylated molecular beacons via thiol-ene coupling. A study on conformational changes upon hybridization and streptavidin binding. Microchimica Acta. 184(9):3231-3238. https://doi.org/10.1007/s00604-017-2310-4

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Título: Covalent attachment of biotinylated molecular beacons via thiol-ene coupling. A study on conformational changes upon hybridization and streptavidin binding
Autor: Gonzalez-Lucas, Daniel Bañuls Polo, María-José García-Rupérez, Jaime Maquieira Catala, Ángel
Entidad UPV: Universitat Politècnica de València. Departamento de Comunicaciones - Departament de Comunicacions
Universitat Politècnica de València. Departamento de Química - Departament de Química
Fecha difusión:
Resumen:
[EN] The authors describe a method for the detection of DNA by using immobilized molecular beacons (MBs) on the surface of silicon, with a view on possible application in biosensing. MB hybridization and protein recognition ...[+]
Palabras clave: Click chemistry , Dual polarization interferometry , Biosensing,Silicon on insulator , Hybridization
Derechos de uso: Reserva de todos los derechos
Fuente:
Microchimica Acta. (issn: 0026-3672 )
DOI: 10.1007/s00604-017-2310-4
Editorial:
Springer-Verlag
Versión del editor: http://doi.org/10.1007/s00604-017-2310-4
Código del Proyecto:
info:eu-repo/grantAgreement/EC/H2020/644242/EU/Self-amplified photonic biosensing platform for microRNA-based early diagnosis of diseases/
info:eu-repo/grantAgreement/MINECO//CTQ2013-45875-R/ES/BIOSENSADO EN SOPORTES INTERACTIVOS CON PROPIEDADES INTERFEROMETRICAS PARA APLICACIONES CLINICAS. DEMOSTRACION EN FARMACOGENETICA Y ALERGIAS MEDICAMENTOSAS/
info:eu-repo/grantAgreement/MINECO//CTQ2016-75749-R/ES/BIOSENSORES HOLOGRAFICOS. PRUEBA DE CONCEPTO Y DEMOSTRACION EN APLICACIONES CLINICAS/
info:eu-repo/grantAgreement/GVA//PROMETEOII%2F2014%2F040/ES/Estudio de estrategias fisico-químicas para el desarrollo de biosensores interferométricos en soportes interactivos de aplicación en clínica/
Agradecimientos:
This research was funded by the European Union Horizon 2020 program (ICT-644242 SAPHELY project), the MINECO projects CTQ/2013/45875-R, and CTQ/2016/75749-R, FEDER, and GVA PROMETEO II 2014/40.
Tipo: Artículo

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