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Covalent attachment of biotinylated molecular beacons via thiol-ene coupling. A study on conformational changes upon hybridization and streptavidin binding

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Covalent attachment of biotinylated molecular beacons via thiol-ene coupling. A study on conformational changes upon hybridization and streptavidin binding

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dc.contributor.author Gonzalez-Lucas, Daniel es_ES
dc.contributor.author Bañuls Polo, María-José es_ES
dc.contributor.author García-Rupérez, Jaime es_ES
dc.contributor.author Maquieira Catala, Ángel es_ES
dc.date.accessioned 2018-04-30T04:14:01Z
dc.date.available 2018-04-30T04:14:01Z
dc.date.issued 2017 es_ES
dc.identifier.issn 0026-3672 es_ES
dc.identifier.uri http://hdl.handle.net/10251/101164
dc.description.abstract [EN] The authors describe a method for the detection of DNA by using immobilized molecular beacons (MBs) on the surface of silicon, with a view on possible application in biosensing. MB hybridization and protein recognition were interrogated on silicon-oninsulator (SOI) surfaces by using fluorescently tagged probes. In order to better understand the conformational changes that occur to MBs upon hybridization, the process was studied by using dual polarization interferometry (DPI). A model system was developed that matches thickness, mass, and density parameters. The results experimentally demonstrate that hybridization promotes the displacement of a protein away from the surface. This finding may be further exploited in techniques such as photonic sensors, thereby paving the way to the design of more sensitive biosensors based on the use of MBs. es_ES
dc.description.sponsorship This research was funded by the European Union Horizon 2020 program (ICT-644242 SAPHELY project), the MINECO projects CTQ/2013/45875-R, and CTQ/2016/75749-R, FEDER, and GVA PROMETEO II 2014/40. es_ES
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation info:eu-repo/grantAgreement/MINECO//CTQ2013-45875-R/ES/BIOSENSADO EN SOPORTES INTERACTIVOS CON PROPIEDADES INTERFEROMETRICAS PARA APLICACIONES CLINICAS. DEMOSTRACION EN FARMACOGENETICA Y ALERGIAS MEDICAMENTOSAS/ es_ES
dc.relation AEI/CTQ2016-75749-R es_ES
dc.relation CEE/644242 es_ES
dc.relation GV/PROMETEOII/2014/040 es_ES
dc.relation.ispartof Microchimica Acta es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Click chemistry es_ES
dc.subject Dual polarization interferometry es_ES
dc.subject Biosensing,Silicon on insulator es_ES
dc.subject Hybridization es_ES
dc.subject.classification TEORIA DE LA SEÑAL Y COMUNICACIONES es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Covalent attachment of biotinylated molecular beacons via thiol-ene coupling. A study on conformational changes upon hybridization and streptavidin binding es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s00604-017-2310-4 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/644242/EU/Self-amplified photonic biosensing platform for microRNA-based early diagnosis of diseases/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Comunicaciones - Departament de Comunicacions es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Gonzalez-Lucas, D.; Bañuls Polo, M.; García-Rupérez, J.; Maquieira Catala, Á. (2017). Covalent attachment of biotinylated molecular beacons via thiol-ene coupling. A study on conformational changes upon hybridization and streptavidin binding. Microchimica Acta. 184(9):3231-3238. https://doi.org/10.1007/s00604-017-2310-4 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1007/s00604-017-2310-4 es_ES
dc.description.upvformatpinicio 3231 es_ES
dc.description.upvformatpfin 3238 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 184 es_ES
dc.description.issue 9 es_ES
dc.relation.pasarela S\350263 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder Ministerio de Economía, Industria y Competitividad es_ES
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