Resumen:
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[EN] Poly(vinylidene fluoride) (PVDF) microparticles have been produced by electrospraying as a suitable substrate for tissue engineering applications. The influence of the polymer solution concentration and processing ...[+]
[EN] Poly(vinylidene fluoride) (PVDF) microparticles have been produced by electrospraying as a suitable substrate for tissue engineering applications. The influence of the polymer solution concentration and processing parameters, such as electric field, flow rate and inner needle diameter, on microparticle size and distribution has been studied. Polymer concentration is the most influential parameter on PVDF microparticle formation. Higher concentrations promote the formation of fibers while dilute or semi dilute concentrations favor the formation of PVDF microparticles with average diameters ranging between 0.81 +/- 0.34 and 5.55 +/- 2.34 mu m. Once the formation of microparticles is achieved, no significant differences were found with the variation of other electrospray processing parameters. The electroactive beta-phase content, between 63 and 74%, and the crystalline phase content, between 45 and 55%, are mainly independent of the processing parameters. Finally, MC-3T3-E1 cell adhesion on the PVDF microparticles is assessed, indicating their potential use for biomedical applications.
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Agradecimientos:
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This work is funded by FEDER funds through the "Programa Operacional Fatores de Competitividade - COMPETE" and by national funds arranged by FCT-Fundacao para a Ciencia e a Tecnologia, project references NANO/NMed-SD/0156/2007, ...[+]
This work is funded by FEDER funds through the "Programa Operacional Fatores de Competitividade - COMPETE" and by national funds arranged by FCT-Fundacao para a Ciencia e a Tecnologia, project references NANO/NMed-SD/0156/2007, PTDC/CTM-NAN/112574/2009, PEST-C/FIS/UI607/2011 and PEST-C/QUI/UIO686/2013. The authors also thank funding from Matepro - Optimizing Materials and Processes", ref. NORTE-07-0124-FEDER-000037", co-funded by the "Programa Operacional Regional do Norte" (ON.2 - O Novo Norte), under the "Quadro de Referencia Estrategico Nacional" (QREN), through the "Fundo Europeu de Desenvolvimento Regional" (FEDER). The authors also thank support from the COST Action MP1003, 2010 'European Scientific Network for Artificial Muscles', MP1206 "Electrospun Nano-fibres for bio inspired composite materials and innovative industrial applications" and MP1301 "New Generation Biomimetic and Customized Implants for Bone Engineering". DMC, RG, CR and VS thank the FCT for the SFRH/BD/82411/2011, SFRH/BD/90215/2012, SFRH/BPD/90870/2012 and SFRH/BPD/64958/2009 grants, respectively. The authors also thank R. Machado e M. Casal do 3CBMA, (Centre of Molecular and Environmental Biology), Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal for useful discussions and allowing the use of specific equipment. CIBER-BBN is an initiative funded by the VI National R&D&i Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund and Ministerio de Economia y Competitividad, MINECO, through the MAT2013-46467-C4-1-R project.
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