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An SNP-based saturated genetic map and QTL analysis of fruit-related traits in Zucchini using Genotyping-by-sequencing

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An SNP-based saturated genetic map and QTL analysis of fruit-related traits in Zucchini using Genotyping-by-sequencing

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dc.contributor.author Montero-Pau, Javier es_ES
dc.contributor.author Blanca Postigo, José Miguel es_ES
dc.contributor.author Esteras Gómez, Cristina es_ES
dc.contributor.author Martínez Pérez, Eva María es_ES
dc.contributor.author GOMEZ P es_ES
dc.contributor.author Monforte Gilabert, Antonio José es_ES
dc.contributor.author Cañizares Sales, Joaquín es_ES
dc.contributor.author Picó Sirvent, María Belén es_ES
dc.date.accessioned 2018-06-08T04:22:30Z
dc.date.available 2018-06-08T04:22:30Z
dc.date.issued 2017 es_ES
dc.identifier.issn 1471-2164 es_ES
dc.identifier.uri http://hdl.handle.net/10251/103607
dc.description.abstract [EN] Background: Cucurbita pepo is a cucurbit with growing economic importance worldwide. Zucchini morphotype is the most important within this highly variable species. Recently, transcriptome and Simple Sequence Repeat (SSR)- and Single Nucleotide Polymorphism (SNP)-based medium density maps have been reported, however further genomic tools are needed for efficient molecular breeding in the species. Our objective is to combine currently available complete transcriptomes and the Zucchini genome sequence with high throughput genotyping methods, mapping population development and extensive phenotyping to facilitate the advance of genomic research in this species. Results: We report the Genotyping-by-sequencing analysis of a RIL population developed from the inter subspecific cross Zucchini x Scallop (ssp. pepo x ssp. ovifera). Several thousands of SNP markers were identified and genotyped, followed by the construction of a high-density linkage map based on 7,718 SNPs (average of 386 markers/linkage group) covering 2,817.6 cM of the whole genome, which is a great improvement with respect to previous maps. A QTL analysis was performed using phenotypic data obtained from the RIL population from three environments. In total, 48 consistent QTLs for vine, flowering and fruit quality traits were detected on the basis of a multiple-environment analysis, distributed in 33 independent positions in 15 LGs, and each QTL explained 1.5 62.9% of the phenotypic variance. Eight major QTLs, which could explain greater than 20% of the phenotypic variation were detected and the underlying candidate genes identified. Conclusions: Here we report the first SNP saturated map in the species, anchored to the physical map. Additionally, several consistent QTLs related to early flowering, fruit shape and length, and rind and flesh color are reported as well as candidate genes for them. This information will enhance molecular breeding in C. pepo and will assist the gene cloning underlying the studied QTLs, helping to reveal the genetic basis of the studied processes in squash. es_ES
dc.description.sponsorship This work has been carried out in the framework of the INIA projects RTA2011-00044-C02-1/2 and E-RTA2013-00020-C04-03 of the Spanish Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA) cofunded with FEDER funds (EU). en_EN
dc.language Inglés es_ES
dc.publisher Springer (Biomed Central Ltd.) es_ES
dc.relation.ispartof BMC Genomics es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Cucurbita pepo es_ES
dc.subject RIL es_ES
dc.subject GBS es_ES
dc.subject Cartography es_ES
dc.subject Phenotyping es_ES
dc.subject Candidate genes es_ES
dc.subject.classification GENETICA es_ES
dc.title An SNP-based saturated genetic map and QTL analysis of fruit-related traits in Zucchini using Genotyping-by-sequencing es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1186/s12864-016-3439-y es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//RTA2011-00044-C02-02/ES/Desarrollo competitivo de nuevos cultivares de calabacín y ampliación de la plataforma genómica para la mejora de esta hortaliza/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//RTA2013-00020-C04-03/ES/Identificación de resistencias a ToLCNDV en Cucurbitáceas y análisis genético de las mismas/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.description.bibliographicCitation Montero-Pau, J.; Blanca Postigo, JM.; Esteras Gómez, C.; Martínez Pérez, EM.; GOMEZ P; Monforte Gilabert, AJ.; Cañizares Sales, J.... (2017). An SNP-based saturated genetic map and QTL analysis of fruit-related traits in Zucchini using Genotyping-by-sequencing. BMC Genomics. 18(94):1-21. https://doi.org/10.1186/s12864-016-3439-y es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1186/s12864-016-3439-y es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 21 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 18 es_ES
dc.description.issue 94 es_ES
dc.identifier.pmid 28100189 en_EN
dc.identifier.pmcid PMC5241963 en_EN
dc.relation.pasarela S\324140 es_ES
dc.contributor.funder Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria es_ES
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