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High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCM) immunosensor for pesticide detection in honey

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High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCM) immunosensor for pesticide detection in honey

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dc.contributor.author Cervera-Chiner, Lourdes es_ES
dc.contributor.author Juan Borrás, María del Sol es_ES
dc.contributor.author March, Carmen es_ES
dc.contributor.author Arnau Vives, Antonio es_ES
dc.contributor.author Escriche Roberto, Mª Isabel es_ES
dc.contributor.author Montoya, Ángel es_ES
dc.contributor.author Jiménez Jiménez, Yolanda es_ES
dc.date.accessioned 2018-06-18T04:21:54Z
dc.date.available 2018-06-18T04:21:54Z
dc.date.issued 2018 es_ES
dc.identifier.issn 0956-7135 es_ES
dc.identifier.uri http://hdl.handle.net/10251/104243
dc.description.abstract [EN] Quantification of chemical residues in honey is a market requirement to ensure consumer safety. The most common method used to analyze these compounds is the LC/MS/MS methodology, which requires highly qualified technicians and a tedious pre-treatment of the sample. The honey-packaging industry needs cheaper and faster alternatives for routine control. HFF-QCM (High Fundamental Frequency Quartz Crystal Microbalance) sensors are becoming a good option due to their high sensitivity, fast detection and low cost, while avoiding complex sample pre-treatment. The HFF-QCM technology is based on piezoelectric sensors with frequencies in the range from several tenths of MHz to hundreds of MHz. In this work a 100 MHz HFF-QCM sensor was used in a monoclonal antibody-based competitive immunoassay for specific bio-recognition of carbaryl pesticide as testing contaminant. The work intends to validate the use of HFF-QCM technology, in comparison with liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique, for the detection of contaminants in honey. For this purpose, the validation criteria required by SANCO 12571/2013 guidance document were considered. The precision and accuracy (recovery) of both methods were determined by comparison of 5 replicates at 4 different concentrations (from 0 to 100 ¿g/kg) using the same honey matrix. HFF-QCM technology showed good accuracy, with recovery percentages always between 110 and 120%. As regards to precision, HFF-QCM coefficients of variation (CV) were around 10% higher than those recommended by GC SANCO 12571/2013. HFF-QCM limits of detection (LOD) and quantification (LOQ) were in the same order of magnitude as those for LC-MS/MS, which allows the analysis of carbaryl residues in honey under the established maximum residue limits (MRL), without sample pre-treatment. These results show that biosensors based on HFF-QCM technology has become a serious alternative to the traditional analytical techniques for food quality and safety applications. es_ES
dc.description.sponsorship The authors are grateful for financial support from the Ministry of Economy and Competitiveness of Spain under the project AGL2013-48646-R. We also want to thank “Generalitat Valenciana” for the financial support to hire research personnel through the grant “Ayudas para la contratación de personal investigador en formación de carácter predoctoral” to carry out this research (DOC Num.7615/15.09.2015).
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation MINECO/AGL2013-48646-R es_ES
dc.relation.ispartof Food Control es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject High-fundamental-frequency QCM es_ES
dc.subject Piezoelectric immunosensors es_ES
dc.subject Carbaryl es_ES
dc.subject Pesticides es_ES
dc.subject Honey es_ES
dc.subject.classification TECNOLOGIA DE ALIMENTOS es_ES
dc.subject.classification TECNOLOGIA ELECTRONICA es_ES
dc.title High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCM) immunosensor for pesticide detection in honey es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.foodcont.2018.04.026 es_ES
dc.rights.accessRights Abierto es_ES
dc.date.embargoEndDate 2019-10-01 es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Interuniversitario de Investigación en Bioingeniería y Tecnología Orientada al Ser Humano - Institut Interuniversitari d'Investigació en Bioenginyeria i Tecnologia Orientada a l'Ésser Humà es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Tecnología de Alimentos - Departament de Tecnologia d'Aliments es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Electrónica - Departament d'Enginyeria Electrònica es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Ingeniería de Alimentos para el Desarrollo - Institut Universitari d'Enginyeria d'Aliments per al Desenvolupament es_ES
dc.description.bibliographicCitation Cervera-Chiner, L.; Juan Borras, MDS.; March, C.; Arnau Vives, A.; Escriche Roberto, MI.; Montoya, Á.; Jiménez Jiménez, Y. (2018). High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCM) immunosensor for pesticide detection in honey. Food Control. 92:1-6. doi:10.1016/j.foodcont.2018.04.026 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.foodcont.2018.04.026 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 6 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 92 es_ES
dc.relation.pasarela S\361670 es_ES
dc.contributor.funder Ministerio de Economía, Industria y Competitividad es_ES


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