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Inheritance analysis and identification of SNP markers associated with ZYMV resistance in Cucurbita pepo

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Inheritance analysis and identification of SNP markers associated with ZYMV resistance in Cucurbita pepo

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dc.contributor.author Capuozzo, C. es_ES
dc.contributor.author Formisano, G es_ES
dc.contributor.author Iovieno, P es_ES
dc.contributor.author Andolfo, G es_ES
dc.contributor.author Tomassoli, L es_ES
dc.contributor.author Barbella, M.M. es_ES
dc.contributor.author Picó Sirvent, María Belén es_ES
dc.contributor.author Paris, H.S. es_ES
dc.contributor.author Ercolano, M.R. es_ES
dc.date.accessioned 2018-06-18T04:25:09Z
dc.date.available 2018-06-18T04:25:09Z
dc.date.issued 2017 es_ES
dc.identifier.issn 1380-3743 es_ES
dc.identifier.uri http://hdl.handle.net/10251/104252
dc.description.abstract [EN] Cucurbit crops are economically important worldwide. One of the most serious threats to cucurbit production is Zucchini yellow mosaic virus (ZYMV). Several resistant accessions were identified in Cucurbita moschata and their resistance was introgressed into Cucurbita pepo. However, the mode of inheritance of ZYMV resistance in C. pepo presents a great challenge to attempts at introgressing resistance into elite germplasm. The main goal of this work was to analyze the inheritance of ZYMV resistance and to identify markers associated with genes conferring resistance. An Illumina GoldenGate assay allowed us to assess polymorphism among nine squash genotypes and to discover six polymorphic single-nucleotide polymorphisms (SNPs) between two near-isogenic lines, "True French" (susceptible to ZYMV) and Accession 381e (resistant to ZYMV). Two F-2 and three BC1 populations obtained from crossing the ZYMV-resistant Accession 381e with two susceptible ones, the zucchini True French and the cocozelle "San Pasquale," were assayed for ZYMV resistance. Molecular analysis revealed an approximately 90% association between SNP1 and resistance, which was confirmed using High Resolution Melt (HRM) and a CAPS marker. Co-segregation up to 72% in populations segregating for resistance was observed for two other SNP markers that could be potentially linked to genes involved in resistance expression. A functional prediction of proteins involved in the resistance response was performed on genome scaffolds containing the three SNPs of interest. Indeed, 16 full-length pathogen recognition genes (PRGs) were identified around the three SNP markers. In particular, we discovered that two nucleotide-binding site leucine-rich repeat (NBS-LRR) protein-encoding genes were located near the SNP1 marker. The investigation of ZYMV resistance in squash populations and the genomic analysis performed in this work could be useful for better directing the introgression of disease resistance into elite C. pepo germplasm. es_ES
dc.description.sponsorship This work was supported by the Ministry of University and Research (GenHORT project). es_ES
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation.ispartof Molecular Breeding es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Cucurbita pepo es_ES
dc.subject Pathogen recognition genes es_ES
dc.subject SNP markers es_ES
dc.subject Squash es_ES
dc.subject ZYMV resistance es_ES
dc.subject.classification GENETICA es_ES
dc.title Inheritance analysis and identification of SNP markers associated with ZYMV resistance in Cucurbita pepo es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s11032-017-0698-5 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//RTA2013-00020-C04-03/ES/Identificación de resistencias a ToLCNDV en Cucurbitáceas y análisis genético de las mismas/ es_ES
dc.rights.accessRights Abierto es_ES
dc.date.embargoEndDate 2018-08-01 es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Capuozzo, C.; Formisano, G.; Iovieno, P.; Andolfo, G.; Tomassoli, L.; Barbella, M.; Picó Sirvent, MB.... (2017). Inheritance analysis and identification of SNP markers associated with ZYMV resistance in Cucurbita pepo. Molecular Breeding. 37(8). https://doi.org/10.1007/s11032-017-0698-5 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1007/s11032-017-0698-5 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 37 es_ES
dc.description.issue 8 es_ES
dc.relation.pasarela S\346334 es_ES
dc.contributor.funder Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria es_ES
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