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dc.contributor.author | Vilchez Olivencia, Maria Carmen | es_ES |
dc.contributor.author | MORINI, MARINA | es_ES |
dc.contributor.author | Peñaranda, D.S. | es_ES |
dc.contributor.author | Gallego Albiach, Victor | es_ES |
dc.contributor.author | Asturiano Nemesio, Juan Francisco | es_ES |
dc.contributor.author | Pérez Igualada, Luz María | es_ES |
dc.date.accessioned | 2018-06-28T04:32:55Z | |
dc.date.available | 2018-06-28T04:32:55Z | |
dc.date.issued | 2017 | es_ES |
dc.identifier.issn | 1095-6433 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/104749 | |
dc.description.abstract | [EN] The role of potassium from the seminal plasma and/or the activation media was examined by selectively removing from this media, and by testing the use of channel inhibitors and a K-ionophore. Sperm motility was measured using a CASA system, intracellular K+ and pH were measured by flow cytometry, and sperm head area was measured by ASMA: Automated Sperm Morphometry Analyses. Sperm motility was notably inhibited by the removal of K+ from the seminal plasma and by treatment with the K+ ionophore valinomycin. This therefore indicates that a reduction of K+ levels in the quiescent stage inhibits further motility. The normal decrease in sperm head area induced by seawater activation was altered by the removal of K+ from the seminal plasma, and an increase in the pH; in the quiescent stage was also induced. Intracellular pH (pH;) was quantitatively measured for the first time in European eel spermatozoa, being 7.2 in the quiescent stage and 7.1 post-activation. Intracellular and external pH levels influenced sperm motility both in the quiescent stage and at activation. The alkalinization of the pH; (by NH4Cl) inhibited sperm motility activation, while acidification (by Na-acetate) did not have any effect. Our results indicate that a pH gradient between the sperm cell and the seminal plasma is necessary for sperm motility activation. The presence of the ion K+ in the seminal plasma (or in the extender medium) is necessary in order to maintain sperm volume, intracellular pH and sperm motility. | es_ES |
dc.description.sponsorship | Funded from the SPERMOT project (Spanish Ministry of Science and Innovation, MINECO AGL2010-16009). M.C. Vilchez has a predoctoral grant from UPV PAID Subprogramme 2 (2011-S2-02-6521), Marina Morini has a predoctoral grant from Generalitat Valenciana (Programa Grisolia, GRISOLIA/2012/006), Victor Gallego has a postdoctoral contract from UPV (PAID-10-14), and David S. Penaranda was supported by MICINN (PTA2011-4948-1) and UPV (PTA2011-4948-I). Grants to attend meetings were received from COST Office (AQUAGAMETE COST Action: FA1205). | |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | MINECO/PTA2011-4948-I | es_ES |
dc.relation.ispartof | Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Anguilla anguilla | es_ES |
dc.subject | Ion channels | es_ES |
dc.subject | Flow cytometry | es_ES |
dc.subject | CASA | es_ES |
dc.subject | ASMA | es_ES |
dc.subject | Ionophore | es_ES |
dc.subject | Inhibitor | es_ES |
dc.subject.classification | BIOLOGIA ANIMAL | es_ES |
dc.subject.classification | PRODUCCION ANIMAL | es_ES |
dc.title | Role of potassium and pH on the initiation of sperm motility in the European eel | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.cbpa.2016.09.024 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/FA1205/EU/AQUAGAMETE COST Action/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//AGL2010-16009/ES/MECANISMOS FISIOLOGICOS IMPLICADOS EN LA ESPERMIACION Y EN LA ADQUISICION DE MOTILIDAD ESPERMATICA EN LA ANGUILA EUROPEA (ANGUILLA ANGUILLA)/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/UPV//PAID-2011-S2-02-6521/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/UPV//PAID-10-14/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//GRISOLIA%2F2012%2F006/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/UPV//PAID/2011-S2-02-6521/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/GV//GRISOLIA/2012/006/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//PTA2011-4948-I/ | |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal | es_ES |
dc.description.bibliographicCitation | Vilchez Olivencia, MC.; Morini, M.; Peñaranda, D.; Gallego Albiach, V.; Asturiano Nemesio, JF.; Pérez Igualada, LM. (2017). Role of potassium and pH on the initiation of sperm motility in the European eel. Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology. 203:210-219. https://doi.org/10.1016/j.cbpa.2016.09.024 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.cbpa.2016.09.024 | es_ES |
dc.description.upvformatpinicio | 210 | es_ES |
dc.description.upvformatpfin | 219 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 203 | es_ES |
dc.identifier.pmid | 27693817 | |
dc.relation.pasarela | S\318718 | es_ES |
dc.contributor.funder | Universitat Politècnica de València | es_ES |
dc.contributor.funder | Ministerio de Economía, Industria y Competitividad | es_ES |
dc.contributor.funder | Generalitat Valenciana | |
dc.contributor.funder | Ministerio de Ciencia e Innovación | |
dc.contributor.funder | European Commission |