Vilchez Olivencia, MC.; Morini, M.; Peñaranda, D.; Gallego Albiach, V.; Asturiano Nemesio, JF.; Pérez Igualada, LM. (2017). Role of potassium and pH on the initiation of sperm motility in the European eel. Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology. 203:210-219. https://doi.org/10.1016/j.cbpa.2016.09.024
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/104749
Título:
|
Role of potassium and pH on the initiation of sperm motility in the European eel
|
Autor:
|
Vilchez Olivencia, Maria Carmen
MORINI, MARINA
Peñaranda, D.S.
Gallego Albiach, Victor
Asturiano Nemesio, Juan Francisco
Pérez Igualada, Luz María
|
Entidad UPV:
|
Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal
Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal
|
Fecha difusión:
|
|
Resumen:
|
[EN] The role of potassium from the seminal plasma and/or the activation media was examined by selectively removing from this media, and by testing the use of channel inhibitors and a K-ionophore. Sperm motility was measured ...[+]
[EN] The role of potassium from the seminal plasma and/or the activation media was examined by selectively removing from this media, and by testing the use of channel inhibitors and a K-ionophore. Sperm motility was measured using a CASA system, intracellular K+ and pH were measured by flow cytometry, and sperm head area was measured by ASMA: Automated Sperm Morphometry Analyses. Sperm motility was notably inhibited by the removal of K+ from the seminal plasma and by treatment with the K+ ionophore valinomycin. This therefore indicates that a reduction of K+ levels in the quiescent stage inhibits further motility. The normal decrease in sperm head area induced by seawater activation was altered by the removal of K+ from the seminal plasma, and an increase in the pH; in the quiescent stage was also induced. Intracellular pH (pH;) was quantitatively measured for the first time in European eel spermatozoa, being 7.2 in the quiescent stage and 7.1 post-activation. Intracellular and external pH levels influenced sperm motility both in the quiescent stage and at activation. The alkalinization of the pH; (by NH4Cl) inhibited sperm motility activation, while acidification (by Na-acetate) did not have any effect. Our results indicate that a pH gradient between the sperm cell and the seminal plasma is necessary for sperm motility activation. The presence of the ion K+ in the seminal plasma (or in the extender medium) is necessary in order to maintain sperm volume, intracellular pH and sperm motility.
[-]
|
Palabras clave:
|
Anguilla anguilla
,
Ion channels
,
Flow cytometry
,
CASA
,
ASMA
,
Ionophore
,
Inhibitor
|
Derechos de uso:
|
Reserva de todos los derechos
|
Fuente:
|
Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology. (issn:
1095-6433
)
|
DOI:
|
10.1016/j.cbpa.2016.09.024
|
Editorial:
|
Elsevier
|
Versión del editor:
|
https://doi.org/10.1016/j.cbpa.2016.09.024
|
Código del Proyecto:
|
info:eu-repo/grantAgreement/EC/H2020/FA1205/EU/AQUAGAMETE COST
Action/
MINECO/PTA2011-4948-I
...[+]
info:eu-repo/grantAgreement/EC/H2020/FA1205/EU/AQUAGAMETE COST
Action/
info:eu-repo/grantAgreement/MICINN//AGL2010-16009/ES/MECANISMOS FISIOLOGICOS IMPLICADOS EN LA ESPERMIACION Y EN LA ADQUISICION DE MOTILIDAD ESPERMATICA EN LA ANGUILA EUROPEA (ANGUILLA ANGUILLA)/
info:eu-repo/grantAgreement/UPV//PAID-2011-S2-02-6521/
info:eu-repo/grantAgreement/UPV//PAID-10-14/
info:eu-repo/grantAgreement/GVA//GRISOLIA%2F2012%2F006/
info:eu-repo/grantAgreement/UPV//PAID/2011-S2-02-6521/
info:eu-repo/grantAgreement/GV//GRISOLIA/2012/006/
info:eu-repo/grantAgreement/MICINN//PTA2011-4948-I/
MINECO/PTA2011-4948-I
[-]
|
Agradecimientos:
|
Funded from the SPERMOT project (Spanish Ministry of Science and Innovation, MINECO AGL2010-16009). M.C. Vilchez has a predoctoral grant from UPV PAID Subprogramme 2 (2011-S2-02-6521), Marina Morini has a predoctoral grant ...[+]
Funded from the SPERMOT project (Spanish Ministry of Science and Innovation, MINECO AGL2010-16009). M.C. Vilchez has a predoctoral grant from UPV PAID Subprogramme 2 (2011-S2-02-6521), Marina Morini has a predoctoral grant from Generalitat Valenciana (Programa Grisolia, GRISOLIA/2012/006), Victor Gallego has a postdoctoral contract from UPV (PAID-10-14), and David S. Penaranda was supported by MICINN (PTA2011-4948-1) and UPV (PTA2011-4948-I). Grants to attend meetings were received from COST Office (AQUAGAMETE COST Action: FA1205).
[-]
|
Tipo:
|
Artículo
|