Rojo-Bartolomé, I.; Martínez-Miguel, L.; Lafont, A.; Vilchez Olivencia, MC.; Asturiano Nemesio, JF.; Pérez Igualada, LM.; Cancio, I. (2017). Molecular markers of oocyte differentiation in European eel during hormonally induced oogenesis. Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology. 211:17-25. https://doi.org/10.1016/j.cbpa.2017.05.018
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/104780
[EN] Reproduction in captivity is a key study issue in Anguilla anguilla as a possible solution for its dwindling population. Understanding the mechanisms controlling the production of ribosomal building blocks during ...[+]
[EN] Reproduction in captivity is a key study issue in Anguilla anguilla as a possible solution for its dwindling population. Understanding the mechanisms controlling the production of ribosomal building blocks during artificially induced oocyte maturation could be particularly interesting. Transcription levels of ribosomal biogenesis associated genes could be used as markers to monitor oogenesis. Eels from the Albufera Lagoon were injected with carp pituitary extract for 15 weeks and ovaries in previtellogenic (PV) stage (non-injected), in early-, mid-, late-vitellogenesis (EV, MV, LV), as well as in migratory nucleus stage (MN) were analysed. 5S rRNA and related genes were highly transcribed in ovaries with PV oocytes. As oocytes developed, transcriptional levels of genes related to 5S rRNA production (gtf3a), accumulation (gtf3a, 42sp43) and nucleocytoplasmic transport (tp15, tp111) and the 5S/18S rRNA index decreased (PV > EV > MV > LV > MN). On the contrary, 18S rRNA was at its highest at MN stage while ubtf1 in charge of activating RNA-polymerase I and synthesising 18S rRNA behaved as 5S related genes. Individuals that did not respond (NR) to the treatment showed 5S/18S index values similar to PV females, while studied genes showed EV/LV-like transcription levels. Therefore, NR females fail to express the largest rRNAs, which could thus be taken as markers of successful vitellogenesis progression. In conclusion, we have proved that the transcriptional dynamics of ribosomal genes provides useful tools to characterize induced ovarian development in European eels. In the future, such markers should be studied as putative indicators of response to hormonal treatments and of the quality of obtained eel oocytes.[-]
info:eu-repo/grantAgreement/MINECO//AGL2012-33477/ES/IDENTIFICACION MOLECULAR DEL SEXO, DEL ESTADIO REPRODUCTOR Y DE LA FECUNDIDAD EN PECES DE INTERES COMERCIAL: MECANISMOS, APLICACIONES Y EXPLOTACION/ ...[+]
info:eu-repo/grantAgreement/MINECO//AGL2012-33477/ES/IDENTIFICACION MOLECULAR DEL SEXO, DEL ESTADIO REPRODUCTOR Y DE LA FECUNDIDAD EN PECES DE INTERES COMERCIAL: MECANISMOS, APLICACIONES Y EXPLOTACION/ info:eu-repo/grantAgreement/EC/FP7/245257/EU/Reproduction of European Eel: Towards a Self-sustained Aquaculture/ info:eu-repo/grantAgreement/MINECO//AGL2015-63936-R/ES/DESARROLLO OOCITARIO EN PECES; DIFERENCIACION Y MADURACION HACIA LA FECUNDACION VS LA ATRESIA/ info:eu-repo/grantAgreement/COST//FA1205/EU/Assessing and improving the quality of aquatic animal gametes to enhance aquatic resources - The need to harmonize and standardize evolving methodologies, and improve transfer from academia to industry/AQUAGAMETE/ info:eu-repo/grantAgreement/Eusko Jaurlaritza//S-PE12UN086/ES/Identificación molecular del sexo, del estadio reproductor y de la fecundidad en poblaciones de peces salvajes y de cultivo: mecanismos, aplicaciones y explotación/OVUM II/ info:eu-repo/grantAgreement/Eusko Jaurlaritza//IT810-13/ES/Grupo consolidado tipo A "Biología Celular en Toxicología Ambiental" (BCTA)/ info:eu-repo/grantAgreement/UPV%2FEHU//UFI 11%2F37/ES/Unidad de Formación e Investigación UFI “Protección de la Salud de los Ecosistemas”/ [-]
Agradecimientos:
This work was supported by EU 7th Framework Programme (PRO EEL, grant agreement no. 245257 & AQUAGAMETE COST Action FA1205), Spanish MINECO (AGL2012-33477 and AGL2015-63936-R), Basque Government (S-PE12UN086 & IT810-13) & ...[+]
This work was supported by EU 7th Framework Programme (PRO EEL, grant agreement no. 245257 & AQUAGAMETE COST Action FA1205), Spanish MINECO (AGL2012-33477 and AGL2015-63936-R), Basque Government (S-PE12UN086 & IT810-13) & UPV/EHU (UFI 11/37). Some fish were supplied by the Hunting and Continental Fishing Service of Generalitat Valenciana. I.R.B holds a PhD fellowship of the Basque Government.[-]