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Within-host Evolution of Segments Ratio for the Tripartite Genome of Alfalfa Mosaic Virus

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Within-host Evolution of Segments Ratio for the Tripartite Genome of Alfalfa Mosaic Virus

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dc.contributor.author Wu, Beilei es_ES
dc.contributor.author Zwart, Mark P. es_ES
dc.contributor.author Sanchez Navarro, Jesus Angel es_ES
dc.contributor.author Elena Fito, Santiago Fco es_ES
dc.date.accessioned 2018-07-26T07:05:09Z
dc.date.available 2018-07-26T07:05:09Z
dc.date.issued 2017 es_ES
dc.identifier.issn 2045-2322 es_ES
dc.identifier.uri http://hdl.handle.net/10251/106293
dc.description.abstract [EN] The existence of multipartite viruses is an intriguing mystery in evolutionary virology. Several hypotheses suggest benefits that should outweigh the costs of a reduced transmission efficiency and of segregation of coadapted genes associated with encapsidating each segment into a different particle. Advantages range from increasing genome size despite high mutation rates, faster replication, more efficient selection resulting from reassortment during mixed infections, better regulation of gene expression, or enhanced virion stability and cell-to-cell movement. However, support for these hypotheses is scarce. Here we report experiments testing whether an evolutionary stable equilibrium exists for the three genomic RNAs of Alfalfa mosaic virus (AMV). Starting infections with different segment combinations, we found that the relative abundance of each segment evolves towards a constant ratio. Population genetic analyses show that the segment ratio at this equilibrium is determined by frequency-dependent selection. Replication of RNAs 1 and 2 was coupled and collaborative, whereas the replication of RNA 3 interfered with the replication of the other two. We found that the equilibrium solution is slightly different for the total amounts of RNA produced and encapsidated, suggesting that competition exists between all RNAs during encapsidation. Finally, we found that the observed equilibrium appears to be host-species dependent. es_ES
dc.description.sponsorship We thank Francisca de la Iglesia, Paula Agudo and Lorena Corachan for their dedication and expert technical assistance. This project was funded in part by grants BFU2015-65037P from the Spanish Ministry of Economy and Competitiveness-FEDER, PROMETEOII/2014/021 from Generalitat Valenciana and EvoEvo (ICT610427) from the European Commission 7th Framework Program to S.F.E. The China Scholarship Council and the Chinese Academy of Agricultural Sciences provided funding to B.W. es_ES
dc.language Inglés es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation GV/PROMETEOII/2014/021 es_ES
dc.relation MINECO/BFU2015-65037-P es_ES
dc.relation.ispartof Scientific Reports es_ES
dc.rights Reconocimiento (by) es_ES
dc.title Within-host Evolution of Segments Ratio for the Tripartite Genome of Alfalfa Mosaic Virus es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1038/s41598-017-05335-8 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/610427/EU es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Wu, B.; Zwart, MP.; Sanchez Navarro, JA.; Elena Fito, SF. (2017). Within-host Evolution of Segments Ratio for the Tripartite Genome of Alfalfa Mosaic Virus. Scientific Reports. 7. doi:10.1038/s41598-017-05335-8 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1038/s41598-017-05335-8 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 7 es_ES
dc.identifier.pmid 28694514 en_EN
dc.identifier.pmcid PMC5504059 en_EN
dc.relation.pasarela 356545 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad (MINECO) es_ES
dc.contributor.funder Generalitat Valenciana (GV) es_ES
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