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Functional characterization of AGAMOUS-subfamily members from cotton during reproductive development and in response to plant hormones

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Functional characterization of AGAMOUS-subfamily members from cotton during reproductive development and in response to plant hormones

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dc.contributor.author Menezes, S. es_ES
dc.contributor.author Artico, S. es_ES
dc.contributor.author Lima, C. es_ES
dc.contributor.author Muniz, S. es_ES
dc.contributor.author Berbel Tornero, Ana es_ES
dc.contributor.author Brilhante, O. es_ES
dc.contributor.author Grossi, M.F. es_ES
dc.contributor.author Ferrandiz Maestre, Cristina es_ES
dc.contributor.author Madueño Albi, Francisco es_ES
dc.contributor.author Alves, M. es_ES
dc.date.accessioned 2018-12-05T21:04:33Z
dc.date.available 2018-12-05T21:04:33Z
dc.date.issued 2017 es_ES
dc.identifier.issn 2194-7953 es_ES
dc.identifier.uri http://hdl.handle.net/10251/113504
dc.description.abstract [EN] Reproductive development in cotton, including the fruit and fiber formation, is a complex process; it involves the coordinated action of gene expression regulators, and it is highly influenced by plant hormones. Several studies have reported the identification and expression of the transcription factor family MADS-box members in cotton ovules and fibers; however, their roles are still elusive during the reproductive development in cotton. In this study, we evaluated the expression profiles of five MADS-box genes (GhMADS3, GhMADS4, GhMADS5, GhMADS6 and GhMADS7) belonging to the AGAMOUS-subfamily in Gossypium hirsutum. Phylogenetic and protein sequence analyses were performed using diploid (G. arboreum, G. raimondii) and tetraploid (G. barbadense, G. hirsutum) cotton genomes, as well as the AG-subfamily members from Arabidopsis thaliana, Petunia hybrida and Antirrhinum majus. qPCR analysis showed that the AG-subfamily genes had high expression during flower and fruit development in G. hirsutum. In situ hybridization analysis also substantiates the involvement of AG-subfamily members on reproductive tissues of G. hirsutum, including ovule and ovary. The effect of plant hormones on AG-subfamily genes expression was verified in cotton fruits treated with gibberellin, auxin and brassinosteroid. All the genes were significantly regulated in response to auxin, whereas only GhMADS3, GhMADS4 and GhMADS7 genes were also regulated by brassinosteroid treatment. In addition, we have investigated the GhMADS3 and GhMADS4 overexpression effects in Arabidopsis plants. Interestingly, the transgenic plants from both cotton AG-like genes in Arabidopsis significantly altered the fruit size compared to the control plants. This alteration suggests that cotton AG-like genes might act regulating fruit formation. Our results demonstrate that members of the AG-subfamily in G. hirsutum present a conserved expression profile during flower development, but also demonstrate their expression during fruit development and in response to phytohormones. es_ES
dc.description.sponsorship We thank Durvalina Felix and Alexandre Garcez by assist in samples preparation. We are grateful Fabia Guimaraes-Dias by valuable suggestions on the Manuscript. This work was supported by the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Fundacao de Amparo a Pesquisa do Rio de Janeiro (FAPERJ), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) and European Community (Evolutionary Conservation of Regulatory Network Controlling Flower Development, EVOCODE). es_ES
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation UE/FP7-PEOPLE-2009-IRSES es_ES
dc.relation.ispartof Plant Reproduction es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Gossypium hirsutum es_ES
dc.subject MADS-box genes es_ES
dc.subject Plant hormones es_ES
dc.subject Gene expression es_ES
dc.subject Reference genes es_ES
dc.subject Reproductive development es_ES
dc.title Functional characterization of AGAMOUS-subfamily members from cotton during reproductive development and in response to plant hormones es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s00497-017-0297-y es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/247587/EU es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Menezes, S.; Artico, S.; Lima, C.; Muniz, S.; Berbel Tornero, A.; Brilhante, O.; Grossi, M.... (2017). Functional characterization of AGAMOUS-subfamily members from cotton during reproductive development and in response to plant hormones. Plant Reproduction. 30(1):19-39. doi:10.1007/s00497-017-0297-y es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1007/s00497-017-0297-y es_ES
dc.description.upvformatpinicio 19 es_ES
dc.description.upvformatpfin 39 es_ES
dc.type.version info:eu repo/semantics/publishedVersion es_ES
dc.description.volume 30 es_ES
dc.description.issue 1 es_ES
dc.relation.pasarela 356329 es_ES
dc.contributor.funder UE es_ES


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