- -

Influence of inoculum type (ileal, caecal and faecal) on the in vitro fermentation of different sources of carbohydrates in rabbits

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

Influence of inoculum type (ileal, caecal and faecal) on the in vitro fermentation of different sources of carbohydrates in rabbits

Show simple item record

Files in this item

dc.contributor.author Abad-Guamán, Rodrigo es_ES
dc.contributor.author Larrea-Dávalos, Jose Antonio es_ES
dc.contributor.author Carabaño, Rosa es_ES
dc.contributor.author García, Javier es_ES
dc.contributor.author Carro, Maria Dolores es_ES
dc.date.accessioned 2019-01-03T12:32:17Z
dc.date.available 2019-01-03T12:32:17Z
dc.date.issued 2018-09-28
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/114685
dc.description.abstract [EN] Two in vitro experiments were performed to analyse the fermentative potential of ileal content, caecal content, soft faeces and hard faeces from adult rabbits. Experiment 1 evaluated 3 doses (0.5, 1.0 and 2.0 g fresh digesta/g substrate dry matter [DM]) of ileal and caecal digesta as inoculum in 28 h-incubations. Two ileal and 2 caecal inocula were obtained, each by pooling the ileal or caecal digesta of 2 adult rabbits. Pectin from sugar beet pulp (SBP) and the insoluble residue obtained after a 2-step in vitro pre-digestion of SBP and wheat straw were used as substrates. The 0.5 dose produced the lowest (P<0.05) amount of gas at 28 h, with no differences (P>0.05) between the 1.0 and 2.0 doses (44.9, 51.6 and 53.8 mL/g substrate DM, respectively; values averaged across inocula and substrates). Experiment 2 evaluated two doses of ileal inoculum (1 and 1.5 g fresh digesta/g substrate DM) and compared ileal digesta, caecal digesta, soft faeces and hard faeces as inoculum for determining in vitro gas production (144-h incubations) of the 3 substrates used in Experiment 1 and wheat starch. Three inocula of each type were obtained, each by pooling either digesta or faeces from 3 rabbits. There were no differences (P>0.05) between the 2 ileal doses tested in gas production parameters, and therefore the 1.0 dose was selected for further ileal fermentations. Starch and pectin showed similar (P>0.05) values of gas production rate and maximal gas production rate when they were fermented with caecal digesta (0.038 vs. 0.043%/h, and 13.7 vs. 15.2 mL/h, respectively), soft (0.022 vs. 0.031%/h, and 9.97 vs. 9.33 mL/h) and hard faeces (0.031 vs. 0.038%/h, and 13.6 vs. 10.8 mL/h), and values were higher than those for SBP and wheat straw; in contrast, values for starch and pectin differed with the ileal inoculum (0.046 vs. 0.024%/h, and 18.4 vs. 6.60 mL/h). Both ileal and caecal gas production parameters were well correlated with those for hard and soft faeces inocula, respectively (r≥0.77; P≤0.040). The ileal inoculum showed a relevant fermentative potential, but lower than that of caecal digesta and soft and hard faeces for all substrates except wheat starch. es_ES
dc.description.sponsorship Funding from the Spanish Ministry of Economy and Competitiveness (Project AGL2011-22628) and the Comunidad Autónoma de Madrid (CAM; Project MEDGAN ABI-2913) is gratefully acknowledged. es_ES
dc.language Inglés es_ES
dc.publisher Universitat Politècnica de València
dc.relation MINECO/AGL2011-22628 es_ES
dc.relation CAM/MEDGAN ABI-2913 es_ES
dc.relation.ispartof World Rabbit Science
dc.rights Reserva de todos los derechos es_ES
dc.subject In vitro es_ES
dc.subject Gas production es_ES
dc.subject Sugar beet pulp es_ES
dc.subject Pectin es_ES
dc.subject Wheat straw es_ES
dc.subject Starch es_ES
dc.subject Rabbit es_ES
dc.title Influence of inoculum type (ileal, caecal and faecal) on the in vitro fermentation of different sources of carbohydrates in rabbits es_ES
dc.type Artículo es_ES
dc.date.updated 2019-01-03T11:41:54Z
dc.identifier.doi 10.4995/wrs.2018.9726
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Abad-Guamán, R.; Larrea-Dávalos, JA.; Carabaño, R.; García, J.; Carro, MD. (2018). Influence of inoculum type (ileal, caecal and faecal) on the in vitro fermentation of different sources of carbohydrates in rabbits. World Rabbit Science. 26(3):227-240. doi:10.4995/wrs.2018.9726 es_ES
dc.relation.publisherversion https://doi.org/10.4995/wrs.2018.9726 es_ES
dc.description.upvformatpinicio 227 es_ES
dc.description.upvformatpfin 240 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 26
dc.description.issue 3
dc.identifier.eissn 1989-8886
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Comunidad de Madrid es_ES
dc.relation.references Abad R., Ibañez M.A., Carabaño R., García J. 2013. Quantification of soluble fibre in feedstuffs for rabbits and evaluation of the interference between the determinations of soluble fibre and intestinal mucin. Anim. Feed Sci. Tech., 182: 61-70. https://doi.org/10.1016/j.anifeedsci.2013.04.001 es_ES
dc.relation.references Abad-Guamán R., Carabaño R., Gómez-Conde M.S., García J. 2015. Effect of type of fiber, site of fermentation, and method of analysis on digestibility of soluble and insoluble fiber in rabbits. J. Anim. Sci., 93: 2860-2871. https://doi.org/10.2527/jas.2014-8767 es_ES
dc.relation.references Association of Official Analytical Chemists International. 2000. Official Methods of Analysis 17th ed. AOAC International, Washington, DC. es_ES
dc.relation.references Bindelle J., Buldgen A., Lambotte D., Wavreille J., Leterme P. 2007. Effect of pig faecal donor and of pig diet composition on in vitro fermentation of sugar beet pulp. Anim. Feed Sci. Technol., 132: 212-226. https://doi.org/10.1016/j.anifeedsci.2006.03.010 es_ES
dc.relation.references Boletín Oficial del Estado (BOE). 2013. Royal Decree 53/2013 of February 1st on the protection of animals used for experimentation or other scientific purposes. BOE nº 34, 11370-11421. https://www.boe.es/boe/dias/2013/02/08/pdfs/BOE-A-2013-1337.pdf Accessed January 2017. In Spanish. es_ES
dc.relation.references Bovera F., Calabro S., Cutrignelli M.I., Infascelli F., Piccolo G., Nizza S., Tudisco R., Nizza A. 2008. Prediction of rabbit caecal fermentation characteristics from faeces by in vitro gas production technique: roughages. J. Anim. Physiol. Anim. Nutr., 92: 260-271. https://doi.org/10.1111/j.1439-0396.2007.00748.x es_ES
dc.relation.references Bovera F., D'Urso S., Di Meo C., Piccolo G., Calabro S., Nizza A. 2006. Comparison of rabbit caecal content and rabbit hard faeces as source of inoculum for the in vitro gas production technique. Asian Austral. J. Anim. Sci., 19: 1649-1657. https://doi.org/10.5713/ajas.2006.1649 es_ES
dc.relation.references Bovera F., D'Urso S., Meo C.D., Tudisco R., Nizza A. 2009. A model to assess the use of caecal and faecal inocula to study fermentability of nutrients in rabbit. J. Anim. Physiol. Anim. Nutr., 93: 147-156. https://doi.org/10.1111/j.1439-0396.2007.00795.x es_ES
dc.relation.references Calabrò S., Nizza A., Pinna W., Cutrignelli M., Piccolo V. 1999. Estimation of digestibility of compound diets for rabbits using the in vitro gas production technique. World Rabbit Sci., 7: 197-201. https://doi.org/10.4995/wrs.1999.401 es_ES
dc.relation.references Carabaño R., Fraga M.J., Santoma G., de Blas C. 1988. Effect of diet on composition of cecal contents and on excretion and composition of soft and hard feces of rabbits. J. Anim. Sci 66: 901-1000. https://doi.org/10.2527/jas1988.664901x es_ES
dc.relation.references Carabaño R., García J., de Blas J.C. 2001. Effect of fibre source on ileal apparent digestibility of non-starch polysaccharides in rabbits. Anim. Sci., 72: 343-350. https://doi.org/10.1017/S1357729800055843 es_ES
dc.relation.references Falcão-e-Cunha L., Peres H., Freire J.P.B., Castro-Solla L. 2004. Effects of alfalfa, wheat bran or beet pulp, with or without sunflower oil, on caecal fermentation and on digestibility in the rabbit. Anim. Feed Sci. Technol., 117: 131-149. https://doi.org/10.1016/j.anifeedsci.2004.07.014 es_ES
dc.relation.references García J., Carabaño R., de Blas J.C. 1999. Effect of fiber source on cell wall digestibility and rate of passage in rabbits. J. Anim. Sci., 77: 898-905. https://doi.org/10.2527/1999.774898x es_ES
dc.relation.references García J., Carabaño R., Pérez-Alba L., de Blas J.C. 2000. Effect of fiber source on cecal fermentation and nitrogen recycled through cecotrophy in rabbits. J. Anim. Sci., 78: 638-646. https://doi.org/10.2527/2000.783638x es_ES
dc.relation.references García J., Gidenne T., Falcão-e-Cunha L., de Blas C. 2002. Identification of the main factors that influence caecal fermentation traits in growing rabbits. Anim. Res. 51: 165-173. https://doi.org/10.1051/animres:2002011 es_ES
dc.relation.references Gidenne T. 1992. Effect of fiber level, particle-size and adaptation period on digestibility and rate of passage as measured at the ileum and in the feces in the adult-rabbit. Brit. J. Nutr., 67: 133-146. https://doi.org/10.1079/BJN19920015 es_ES
dc.relation.references Gidenne T. 1994. Effect of a reduction in fiber content on the rate of passage through the digestive-tract of the rabbit-comparison of models for the fecal kinetics of 2 markers. Reprod. Nutr. Dev., 34: 295-307. https://doi.org/10.1051/rnd:19940403 es_ES
dc.relation.references Goering H.K., Van Soest P.J. 1970. Forage Fiber Analysis (Apparatus, Reagents, Procedures, and Some Applications). USDA Agricultural Research Service, Handbook, Washington, DC. es_ES
dc.relation.references Gouet P., Fonty G. 1979. Changes in the digestive microflora of holoxenic rabbits from birth until adulthood. Ann. Biol Anim. Bioch., 19: 553-566. https://doi.org/10.1051/rnd:19790501 es_ES
dc.relation.references Littell R.C., Henry P.R., Ammerman C.B. 1998. Statistical analysis of repeated measures data using SAS procedures. J. Anim. Sci., 76: 1216-1231. https://doi.org/10.2527/1998.7641216x es_ES
dc.relation.references Marounek M., Vovk S.J., Skrivanova V. 1995. Distribution of activity of hydrolytic enzymes in the digestive-tract of rabbits. Brit. J. Nutr., 73: 463-469. https://doi.org/10.1079/BJN19950048 es_ES
dc.relation.references Menke K.H., Raab L., Salewski A., Steingass H., Fritz D., Schneider W. 1979. The estimation of the digestibility and metabolizable energy of ruminant feedingstuff from the gas production when they are incubated with rumen liquor in vitro. J. Agr. Sci., 93: 217-222. https://doi.org/10.1017/S0021859600086305 es_ES
dc.relation.references Mertens D.R., Allen M., Carmany J., Clegg J., Davidowicz A., Drouches M., Frank K., Gambin D., Garkie M., Gildemeister B., Jeffress D., Jeon C.S., Jones D., Kaplan D., Kim G.N., Kobata S., Main D., Moua X., Paul B., Robertson J., Taysom D., Thiex N., Williams J., Wolf M. 2002. Gravimetric determination of amylase-treated neutral detergent fiber in feeds with refluxing in beakers or crucibles: Collaborative study. J. AOAC Int., 85:1217-1240. es_ES
dc.relation.references Mould F.L., Kliem K.E., Morgan R., Mauricio R.M. 2005. In vitro microbial inoculum: A review of its function and properties. Anim. Feed Sci. Tech., 123: 31-50. https://doi.org/10.1016/j.anifeedsci.2005.04.028 es_ES
dc.relation.references Murray S.M., Flickinger E.A., Patil A.R., Merchen N.R., Brent J.L., Fahey G.C. 2001. In vitro fermentation characteristics of native and processed cereal grains and potato starch using ileal chyme from dogs. J. Anim. Sci., 79: 435-444. https://doi.org/10.2527/2001.792435x es_ES
dc.relation.references Omed H.M., Lovett D.K., Axford R.F.E. 2000. Faeces as a source of microbial enzymes for estimating digestibility, In: Givens D., Owen E., Axford R., Omed H. (Eds.), Forage Evaluation in Ruminant Nutrition, CAB International, UK, pp. 135-154. https://doi.org/10.1079/9780851993447.0135 es_ES
dc.relation.references Padilha M.T.S., Licois D., Gidenne T., Carre B., Fonty G. 1995. Relationships between microflora and caecal fermentation in rabbits before and after weaning. Reprod. Nutr. Dev., 35: 375-386. https://doi.org/10.1051/rnd:19950403 es_ES
dc.relation.references Pascual J.J., Cervera C., Fernández-Carmona J. 2000. Comparison of different in vitro digestibility methods for nutritive evaluation of rabbit diets. World Rabbit Sci., 8: 93-97. https://doi.org/10.4995/wrs.2000.425 es_ES
dc.relation.references Penney R.L., Folk G.E., Galask R.P., Petzold C.R. 1986. The microflora of the alimentary tract of rabbits in relation to pH, diet and cold. J. Appl. Rabbit Res., 9: 152-156. es_ES
dc.relation.references Piattoni F., Demeyer D., Maertens L., 1997. Fasting effects on in vitro fermentation pattern of rabbit caecal contents. World Rabbit Sci., 5: 23-26. https://doi.org/10.4995/wrs.1997.314 es_ES
dc.relation.references Rodríguez-Romero N., Abecia L., Fondevila M., Balcells J. 2011. Effects of levels of insoluble and soluble fibre in diets for growing rabbits on faecal digestibility, nitrogen recycling and in vitro fermentation. World Rabbit Sci., 19: 85-94. https://doi.org/10.4995/wrs.2011.828 es_ES
dc.relation.references SAS Institute Inc. 2011. Base SAS® 9.3 Procedures Guide. SAS Institute Inc. Cary, NC, USA. es_ES
dc.relation.references Schofield P., Pitt R.E., Pell A.N. 1994. Kinetics of fiber digestion from in-vitro gas-production. J. Anim. Sci., 72: 2980-2991. https://doi.org/10.2527/1994.72112980x es_ES
dc.relation.references Tagliapietra F., Williams B.A., Awati A., Bonsembiante M., Schiavon S., Verstegen M.W.A. 2003. In vitro degradation kinetics of four carbohydrates using ileal and faecal inocula from suckling piglets. Ital. J. Anim. Sci., 2: 195-197. es_ES
dc.relation.references Trocino A., García J., Carabaño R., Xiccato, G. 2013. A meta-analysis on the role of soluble fibre in diets for growing rabbits. World Rabbit Sci., 21: 1-15. https://doi.org/10.4995/wrs.2013.1285 es_ES
dc.relation.references Van Soest P.J., Robertson J.B., Lewis B.A. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci., 74: 3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2 es_ES
dc.relation.references Wang D., Williams B.A., Ferruzzi M.G., D'Arcy B.R. 2013. Different concentrations of grape seed extract affect in vitro starch fermentation by porcine small and large intestinal inocula. J. Sci. Food Agr., 93: 276-283. https://doi.org/10.1002/jsfa.5753 es_ES
dc.relation.references Williams B.A., Bhatia S.K., Boer H., Tamminga S. 1995. A preliminary study using the cumulative gas production technique to compare the kinetics of different fermentations by use of standard substrates. Ann. Zootech., 44: 35. https://doi.org/10.1051/animres:19950505 es_ES
dc.relation.references Williams B.A., Bosch M.W., Awati A., Konstantinov S.R., Smidt H., Akkermans A.D.L., Verstegen M.W.A., Tamminga S. 2005. In vitro assessment of gastrointestinal tract (GIT) fermentation in pigs: Fermentable substrates and microbial activity. Anim. Res., 54: 191-201. https://doi.org/10.1051/animres:2005011 es_ES
dc.relation.references Williams B.A., Verstegen M.W., Tamminga S. 2001. Fermentation in the large intestine of single-stomached animals and its relationship to animal health. Nutr. Res. Rev., 14: 207-228. https://doi.org/10.1079/NRR200127 es_ES


This item appears in the following Collection(s)

Show simple item record