Lipolysis kinetics of milk-fat catalyzed by an enzymatic supplement under simulated gastrointestinal conditions

Abstract

[EN] Pancreatic insufficiency is a clinical manifestation characterized by the in-ability of the pancreas to release enough pancreatic enzyme into the small intestine, necessary to digest intraluminal nutrients. The lack of digestive enzymes leads to the difficulty to absorb nutrients, which drives in infants, to malnutrition and lack of growth and development, due to the loss of calories. These patients generally need oral administration of enzymes to favor lipolysis and absorption of lipids from foods. However, there are a number of food related factors (matrix, type of fat, etc.) and digestive environment (intestinal pH, bile concentration, among others), which will influence the digestibility of nutrients.

In this study, an "in vitro" digestion model was used to characterize the kinetics of the lipolysis of milk-fat catalyzed by an enzymatic supplement. Different intestinal conditions (pH (6, 7 and 8) and bile concentrations (1, 5 and 10 mml L-1)) were simulated, using a fixed concentration of supplement. Gastro-Intestinal conditions, significantly affected lipolysis. High pH and bile concentrations were translated into low values of the Michaelis-Menten constant and high values of the catalytic constant. The kinetic parameters obtained from this work allowed estimating the dose of enzymatic supplement required to optimize the lipolysis of milk-fat under different intestinal environments, sufficient and insufficient pancreatic conditions.