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PYL8 mediates ABA perception in the root through non-cell-autonomous and ligand-stabilization-based mechanisms

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PYL8 mediates ABA perception in the root through non-cell-autonomous and ligand-stabilization-based mechanisms

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dc.contributor.author Belda-Palazón, Borja es_ES
dc.contributor.author Gonzalez-Garcia, Mary-Paz es_ES
dc.contributor.author LOZANO JUSTE, JORGE es_ES
dc.contributor.author Coego Gonzalez, Alberto es_ES
dc.contributor.author Antoni-Alandes, Regina es_ES
dc.contributor.author Julian-Valenzuela, Jose es_ES
dc.contributor.author Peirats-Llobet, Marta es_ES
dc.contributor.author Rodríguez Solovey, Leisa Natacha es_ES
dc.contributor.author Berbel Tornero, Ana es_ES
dc.contributor.author Dietrich, Daniela es_ES
dc.contributor.author FERNÁNDEZ LÓPEZ, MARIA ANGELES es_ES
dc.contributor.author MADUEÑO ALBI, FRANCISCO es_ES
dc.contributor.author Bennett, Malcolm J. es_ES
dc.contributor.author Rodríguez Egea, Pedro Luís es_ES
dc.date.accessioned 2019-07-12T20:00:20Z
dc.date.available 2019-07-12T20:00:20Z
dc.date.issued 2018 es_ES
dc.identifier.uri http://hdl.handle.net/10251/123530
dc.description.abstract [EN] The phytohormone abscisic acid (ABA) plays a key role regulating root growth, root system architecture, and root adaptive responses, such as hydrotropism. The molecular and cellular mechanisms that regulate the action of core ABA signaling components in roots are not fully understood. ABA is perceived through receptors from the PYR/PYL/RCAR family and PP2C coreceptors. PYL8/RCAR3 plays a nonredundant role in regulating primary and lateral root growth. Here we demonstrate that ABA specifically stabilizes PYL8 compared with other ABA receptors and induces accumulation of PYL8 in root nuclei. This requires ABA perception by PYL8 and leads to diminished ubiquitination of PYL8 in roots. The ABA agonist quinabactin, which promotes root ABA signaling through dimeric receptors, fails to stabilize the monomeric receptor PYL8. Moreover, a PYL8 mutant unable to bind ABA and inhibit PP2C is not stabilized by the ligand, whereas a PYL85KR mutant is more stable than PYL8 at endogenous ABA concentrations. The PYL8 transcript was detected in the epidermis and stele of the root meristem; however, the PYL8 protein was also detected in adjacent tissues. Expression of PYL8 driven by tissue-specific promoters revealed movement to adjacent tissues. Hence both inter- and intracellular trafficking of PYL8 appears to occur in the root apical meristem. Our findings reveal a non-cell-autonomous mechanism for hormone receptors and help explain the nonredundant role of PYL8-mediated root ABA signaling. es_ES
dc.description.sponsorship Work in the P.L.R. and F.M. laboratories was supported by the Ministerio de Ciencia e Innovacion, Fondo Europeo de Desarrollo Regional and Consejo Superior de Investigaciones Cientificas Grants BIO2014-52537-R and BIO2017-82503-R (to P.L.R.) and BIO2015-64307-R (to F.M.). J.L.-J. was supported by a Juan de la Cierva contract from Ministerio de Economia y Competitividad (MINECO) and by the Marie Sklodowska-Curie Action H2020-MSCA-IF-2015-707477. B.B.-P. was funded by Programa VALi+d GVA APOSTD/2017/039. J.J. was supported by a FPI contract from MINECO and M.A.F. by a Formacion de Profesorado Universitario contract from MINECO. D.D. and M.J.B. were supported by Biotechnology and Biological Sciences Research Council Grant BB/M002136/1 and Leverhulme Trust Grant RPG-2016-409. es_ES
dc.language Inglés es_ES
dc.publisher Proceedings of the National Academy of Sciences es_ES
dc.relation.ispartof Proceedings of the National Academy of Sciences of the United States of America (Online) es_ES
dc.rights Reconocimiento - No comercial (by-nc) es_ES
dc.subject ABA es_ES
dc.subject ABA biosensor es_ES
dc.subject PYL8 es_ES
dc.subject Non-cell-autonomous es_ES
dc.subject Root es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title PYL8 mediates ABA perception in the root through non-cell-autonomous and ligand-stabilization-based mechanisms es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1073/pnas.1815410115 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/707477/EU/Drought discovery to improve drought tolerance in crops/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BIO2014-52537-R/ES/REGULACION DE LA SEÑALIZACION DEL ABA MEDIANTE MECHANISMOS QUE AFECTAN LOCALIZACION SUBCELULAR, VIDA MEDIA Y ACTIVIDAD DE RECEPTORES PARA REFORZAR TOLERANCIA VEGETAL A SEQUIA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UKRI//BB%2FM002136%2F1/GB/Hydro-patterning: a novel mechanism controlling root branchingHydro-patterning: a novel mechanism controlling root branching/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BIO2015-64307-R/ES/CONTROL GENETICO DE LA ARQUITECTURA DE LA INFLORESCENCIA DE LEGUMINOSAS: NUEVOS GENES PARA LA MEJORA DE SU RENDIMIENTO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//APOSTD%2F2017%2F039/
dc.relation.projectID info:eu-repo/grantAgreement/Leverhulme Trust//RPG-2016-409/
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-82503-R/ES/REGULACION DE LA SEÑALIZACION DEL ABA Y TOLERANCIA A SEQUIA MEDIANTE E3 UBIQUITIN LIGASAS QUE REGULAN EL RECAMBIO DE RECEPTORES Y FOSFATASAS 2C/
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Belda-Palazón, B.; Gonzalez-Garcia, M.; Lozano Juste, J.; Coego Gonzalez, A.; Antoni-Alandes, R.; Julian-Valenzuela, J.; Peirats-Llobet, M.... (2018). PYL8 mediates ABA perception in the root through non-cell-autonomous and ligand-stabilization-based mechanisms. Proceedings of the National Academy of Sciences of the United States of America (Online). 115(50):E11857-E11863. https://doi.org/10.1073/pnas.1815410115 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1073/pnas.1815410115 es_ES
dc.description.upvformatpinicio E11857 es_ES
dc.description.upvformatpfin E11863 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 115 es_ES
dc.description.issue 50 es_ES
dc.identifier.eissn 1091-6490 es_ES
dc.identifier.pmid 30482863 en_EN
dc.identifier.pmcid PMC6294950 en_EN
dc.relation.pasarela S\374265 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder UK Research and Innovation es_ES
dc.contributor.funder Biotechnology and Biological Sciences Research Council, Reino Unido
dc.contributor.funder Leverhulme Trust
dc.contributor.funder Generalitat Valenciana
dc.contributor.funder European Commission
dc.contributor.funder Agencia Estatal de Investigación es_ES
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