dc.contributor.author |
Belda-Palazón, Borja
|
es_ES |
dc.contributor.author |
Gonzalez-Garcia, Mary-Paz
|
es_ES |
dc.contributor.author |
LOZANO JUSTE, JORGE
|
es_ES |
dc.contributor.author |
Coego Gonzalez, Alberto
|
es_ES |
dc.contributor.author |
Antoni-Alandes, Regina
|
es_ES |
dc.contributor.author |
Julian-Valenzuela, Jose
|
es_ES |
dc.contributor.author |
Peirats-Llobet, Marta
|
es_ES |
dc.contributor.author |
Rodríguez Solovey, Leisa Natacha
|
es_ES |
dc.contributor.author |
Berbel Tornero, Ana
|
es_ES |
dc.contributor.author |
Dietrich, Daniela
|
es_ES |
dc.contributor.author |
FERNÁNDEZ LÓPEZ, MARIA ANGELES
|
es_ES |
dc.contributor.author |
MADUEÑO ALBI, FRANCISCO
|
es_ES |
dc.contributor.author |
Bennett, Malcolm J.
|
es_ES |
dc.contributor.author |
Rodríguez Egea, Pedro Luís
|
es_ES |
dc.date.accessioned |
2019-07-12T20:00:20Z |
|
dc.date.available |
2019-07-12T20:00:20Z |
|
dc.date.issued |
2018 |
es_ES |
dc.identifier.uri |
http://hdl.handle.net/10251/123530 |
|
dc.description.abstract |
[EN] The phytohormone abscisic acid (ABA) plays a key role regulating root growth, root system architecture, and root adaptive responses, such as hydrotropism. The molecular and cellular mechanisms that regulate the action of core ABA signaling components in roots are not fully understood. ABA is perceived through receptors from the PYR/PYL/RCAR family and PP2C coreceptors. PYL8/RCAR3 plays a nonredundant role in regulating primary and lateral root growth. Here we demonstrate that ABA specifically stabilizes PYL8 compared with other ABA receptors and induces accumulation of PYL8 in root nuclei. This requires ABA perception by PYL8 and leads to diminished ubiquitination of PYL8 in roots. The ABA agonist quinabactin, which promotes root ABA signaling through dimeric receptors, fails to stabilize the monomeric receptor PYL8. Moreover, a PYL8 mutant unable to bind ABA and inhibit PP2C is not stabilized by the ligand, whereas a PYL85KR mutant is more stable than PYL8 at endogenous ABA concentrations. The PYL8 transcript was detected in the epidermis and stele of the root meristem; however, the PYL8 protein was also detected in adjacent tissues. Expression of PYL8 driven by tissue-specific promoters revealed movement to adjacent tissues. Hence both inter- and intracellular trafficking of PYL8 appears to occur in the root apical meristem. Our findings reveal a non-cell-autonomous mechanism for hormone receptors and help explain the nonredundant role of PYL8-mediated root ABA signaling. |
es_ES |
dc.description.sponsorship |
Work in the P.L.R. and F.M. laboratories was supported by the Ministerio de Ciencia e Innovacion, Fondo Europeo de Desarrollo Regional and Consejo Superior de Investigaciones Cientificas Grants BIO2014-52537-R and BIO2017-82503-R (to P.L.R.) and BIO2015-64307-R (to F.M.). J.L.-J. was supported by a Juan de la Cierva contract from Ministerio de Economia y Competitividad (MINECO) and by the Marie Sklodowska-Curie Action H2020-MSCA-IF-2015-707477. B.B.-P. was funded by Programa VALi+d GVA APOSTD/2017/039. J.J. was supported by a FPI contract from MINECO and M.A.F. by a Formacion de Profesorado Universitario contract from MINECO. D.D. and M.J.B. were supported by Biotechnology and Biological Sciences Research Council Grant BB/M002136/1 and Leverhulme Trust Grant RPG-2016-409. |
es_ES |
dc.language |
Inglés |
es_ES |
dc.publisher |
Proceedings of the National Academy of Sciences |
es_ES |
dc.relation.ispartof |
Proceedings of the National Academy of Sciences of the United States of America (Online) |
es_ES |
dc.rights |
Reconocimiento - No comercial (by-nc) |
es_ES |
dc.subject |
ABA |
es_ES |
dc.subject |
ABA biosensor |
es_ES |
dc.subject |
PYL8 |
es_ES |
dc.subject |
Non-cell-autonomous |
es_ES |
dc.subject |
Root |
es_ES |
dc.subject.classification |
BIOQUIMICA Y BIOLOGIA MOLECULAR |
es_ES |
dc.title |
PYL8 mediates ABA perception in the root through non-cell-autonomous and ligand-stabilization-based mechanisms |
es_ES |
dc.type |
Artículo |
es_ES |
dc.identifier.doi |
10.1073/pnas.1815410115 |
es_ES |
dc.relation.projectID |
info:eu-repo/grantAgreement/EC/H2020/707477/EU/Drought discovery to improve drought tolerance in crops/ |
es_ES |
dc.relation.projectID |
info:eu-repo/grantAgreement/MINECO//BIO2014-52537-R/ES/REGULACION DE LA SEÑALIZACION DEL ABA MEDIANTE MECHANISMOS QUE AFECTAN LOCALIZACION SUBCELULAR, VIDA MEDIA Y ACTIVIDAD DE RECEPTORES PARA REFORZAR TOLERANCIA VEGETAL A SEQUIA/ |
es_ES |
dc.relation.projectID |
info:eu-repo/grantAgreement/UKRI//BB%2FM002136%2F1/GB/Hydro-patterning: a novel mechanism controlling root branchingHydro-patterning: a novel mechanism controlling root branching/ |
es_ES |
dc.relation.projectID |
info:eu-repo/grantAgreement/MINECO//BIO2015-64307-R/ES/CONTROL GENETICO DE LA ARQUITECTURA DE LA INFLORESCENCIA DE LEGUMINOSAS: NUEVOS GENES PARA LA MEJORA DE SU RENDIMIENTO/ |
es_ES |
dc.relation.projectID |
info:eu-repo/grantAgreement/GVA//APOSTD%2F2017%2F039/ |
|
dc.relation.projectID |
info:eu-repo/grantAgreement/Leverhulme Trust//RPG-2016-409/ |
|
dc.relation.projectID |
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-82503-R/ES/REGULACION DE LA SEÑALIZACION DEL ABA Y TOLERANCIA A SEQUIA MEDIANTE E3 UBIQUITIN LIGASAS QUE REGULAN EL RECAMBIO DE RECEPTORES Y FOSFATASAS 2C/ |
|
dc.rights.accessRights |
Abierto |
es_ES |
dc.contributor.affiliation |
Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes |
es_ES |
dc.contributor.affiliation |
Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia |
es_ES |
dc.description.bibliographicCitation |
Belda-Palazón, B.; Gonzalez-Garcia, M.; Lozano Juste, J.; Coego Gonzalez, A.; Antoni-Alandes, R.; Julian-Valenzuela, J.; Peirats-Llobet, M.... (2018). PYL8 mediates ABA perception in the root through non-cell-autonomous and ligand-stabilization-based mechanisms. Proceedings of the National Academy of Sciences of the United States of America (Online). 115(50):E11857-E11863. https://doi.org/10.1073/pnas.1815410115 |
es_ES |
dc.description.accrualMethod |
S |
es_ES |
dc.relation.publisherversion |
https://doi.org/10.1073/pnas.1815410115 |
es_ES |
dc.description.upvformatpinicio |
E11857 |
es_ES |
dc.description.upvformatpfin |
E11863 |
es_ES |
dc.type.version |
info:eu-repo/semantics/publishedVersion |
es_ES |
dc.description.volume |
115 |
es_ES |
dc.description.issue |
50 |
es_ES |
dc.identifier.eissn |
1091-6490 |
es_ES |
dc.identifier.pmid |
30482863 |
en_EN |
dc.identifier.pmcid |
PMC6294950 |
en_EN |
dc.relation.pasarela |
S\374265 |
es_ES |
dc.contributor.funder |
Ministerio de Economía y Competitividad |
es_ES |
dc.contributor.funder |
UK Research and Innovation |
es_ES |
dc.contributor.funder |
Biotechnology and Biological Sciences Research Council, Reino Unido |
|
dc.contributor.funder |
Leverhulme Trust |
|
dc.contributor.funder |
Generalitat Valenciana |
|
dc.contributor.funder |
European Commission |
|
dc.contributor.funder |
Agencia Estatal de Investigación |
es_ES |
dc.description.references |
Ubeda-Tomás, S., Beemster, G. T. S., & Bennett, M. J. (2012). Hormonal regulation of root growth: integrating local activities into global behaviour. Trends in Plant Science, 17(6), 326-331. doi:10.1016/j.tplants.2012.02.002 |
es_ES |
dc.description.references |
Bao, Y., Aggarwal, P., Robbins, N. E., Sturrock, C. J., Thompson, M. C., Tan, H. Q., … Dinneny, J. R. (2014). Plant roots use a patterning mechanism to position lateral root branches toward available water. Proceedings of the National Academy of Sciences, 111(25), 9319-9324. doi:10.1073/pnas.1400966111 |
es_ES |
dc.description.references |
Dietrich, D., Pang, L., Kobayashi, A., Fozard, J. A., Boudolf, V., Bhosale, R., … Bennett, M. J. (2017). Root hydrotropism is controlled via a cortex-specific growth mechanism. Nature Plants, 3(6). doi:10.1038/nplants.2017.57 |
es_ES |
dc.description.references |
Harris, J. (2015). Abscisic Acid: Hidden Architect of Root System Structure. Plants, 4(3), 548-572. doi:10.3390/plants4030548 |
es_ES |
dc.description.references |
Spollen, W. G., LeNoble, M. E., Samuels, T. D., Bernstein, N., & Sharp, R. E. (2000). Abscisic Acid Accumulation Maintains Maize Primary Root Elongation at Low Water Potentials by Restricting Ethylene Production. Plant Physiology, 122(3), 967-976. doi:10.1104/pp.122.3.967 |
es_ES |
dc.description.references |
Sharp, R. E. (2004). Root growth maintenance during water deficits: physiology to functional genomics. Journal of Experimental Botany, 55(407), 2343-2351. doi:10.1093/jxb/erh276 |
es_ES |
dc.description.references |
Deak, K. I., & Malamy, J. (2005). Osmotic regulation of root system architecture. The Plant Journal, 43(1), 17-28. doi:10.1111/j.1365-313x.2005.02425.x |
es_ES |
dc.description.references |
Gonzalez-Guzman, M., Pizzio, G. A., Antoni, R., Vera-Sirera, F., Merilo, E., Bassel, G. W., … Rodriguez, P. L. (2012). Arabidopsis PYR/PYL/RCAR Receptors Play a Major Role in Quantitative Regulation of Stomatal Aperture and Transcriptional Response to Abscisic Acid. The Plant Cell, 24(6), 2483-2496. doi:10.1105/tpc.112.098574 |
es_ES |
dc.description.references |
Duan, L., Dietrich, D., Ng, C. H., Chan, P. M. Y., Bhalerao, R., Bennett, M. J., & Dinneny, J. R. (2013). Endodermal ABA Signaling Promotes Lateral Root Quiescence during Salt Stress in Arabidopsis Seedlings. The Plant Cell, 25(1), 324-341. doi:10.1105/tpc.112.107227 |
es_ES |
dc.description.references |
Feng, W., Lindner, H., Robbins, N. E., & Dinneny, J. R. (2016). Growing Out of Stress: The Role of Cell- and Organ-Scale Growth Control in Plant Water-Stress Responses. The Plant Cell, 28(8), 1769-1782. doi:10.1105/tpc.16.00182 |
es_ES |
dc.description.references |
Geng, Y., Wu, R., Wee, C. W., Xie, F., Wei, X., Chan, P. M. Y., … Dinneny, J. R. (2013). A Spatio-Temporal Understanding of Growth Regulation during the Salt Stress Response in Arabidopsis. The Plant Cell, 25(6), 2132-2154. doi:10.1105/tpc.113.112896 |
es_ES |
dc.description.references |
Takahashi, N., Goto, N., Okada, K., & Takahashi, H. (2002). Hydrotropism in abscisic acid, wavy, and gravitropic mutants of Arabidopsis thaliana. Planta, 216(2), 203-211. doi:10.1007/s00425-002-0840-3 |
es_ES |
dc.description.references |
Antoni, R., Gonzalez-Guzman, M., Rodriguez, L., Peirats-Llobet, M., Pizzio, G. A., Fernandez, M. A., … Rodriguez, P. L. (2012). PYRABACTIN RESISTANCE1-LIKE8 Plays an Important Role for the Regulation of Abscisic Acid Signaling in Root. Plant Physiology, 161(2), 931-941. doi:10.1104/pp.112.208678 |
es_ES |
dc.description.references |
Barberon, M., Vermeer, J. E. M., De Bellis, D., Wang, P., Naseer, S., Andersen, T. G., … Geldner, N. (2016). Adaptation of Root Function by Nutrient-Induced Plasticity of Endodermal Differentiation. Cell, 164(3), 447-459. doi:10.1016/j.cell.2015.12.021 |
es_ES |
dc.description.references |
Ondzighi-Assoume, C. A., Chakraborty, S., & Harris, J. M. (2016). Environmental Nitrate Stimulates Abscisic Acid Accumulation in Arabidopsis Root Tips by Releasing It from Inactive Stores. The Plant Cell, 28(3), 729-745. doi:10.1105/tpc.15.00946 |
es_ES |
dc.description.references |
Irigoyen, M. L., Iniesto, E., Rodriguez, L., Puga, M. I., Yanagawa, Y., Pick, E., … Rubio, V. (2014). Targeted Degradation of Abscisic Acid Receptors Is Mediated by the Ubiquitin Ligase Substrate Adaptor DDA1 in Arabidopsis. The Plant Cell, 26(2), 712-728. doi:10.1105/tpc.113.122234 |
es_ES |
dc.description.references |
Bueso, E., Rodriguez, L., Lorenzo-Orts, L., Gonzalez-Guzman, M., Sayas, E., Muñoz-Bertomeu, J., … Rodriguez, P. L. (2014). The single-subunit RING-type E3 ubiquitin ligase RSL1 targets PYL4 and PYR1 ABA receptors in plasma membrane to modulate abscisic acid signaling. The Plant Journal, 80(6), 1057-1071. doi:10.1111/tpj.12708 |
es_ES |
dc.description.references |
Knoblich, J. A. (2005). Pins for spines. Nature Cell Biology, 7(12), 1057-1058. doi:10.1038/ncb1205-1057 |
es_ES |
dc.description.references |
Zhang, H., Han, W., De Smet, I., Talboys, P., Loya, R., Hassan, A., … Wang, M.-H. (2010). ABA promotes quiescence of the quiescent centre and suppresses stem cell differentiation in the Arabidopsis primary root meristem. The Plant Journal, 64(5), 764-774. doi:10.1111/j.1365-313x.2010.04367.x |
es_ES |
dc.description.references |
Belda-Palazon, B., Rodriguez, L., Fernandez, M. A., Castillo, M.-C., Anderson, E. M., Gao, C., … Rodriguez, P. L. (2016). FYVE1/FREE1 Interacts with the PYL4 ABA Receptor and Mediates Its Delivery to the Vacuolar Degradation Pathway. The Plant Cell, 28(9), 2291-2311. doi:10.1105/tpc.16.00178 |
es_ES |
dc.description.references |
Yu, F., Lou, L., Tian, M., Li, Q., Ding, Y., Cao, X., … Xie, Q. (2016). ESCRT-I Component VPS23A Affects ABA Signaling by Recognizing ABA Receptors for Endosomal Degradation. Molecular Plant, 9(12), 1570-1582. doi:10.1016/j.molp.2016.11.002 |
es_ES |
dc.description.references |
Santiago, J., Rodrigues, A., Saez, A., Rubio, S., Antoni, R., Dupeux, F., … Rodriguez, P. L. (2009). Modulation of drought resistance by the abscisic acid receptor PYL5 through inhibition of clade A PP2Cs. The Plant Journal, 60(4), 575-588. doi:10.1111/j.1365-313x.2009.03981.x |
es_ES |
dc.description.references |
Szostkiewicz, I., Richter, K., Kepka, M., Demmel, S., Ma, Y., Korte, A., … Grill, E. (2010). Closely related receptor complexes differ in their ABA selectivity and sensitivity. The Plant Journal, 61(1), 25-35. doi:10.1111/j.1365-313x.2009.04025.x |
es_ES |
dc.description.references |
Okamoto, M., Peterson, F. C., Defries, A., Park, S.-Y., Endo, A., Nambara, E., … Cutler, S. R. (2013). Activation of dimeric ABA receptors elicits guard cell closure, ABA-regulated gene expression, and drought tolerance. Proceedings of the National Academy of Sciences, 110(29), 12132-12137. doi:10.1073/pnas.1305919110 |
es_ES |
dc.description.references |
Cao, M., Liu, X., Zhang, Y., Xue, X., Zhou, X. E., Melcher, K., … Xu, Y. (2013). An ABA-mimicking ligand that reduces water loss and promotes drought resistance in plants. Cell Research, 23(8), 1043-1054. doi:10.1038/cr.2013.95 |
es_ES |
dc.description.references |
Castillo, M.-C., Lozano-Juste, J., González-Guzmán, M., Rodriguez, L., Rodriguez, P. L., & León, J. (2015). Inactivation of PYR/PYL/RCAR ABA receptors by tyrosine nitration may enable rapid inhibition of ABA signaling by nitric oxide in plants. Science Signaling, 8(392), ra89-ra89. doi:10.1126/scisignal.aaa7981 |
es_ES |
dc.description.references |
Wu, S., & Gallagher, K. L. (2014). The movement of the non-cell-autonomous transcription factor, SHORT-ROOT relies on the endomembrane system. The Plant Journal, 80(3), 396-409. doi:10.1111/tpj.12640 |
es_ES |
dc.description.references |
Nakajima, K., Sena, G., Nawy, T., & Benfey, P. N. (2001). Intercellular movement of the putative transcription factor SHR in root patterning. Nature, 413(6853), 307-311. doi:10.1038/35095061 |
es_ES |
dc.description.references |
Gallagher, K. L., Paquette, A. J., Nakajima, K., & Benfey, P. N. (2004). Mechanisms Regulating SHORT-ROOT Intercellular Movement. Current Biology, 14(20), 1847-1851. doi:10.1016/j.cub.2004.09.081 |
es_ES |
dc.description.references |
Pálfy, M., Reményi, A., & Korcsmáros, T. (2012). Endosomal crosstalk: meeting points for signaling pathways. Trends in Cell Biology, 22(9), 447-456. doi:10.1016/j.tcb.2012.06.004 |
es_ES |
dc.description.references |
Christmann, A., Hoffmann, T., Teplova, I., Grill, E., & Müller, A. (2004). Generation of Active Pools of Abscisic Acid Revealed by In Vivo Imaging of Water-Stressed Arabidopsis. Plant Physiology, 137(1), 209-219. doi:10.1104/pp.104.053082 |
es_ES |
dc.description.references |
Kim, T.-H., Hauser, F., Ha, T., Xue, S., Böhmer, M., Nishimura, N., … Schroeder, J. I. (2011). Chemical Genetics Reveals Negative Regulation of Abscisic Acid Signaling by a Plant Immune Response Pathway. Current Biology, 21(11), 990-997. doi:10.1016/j.cub.2011.04.045 |
es_ES |
dc.description.references |
Waadt, R., Hitomi, K., Nishimura, N., Hitomi, C., Adams, S. R., Getzoff, E. D., & Schroeder, J. I. (2014). FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis. eLife, 3. doi:10.7554/elife.01739 |
es_ES |
dc.description.references |
Jones, A. M., Danielson, J. Å., ManojKumar, S. N., Lanquar, V., Grossmann, G., & Frommer, W. B. (2014). Abscisic acid dynamics in roots detected with genetically encoded FRET sensors. eLife, 3. doi:10.7554/elife.01741 |
es_ES |
dc.description.references |
Zhao, Y., Xing, L., Wang, X., Hou, Y.-J., Gao, J., Wang, P., … Zhu, J.-K. (2014). The ABA Receptor PYL8 Promotes Lateral Root Growth by Enhancing MYB77-Dependent Transcription of Auxin-Responsive Genes. Science Signaling, 7(328), ra53-ra53. doi:10.1126/scisignal.2005051 |
es_ES |
dc.description.references |
Peirats-Llobet, M., Han, S.-K., Gonzalez-Guzman, M., Jeong, C. W., Rodriguez, L., Belda-Palazon, B., … Rodriguez, P. L. (2016). A Direct Link between Abscisic Acid Sensing and the Chromatin-Remodeling ATPase BRAHMA via Core ABA Signaling Pathway Components. Molecular Plant, 9(1), 136-147. doi:10.1016/j.molp.2015.10.003 |
es_ES |
dc.description.references |
Moes, D., Himmelbach, A., Korte, A., Haberer, G., & Grill, E. (2008). Nuclear localization of the mutant protein phosphatase abi1 is required for insensitivity towards ABA responses in Arabidopsis. The Plant Journal, 54(5), 806-819. doi:10.1111/j.1365-313x.2008.03454.x |
es_ES |
dc.description.references |
Lynch, T., Erickson, B. J., & Finkelstein, R. R. (2012). Direct interactions of ABA-insensitive(ABI)-clade protein phosphatase(PP)2Cs with calcium-dependent protein kinases and ABA response element-binding bZIPs may contribute to turning off ABA response. Plant Molecular Biology, 80(6), 647-658. doi:10.1007/s11103-012-9973-3 |
es_ES |