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dc.contributor.author | Eichmeier, A. | es_ES |
dc.contributor.author | Pecenka, J. | es_ES |
dc.contributor.author | Penazova, E. | es_ES |
dc.contributor.author | Baranek, M. | es_ES |
dc.contributor.author | Català, Santiago | es_ES |
dc.contributor.author | León Santana, Maela | es_ES |
dc.contributor.author | Armengol Fortí, Josep | es_ES |
dc.contributor.author | Gramaje Pérez, David | es_ES |
dc.date.accessioned | 2019-09-13T20:01:40Z | |
dc.date.available | 2019-09-13T20:01:40Z | |
dc.date.issued | 2018 | es_ES |
dc.identifier.issn | 1754-5048 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/125662 | |
dc.description.abstract | [EN] The ecology of total fungal communities in grapevine is so far largely derived from studies on culture-dependent methods or cultivation-independent rDNA approaches. Sequencing the ribosomal RNA transcripts (rRNA) would rather reveal the functionally and metabolically active important taxa of the fungal community and provide insights into its activity in the wood. The present study investigated changes in the potentially active fungal communities of internal grapevine wood after Hot-Water Treatment (HWT) in planting material from Czech Republic and Spain at two different times during the propagation process and from two plant zones. We examined fungal communities using both traditional isolation into culture and high-throughput amplicon sequencing (HTAS) of the internal transcribed spacer 2 (ITS2) region in extracted total RNA. HTAS from metatranscriptomic RNA increased the resolution of the fungal community analysis and revealed a highly diverse mycobiota of grapevine wood compared to the traditional method. Fungal diversity differed between grapevine genotypes and showed a temporal variation over the vegetative period. Grapevine planting materials exhibited high fungal diversity after HWT, which demonstrates that the HWT process does not sterilize the internal wood of grapevine. HWT reduced the infection caused by fungal trunk disease pathogens but was not completely effective in eliminating their growth. This study provides important and practically useful insights into the dynamics of active fungal communities in hot-water treated plants, and represents the first study of active fungal communities on grapevine grafted plants by comparing traditional and next-generation sequencing methods. (C) 2018 Elsevier Ltd and British Mycological Society. All rights reserved. | es_ES |
dc.description.sponsorship | This work was supported by Projects No. LD14051 from the Ministry of Education, Youth and Sports, Czech Republic covered by COST Action FA1303, and No. CZ.02.1.01/0.0/0.0/16_025/0007314 "Multidisciplinary research to increase application potential of nanomaterials in agricultural practice" from EFRR (Grant provider: Ministerstvo Skolstvi, mladeze a telovychovy Ceske republiky (MSMT). Access to computing and storage facilities owned by parties and projects contributing to the National Grid Infrastructure MetaCentrum provided under the programme "Projects of Large Research, Development, and Innovations Infrastructures" (CESNET LM2015042), is greatly appreciated. David Gramaje was supported by the DOC-INIA program from the National Institute for Agronomic Research (INIA), co-funded by the European Social Fund. The authors are grateful for the technical assistance of Jesus Alfonso Crespo and Aitor Gavara Vidal. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.ispartof | Fungal Ecology | es_ES |
dc.rights | Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) | es_ES |
dc.subject | Endophytic fungi | es_ES |
dc.subject | Grapevine trunk diseases | es_ES |
dc.subject | ITS2 | es_ES |
dc.subject | Metatranscriptomics | es_ES |
dc.subject | Mycobiota | es_ES |
dc.subject | NGS | es_ES |
dc.subject | Plant microbiome | es_ES |
dc.subject.classification | PRODUCCION VEGETAL | es_ES |
dc.title | High-throughput amplicon sequencing-based analysis of active fungal communities inhabiting grapevine after hot-water treatments reveals unexpectedly high fungal diversity | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.funeco.2018.07.011 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MSMT//CZ.02.1.01%2F0.0%2F0.0%2F16_025%2F0007314/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MSMT//LM2015042/CZ/E-infrastruktura CESNET/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Ecosistemas Agroforestales - Departament d'Ecosistemes Agroforestals | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani | es_ES |
dc.description.bibliographicCitation | Eichmeier, A.; Pecenka, J.; Penazova, E.; Baranek, M.; Català, S.; León Santana, M.; Armengol Fortí, J.... (2018). High-throughput amplicon sequencing-based analysis of active fungal communities inhabiting grapevine after hot-water treatments reveals unexpectedly high fungal diversity. Fungal Ecology. 36:26-38. https://doi.org/10.1016/j.funeco.2018.07.011 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://doi.org/10.1016/j.funeco.2018.07.011 | es_ES |
dc.description.upvformatpinicio | 26 | es_ES |
dc.description.upvformatpfin | 38 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 36 | es_ES |
dc.relation.pasarela | S\368869 | es_ES |
dc.contributor.funder | Ministry of Education, Youth and Sports, República Checa | |
dc.contributor.funder | Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria |