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Isolation, molecular identification and quinolone-susceptibility testing of Arcobacter spp. isolated from fresh vegetables in Spain

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Isolation, molecular identification and quinolone-susceptibility testing of Arcobacter spp. isolated from fresh vegetables in Spain

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dc.contributor.author González Pellicer, Ana es_ES
dc.contributor.author Bayas-Morejón, Isidro Favian es_ES
dc.contributor.author Ferrús Pérez, Mª Antonia es_ES
dc.date.accessioned 2019-12-25T21:02:07Z
dc.date.available 2019-12-25T21:02:07Z
dc.date.issued 2017 es_ES
dc.identifier.issn 0740-0020 es_ES
dc.identifier.uri http://hdl.handle.net/10251/133655
dc.description.abstract [EN] Some species of the Arcobacter genus are considered emerging foodborne and waterborne enteropathogens. However, the presence of Arcobacter spp. in vegetables very little is known, because most studies have focused on foods of animal origin. On the other hand, quinolones are considered as first-line drugs for the treatment of infection by campylobacteria in human patients, but few data are currently available about the resistance levels to these antibiotics among Arcobacter species. Therefore, the aim of this study was to investigate the presence and diversity of arcobacters isolated from fresh vegetables such as lettuces, spinaches, chards and cabbages. Resistance to quinolones of the isolates was also investigated. One hundred fresh vegetables samples purchased from seven local retail markets in Valencia (Spain) during eight months were analysed. The study included 41 lettuces, 21 spinaches, 34 chards and 4 cabbages. Samples were analysed by culture and by molecular methods before and after enrichment. By culture, 17 out of 100 analysed samples were Arcobacter positive and twenty-five isolates were obtained from them. Direct detection by PCR was low, with only 4 % Arcobacter spp. positive samples. This percentage increased considerably, up 20 %, after 48 h enrichment. By polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), 17 out of the 25 isolates were identified as A. butzleri and 8 as A. cryaerophilus. Only two A. butzleri isolates showed resistance to levofloxacin and ciprofloxacin. The sequencing of a fragment of the QRDR region of the gyrA gene from the quinolones-resistant isolates revealed the presence of a mutation in position 254 of this gene (C-T transition). This study is the first report about the presence of pathogenic species of Arcobacter spp. in chards and cabbages and confirms that fresh vegetables can act as transmission vehicle to humans. Moreover, the presence of A. butzleri quinolone resistant in vegetables could pose a potential public health risk. es_ES
dc.description.sponsorship This work was supported by an AGL2014-53875-R-AR Research Project from Ministerio de Ciencia y Tecnologia, Spain (National and FEDER funding). es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation MINECO/AGL2014-53875-R-AR es_ES
dc.relation.ispartof Food Microbiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Arcobacter spp. detection es_ES
dc.subject Vegetables es_ES
dc.subject Quinolone resistance es_ES
dc.subject GyrA es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.title Isolation, molecular identification and quinolone-susceptibility testing of Arcobacter spp. isolated from fresh vegetables in Spain es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.fm.2017.02.011 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation González Pellicer, A.; Bayas-Morejón,  F.; Ferrús Pérez, MA. (2017). Isolation, molecular identification and quinolone-susceptibility testing of Arcobacter spp. isolated from fresh vegetables in Spain. Food Microbiology. 65:279-283. https://doi.org/10.1016/j.fm.2017.02.011 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.fm.2017.02.011 es_ES
dc.description.upvformatpinicio 279 es_ES
dc.description.upvformatpfin 283 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 65 es_ES
dc.relation.pasarela S\315937 es_ES
dc.contributor.funder Ministerio de Economía y Empresa es_ES


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