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Photo-click chemistry to create nucleic acids dextran-based microarrays

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Photo-click chemistry to create nucleic acids dextran-based microarrays

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dc.contributor.author Díaz-Betancor, Zeneida es_ES
dc.contributor.author Bañuls Polo, María-José es_ES
dc.contributor.author Maquieira Catala, Angel es_ES
dc.date.accessioned 2020-03-23T08:46:09Z
dc.date.available 2020-03-23T08:46:09Z
dc.date.issued 2019-10 es_ES
dc.identifier.issn 1618-2642 es_ES
dc.identifier.uri http://hdl.handle.net/10251/139153
dc.description The final publication is available at link.springer.com es_ES
dc.description.abstract [EN] In the literature, there are reports of the utilization of various hydrogels to create generic platforms for protein microarray applications. Here, a novel strategy was developed to obtain high-performance microarrays. In it, a dextran hydrogel is used to covalently immobilize oligonucleotides and proteins. This method employs aqueous solutions of dextran methacrylate (Dx-MA), which is a biocompatible photopolymerizable monomer. Capture probes are immobilized inside the hydrogel via a light-induced thiol-acrylate coupling reaction at the same time as the dextran polymer is formed. Hydrogel microarrays based on this technique were prepared on different surfaces, such as a Blu-ray Disk and polycarbonate or alkene-functionalized glass slides, and these systems showed high probe-loading capabilities and good biorecognition yields. This methodology presents advantages such as a low cost, a short analysis time, a low limit of detection, and multiplexing capabilities, among others. Confocal fluorescence microscopy analysis demonstrated that in these hydrogel-based microarrays, receptor immobilization and the biorecognition event occurred within the hydrogel and not merely on the surface. es_ES
dc.description.sponsorship Funding from MINECO through the project BIHOLOG CTQ/2016/75749-R is acknowledged. es_ES
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation.ispartof Analytical and Bioanalytical Chemistry es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Fluorescence microarray es_ES
dc.subject Hydrogel es_ES
dc.subject Dextran es_ES
dc.subject Thiol-acrylate click chemistry es_ES
dc.subject Nucleic acids es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Photo-click chemistry to create nucleic acids dextran-based microarrays es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s00216-019-02050-3 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//CTQ2016-75749-R/ES/BIOSENSORES HOLOGRAFICOS. PRUEBA DE CONCEPTO Y DEMOSTRACION EN APLICACIONES CLINICAS/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Tecnología Nanofotónica - Institut Universitari de Tecnologia Nanofotònica es_ES
dc.description.bibliographicCitation Díaz-Betancor, Z.; Bañuls Polo, M.; Maquieira Catala, A. (2019). Photo-click chemistry to create nucleic acids dextran-based microarrays. Analytical and Bioanalytical Chemistry. 411(25):6745-6754. https://doi.org/10.1007/s00216-019-02050-3 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1007/s00216-019-02050-3 es_ES
dc.description.upvformatpinicio 6745 es_ES
dc.description.upvformatpfin 6754 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 411 es_ES
dc.description.issue 25 es_ES
dc.relation.pasarela S\392676 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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