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Development and evaluation of a real-time PCR assay for quantification of Giardia and Cryptosporidium in sewage samples

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Development and evaluation of a real-time PCR assay for quantification of Giardia and Cryptosporidium in sewage samples

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Alonso Molina, JL.; Amoros, I.; Cañigral Cárcel, I. (2011). Development and evaluation of a real-time PCR assay for quantification of Giardia and Cryptosporidium in sewage samples. Applied Microbiology and Biotechnology. 89(4):1203-1211. https://doi.org/10.1007/s00253-010-2984-6

Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/139922

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Title: Development and evaluation of a real-time PCR assay for quantification of Giardia and Cryptosporidium in sewage samples
Author:
UPV Unit: Universitat Politècnica de València. Instituto Universitario de Ingeniería del Agua y del Medio Ambiente - Institut Universitari d'Enginyeria de l'Aigua i Medi Ambient
Issued date:
Abstract:
[EN] Cryptosporidium and Giardia are major causes of diarrheal disease in humans worldwide and are major causes of protozoan waterborne diseases. Two DNA TaqMan PCR-based Giardia and Cryptosporidium methods targeting a ...[+]
Subjects: Cryptosporidium , Giardia , LightCycler , LNA probe , QPCR , Sewage , TaqMan , DNA , Genes , Nucleic acids , Probes , Polymerase chain reaction , Assay , Diarrheal disease , Gene expression , Genetic analysis , Protozoan , Sensitivity analysis , Species diversity , Waterborne disease , Article , DNA extraction , Gene sequence , Nonhuman , Nucleic acid probe , Quantitative analysis , Real time polymerase chain reaction , Sampling , Cytoskeletal Proteins , Humans , Oligonucleotide Probes , Parasitology , Protozoan Proteins , Sensitivity and Specificity , Protozoa
Copyrigths: Reserva de todos los derechos
Source:
Applied Microbiology and Biotechnology. (issn: 0175-7598 )
DOI: 10.1007/s00253-010-2984-6
Publisher:
SPRINGER
Publisher version: https://doi.org/10.1007/s00253-010-2984-6
Thanks:
This work was supported by the Spanish Ministerio de Ciencia e Innovacion grants AGL2005-07776-C03-03 and AGL2008-05275-C03-03/ALI). Part of this work was also funded by the Fondo Europeo de Desarrollo Regional (Feder) ...[+]
Type: Artículo

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