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Development and evaluation of a real-time PCR assay for quantification of Giardia and Cryptosporidium in sewage samples

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Development and evaluation of a real-time PCR assay for quantification of Giardia and Cryptosporidium in sewage samples

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dc.contributor.author Alonso Molina, José Luís es_ES
dc.contributor.author Amoros, Inmaculada es_ES
dc.contributor.author Cañigral Cárcel, Irene es_ES
dc.date.accessioned 2020-04-01T07:15:20Z
dc.date.available 2020-04-01T07:15:20Z
dc.date.issued 2011 es_ES
dc.identifier.issn 0175-7598 es_ES
dc.identifier.uri http://hdl.handle.net/10251/139922
dc.description.abstract [EN] Cryptosporidium and Giardia are major causes of diarrheal disease in humans worldwide and are major causes of protozoan waterborne diseases. Two DNA TaqMan PCR-based Giardia and Cryptosporidium methods targeting a 74-bp sequence of the ß-giardin Giardia gene and a 151-bp sequence of the COWP Cryptosporidium gene, respectively, were used as models to compare two different LNA/DNA TaqMan probes to improve the detection limit in a real-time PCR assay. The LNA probes were the most sensitive resulting in 0.96 to 1.57 lower C t values than a DNA Giardia TaqMan probe and 0.56 to 2.21 lower than a DNA Cryptosporidium TaqMan probe. Evaluation of TaqMan Giardia and Cryptosporidium probes with LNA substitutions resulted in real-time PCR curves with an earlier C t values than conventional DNA TaqMan probes. In conclusion, the LNA probes could be useful for more sensitive detection limits. © 2011 Springer-Verlag. es_ES
dc.description.sponsorship This work was supported by the Spanish Ministerio de Ciencia e Innovacion grants AGL2005-07776-C03-03 and AGL2008-05275-C03-03/ALI). Part of this work was also funded by the Fondo Europeo de Desarrollo Regional (Feder) grant POICV 2000-2006. We thank staff at the wastewater treatment plants and the Entidad de Saneamiento de Aguas for assistance in sample collection. es_ES
dc.language Inglés es_ES
dc.publisher SPRINGER es_ES
dc.relation FEDER/POICV 2000-2006 es_ES
dc.relation MINISTERIO DE EDUCACION /AGL2005-07776-C03-03 es_ES
dc.relation MINISTERIO DE EDUCACION /AGL2008-05275-C03-03 es_ES
dc.relation.ispartof Applied Microbiology and Biotechnology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Cryptosporidium es_ES
dc.subject Giardia es_ES
dc.subject LightCycler es_ES
dc.subject LNA probe es_ES
dc.subject QPCR es_ES
dc.subject Sewage es_ES
dc.subject TaqMan es_ES
dc.subject DNA es_ES
dc.subject Genes es_ES
dc.subject Nucleic acids es_ES
dc.subject Probes es_ES
dc.subject Polymerase chain reaction es_ES
dc.subject Assay es_ES
dc.subject Diarrheal disease es_ES
dc.subject Gene expression es_ES
dc.subject Genetic analysis es_ES
dc.subject Protozoan es_ES
dc.subject Sensitivity analysis es_ES
dc.subject Species diversity es_ES
dc.subject Waterborne disease es_ES
dc.subject Article es_ES
dc.subject DNA extraction es_ES
dc.subject Gene sequence es_ES
dc.subject Nonhuman es_ES
dc.subject Nucleic acid probe es_ES
dc.subject Quantitative analysis es_ES
dc.subject Real time polymerase chain reaction es_ES
dc.subject Sampling es_ES
dc.subject Cytoskeletal Proteins es_ES
dc.subject Humans es_ES
dc.subject Oligonucleotide Probes es_ES
dc.subject Parasitology es_ES
dc.subject Protozoan Proteins es_ES
dc.subject Sensitivity and Specificity es_ES
dc.subject Protozoa es_ES
dc.title Development and evaluation of a real-time PCR assay for quantification of Giardia and Cryptosporidium in sewage samples es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s00253-010-2984-6 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Ingeniería del Agua y del Medio Ambiente - Institut Universitari d'Enginyeria de l'Aigua i Medi Ambient es_ES
dc.description.bibliographicCitation Alonso Molina, JL.; Amoros, I.; Cañigral Cárcel, I. (2011). Development and evaluation of a real-time PCR assay for quantification of Giardia and Cryptosporidium in sewage samples. Applied Microbiology and Biotechnology. 89(4):1203-1211. https://doi.org/10.1007/s00253-010-2984-6 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1007/s00253-010-2984-6 es_ES
dc.description.upvformatpinicio 1203 es_ES
dc.description.upvformatpfin 1211 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 89 es_ES
dc.description.issue 4 es_ES
dc.relation.pasarela S\213497 es_ES
dc.contributor.funder Ministerio de Educación es_ES
dc.contributor.funder European Regional Development Fund es_ES


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