Ródenas Rochina, J.; Kelly, DJ.; Gómez Ribelles, JL.; Lebourg, MM. (2016). Compositional changes to synthetic biodegradable scaffolds modulate the influence of hydrostatic pressure on chondrogenesis of mesenchymal stem cells. Biomedical Physics & Engineering Express. 2(3). https://doi.org/10.1088/2057-1976/2/3/035005
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/140056
Title:
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Compositional changes to synthetic biodegradable scaffolds modulate the influence of hydrostatic pressure on chondrogenesis of mesenchymal stem cells
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Author:
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Ródenas Rochina, Joaquín
Kelly, Daniel John
Gómez Ribelles, José Luís
Lebourg, Myriam Madeleine
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UPV Unit:
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Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada
Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular
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Issued date:
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Abstract:
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[EN] Mechanical cues such as hydrostatic pressure (HP) are known to regulate mesenchymal stem cell (MSC) differentiation. The fate of such cells is also strongly influenced by their substrate. The objective of this study ...[+]
[EN] Mechanical cues such as hydrostatic pressure (HP) are known to regulate mesenchymal stem cell (MSC) differentiation. The fate of such cells is also strongly influenced by their substrate. The objective of this study was to test how different modifications of polycaprolactone (PCL) scaffolds would influence the response of MSCs to HP. Porcine bone marrow derived MSCs were cultured on PCL, PCL-hyaluronic acid (HA) and PCL-Bioglass (R) (BG) scaffolds for 35 d and stimulated with aHP bioreactor (10 MPa; 1 Hz; 2 h d(-1)). Scaffold composition was found to modulate the response to HP. MSCs seeded onto both PCL and BGscaffolds responded positively to the application of HP, with increases in cartilage extracellular matrix synthesis and a reduction in type I collagen accumulation. This positive effect was not observed onHAscaffolds. The results of this study demonstrate that changes to scaffold composition can have a notable effect on the response of MSCs to bioreactor culture conditions.
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Subjects:
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Mesenchymal stem cells
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Scaffold
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Tissue engineering
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Copyrigths:
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Reserva de todos los derechos
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Source:
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Biomedical Physics & Engineering Express. (eissn:
2057-1976
)
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DOI:
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10.1088/2057-1976/2/3/035005
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Publisher:
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IOP Publishing
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Publisher version:
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https://doi.org/10.1088/2057-1976/2/3/035005
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Project ID:
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info:eu-repo/grantAgreement/EC/FP7/258463/EU
ISCIII/CIBER-BBN
...[+]
info:eu-repo/grantAgreement/EC/FP7/258463/EU
ISCIII/CIBER-BBN
GV/BEFPI/2012/084
SFI/08/YI5/B1336
ERC/258463
GENERALITAT VALENCIANA/ACIF/2010/238
MINECO/MAT2013-46467-C4-1-R
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Thanks:
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Joaquin Rodenas-Rochina acknowledges funding of his PhD and his stay at the Trinity Centre for Bioengineering by the Generalitat Valenciana through ACIF grant (ACIF/2010/238) and BEFPI grant (BEFPI/2012/084) respectively. ...[+]
Joaquin Rodenas-Rochina acknowledges funding of his PhD and his stay at the Trinity Centre for Bioengineering by the Generalitat Valenciana through ACIF grant (ACIF/2010/238) and BEFPI grant (BEFPI/2012/084) respectively. Funding to Daniel Kelly was provided by Science Foundation Ireland (President of Ireland Young Researcher Award: 08/YI5/B1336) and the European Research Council (StemRepair-Project number 258463) Jose L Gomez Ribelles acknowledges the support of the Ministerio de Economia y Competitividad, MINECO, through the MAT2013-46467-C4-1-R project. CIBER-BBN is an initiative funded by the VI National R&D&i Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund.
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Type:
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Artículo
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